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Mouse Anti-FXYD5 Recombinant Antibody (E-12) (CBMAB-D2076-YC)

Provided herein is a Mouse monoclonal antibody, which binds to FXYD Domain Containing Ion Transport Regulator 5 (FXYD5). The antibody can be used for immunoassay techniques, such as WB, IP, IF, ELISA.
See all FXYD5 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
E-12
Antibody Isotype
IgG
Application
WB, IP, IF, ELISA

Basic Information

Specificity
Human
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
FXYD Domain Containing Ion Transport Regulator 5
Introduction
FXYD5 belongs to a family of small membrane proteins that share a 35-amino acid signature sequence domain, beginning with the sequence PFXYD and containing 7 invariant and 6 highly conserved amino acids. The approved human gene nomenclature for the family is FXYD-domain containing ion transport regulator. Mouse FXYD5 has been termed RIC (Related to Ion Channel).
Entrez Gene ID
UniProt ID
Alternative Names
FXYD Domain Containing Ion Transport Regulator 5; Dysadherin; FXYD Domain-Containing Ion Transport Regulator 5; DYSAD; IWU1; Keratinocytes Associated Transmembrane Protein 1;
Function
Involved in down-regulation of E-cadherin which results in reduced cell adhesion. Promotes metastasis.
Biological Process
Ion transport Source: UniProtKB-KW
Microvillus assembly Source: UniProtKB
Negative regulation of calcium-dependent cell-cell adhesion Source: UniProtKB
Regulation of sodium ion transmembrane transporter activity Source: GO_Central
Cellular Location
Membrane
Topology
Extracellular: 22-145
Helical: 146-164
Cytoplasmic: 165-178
PTM
Glycosylated.

Hotta, T., Nariai, Y., Kajitani, N., Kadota, K., Maruyama, R., Tajima, Y., ... & Urano, T. (2023). Generation of the novel anti-FXYD5 monoclonal antibody and its application to the diagnosis of pancreatic and lung cancer. Biochimie.

Tan, X. P., Xiong, B. H., Zhang, Y. X., Wang, S. L., Zuo, Q., & Li, J. (2022). FXYD5 promotes sorafenib resistance through the Akt/mTOR signaling pathway in hepatocellular carcinoma. European Journal of Pharmacology, 931, 175186.

Song, L., Li, X., Sun, Q., & Zhao, Y. (2022). Fxyd5 activates the NF‑κB pathway and is involved in chondrocytes inflammation and extracellular matrix degradation. Molecular Medicine Reports, 25(4), 1-8.

Ding, L., & Li, J. (2022). FXYD domain containing ion transport regulator 5 (FXYD5) silencing promotes cell viability and alleviates inflammatory response in cerulein-induced AR42J cells by blocking JAK2/STAT3 signaling pathway. Bioengineered, 13(2), 2639-2647.

Juurikka, K., Dufour, A., Pehkonen, K., Mainoli, B., Campioni Rodrigues, P., Solis, N., ... & Åström, P. (2021). MMP8 increases tongue carcinoma cell–cell adhesion and diminishes migration via cleavage of anti-adhesive FXYD5. Oncogenesis, 10(5), 44.

Jan, V., Miš, K., Nikolic, N., Dolinar, K., Petrič, M., Bone, A., ... & Pirkmajer, S. (2021). Effect of differentiation, de novo innervation, and electrical pulse stimulation on mRNA and protein expression of Na+, K+-ATPase, FXYD1, and FXYD5 in cultured human skeletal muscle cells. Plos one, 16(2), e0247377.

Liu, Y. K., Jia, Y. J., Liu, S. H., Shi, H. J., & Ma, J. (2021). Low expression of FXYD5 reverses the cisplatin resistance of epithelial ovarian cancer cells. Histology and Histopathology, 36(5), 535-545.

Bai, Y., Li, L. D., Li, J., Chen, R. F., Yu, H. L., Sun, H. F., ... & Lu, X. (2020). A FXYD5/TGF‑β/SMAD positive feedback loop drives epithelial‑to‑mesenchymal transition and promotes tumor growth and metastasis in ovarian cancer. International Journal of Oncology, 56(1), 301-314.

Tassi, R. A., Gambino, A., Ardighieri, L., Bignotti, E., Todeschini, P., Romani, C., ... & Ravaggi, A. (2019). FXYD5 (Dysadherin) upregulation predicts shorter survival and reveals platinum resistance in high-grade serous ovarian cancer patients. British Journal of Cancer, 121(7), 584-592.

Besso, M. J., Rosso, M., Lapyckyj, L., Moiola, C. P., Matos, M. L., Mercogliano, M. F., ... & Vazquez-Levin, M. H. (2019). FXYD5/Dysadherin, a biomarker of endometrial cancer myometrial invasion and aggressiveness: its relationship with TGF-β1 and NF-κB pathways. Frontiers in Oncology, 9, 1306.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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