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Rat Anti-FZD6 Recombinant Antibody (CBXF-2693) (CBMAB-F0129-CQ)

This product is a rat antibody that recognizes FZD6. The antibody CBXF-2693 can be used for immunoassay techniques such as: WB.
See all FZD6 antibodies

Summary

Host Animal
Rat
Specificity
Mouse
Clone
CBXF-2693
Antibody Isotype
IgG2a
Application
WB

Basic Information

Immunogen
NS0-derived recombinant mouse Frizzled-6, His19-Val153 (predicted), Accession # Q61089
Specificity
Mouse
Antibody Isotype
IgG2a
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Lyophilized
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with trehalose
Concentration
LYOPH
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Frizzled Class Receptor 6
Introduction
This gene represents a member of the 'frizzled' gene family, which encode 7-transmembrane domain proteins that are receptors for Wnt signaling proteins. The protein encoded by this family member contains a signal peptide, a cysteine-rich domain in the N-terminal extracellular region, and seven transmembrane domains, but unlike other family members, this protein does not contain a C-terminal PDZ domain-binding motif. This protein functions as a negative regulator of the canonical Wnt/beta-catenin signaling cascade, thereby inhibiting the processes that trigger oncogenic transformation, cell proliferation, and inhibition of apoptosis. Alternative splicing results in multiple transcript variants, some of which do not encode a protein with a predicted signal peptide.
Entrez Gene ID
14368
UniProt ID
Q61089
Alternative Names
Frizzled Class Receptor 6; Frizzled 6, Seven Transmembrane Spanning Receptor; Frizzled Family Receptor 6; FZ-6; HFZ6; Seven Transmembrane Helix Receptor; Frizzled (Drosophila) Homolog 6;
Function
Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Together with FZD3, is involved in the neural tube closure and plays a role in the regulation of the establishment of planar cell polarity (PCP), particularly in the orientation of asymmetric bundles of stereocilia on the apical faces of a subset of auditory and vestibular sensory cells located in the inner ear (By similarity).
Biological Process
Canonical Wnt signaling pathway Source: GO_Central
Cell proliferation in midbrain Source: Ensembl
Embryonic nail plate morphogenesis Source: Ensembl
Establishment of body hair planar orientation Source: Ensembl
Hair follicle development Source: Ensembl
Inner ear morphogenesis Source: Ensembl
Midbrain morphogenesis Source: ParkinsonsUK-UCL
Negative regulation of canonical Wnt signaling pathway Source: BHF-UCL
Negative regulation of DNA-binding transcription factor activity Source: BHF-UCL
Neural tube closure Source: Ensembl
Non-canonical Wnt signaling pathway Source: BHF-UCL
Platelet activation Source: Ensembl
Wnt signaling pathway, planar cell polarity pathway Source: ParkinsonsUK-UCL
Cellular Location
Endoplasmic reticulum membrane; Cell membrane; Apical cell membrane; Membrane; Cell surface; Cytoplasmic vesicle membrane. Colocalizes with FZD3 at the apical face of cells (By similarity). Localizes to the endoplasmic reticulum membrane in the presence of LMBR1L (By similarity).
Involvement in disease
Nail disorder, non-syndromic congenital, 1 (NDNC1):
An autosomal recessive nail disorder characterized by a variable degree of onychauxis (thick nails), hyponychia, and onycholysis of all nails, with claw-shaped fingernails in some individuals. No other anomalies of ectodermal tissues, including hair, teeth, sweat glands, or skin, are noted, and individuals with dysplastic nails have normal hearing and normal psychomotor development. Rare non-synonymous variants in FZD6 may contribute to neural tube defects, congenital malformations of the central nervous system and adjacent structures related to defective neural tube closure during the first trimester of pregnancy.
Topology
Extracellular: 19-201
Helical: 202-222
Cytoplasmic: 223-233
Helical: 234-254
Extracellular: 255-284
Helical: 285-305
Cytoplasmic: 306-324
Helical: 325-345
Extracellular: 346-370
Helical: 371-391
Cytoplasmic: 392-416
Helical: 417-437
Extracellular: 438-473
Helical: 474-494
Cytoplasmic: 495-706
PTM
Ubiquitinated by ZNRF3, leading to its degradation by the proteasome.

Lu, Y., Liu, Y. K., Wan, F. Y., Shi, S., & Tao, R. (2022). CircSmg5 stimulates the osteogenic differentiation of bone marrow mesenchymal stem cells by targeting the miR‐194‐5p/Fzd6 axis and beta‐catenin signaling. Environmental Toxicology, 37(3), 593-602.

Wang, T., Li, W., Ye, B., Zhang, S., Lei, X., & Zhang, D. (2021). FTO-stabilized lncRNA HOXC13-AS epigenetically upregulated FZD6 and activated Wnt/β-catenin signaling to drive cervical cancer proliferation, invasion, and EMT. J buon, 26, 1279-1291.

Zhang, J., Wang, J. L., Zhang, C. Y., Ma, Y. F., Zhao, R., & Wang, Y. Y. (2020). The prognostic role of FZD6 in esophageal squamous cell carcinoma patients. Clinical and Translational Oncology, 22, 1172-1179.

Yuan, Y., Wang, Q., Ma, S. L., Xu, L. Q., Liu, M. Y., Han, B., ... & Cao, F. F. (2019). lncRNA PCAT-1 interacting with FZD6 contributes to the malignancy of acute myeloid leukemia cells through activating Wnt/β-catenin signaling pathway. American journal of translational research, 11(11), 7104.

Beghini, A., & Postorino, G. (2019). FZD6 (Frizzled class receptor 6). ATLAS OF GENETICS AND CYTOGENETICS IN ONCOLOGY AND HAEMATOLOGY, 23(5), 112-119.

Xu, C., Tian, G., Jiang, C., Xue, H., Kuerbanjiang, M., Sun, L., ... & Xu, Q. (2019). NPTX2 promotes colorectal cancer growth and liver metastasis by the activation of the canonical Wnt/β-catenin pathway via FZD6. Cell death & disease, 10(3), 217.

Han, K., Lang, T., Zhang, Z., Zhang, Y., Sun, Y., Shen, Z., ... & Min, D. (2018). Luteolin attenuates Wnt signaling via upregulation of FZD6 to suppress prostate cancer stemness revealed by comparative proteomics. Scientific Reports, 8(1), 8537.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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