Human Recombinant FZD6 protein, His Tag (V2LY-0526-LY4201)

Name: Human Recombinant FZD6 protein, His Tag
SKU: V2LY-0526-LY4201
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant FZD6 protein, His Tag consist of Amino Acid: 1-153 and predicts a molecular mass of 17.1 kDa.

Molecule Mass

17.1 kDa

Sequence

Amino Acid: 1-153

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>95% as determined by SDS-PAGE.

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Frizzled Class Receptor 6

Function

Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Together with FZD3, is involved in the neural tube closure and plays a role in the regulation of the establishment of planar cell polarity (PCP), particularly in the orientation of asymmetric bundles of stereocilia on the apical faces of a subset of auditory and vestibular sensory cells located in the inner ear (By similarity).

Biological Process

Canonical Wnt signaling pathway Source: GO_Central
Cell proliferation in midbrain Source: Ensembl
Embryonic nail plate morphogenesis Source: Ensembl
Establishment of body hair planar orientation Source: Ensembl
Hair follicle development Source: Ensembl
Inner ear morphogenesis Source: Ensembl
Midbrain morphogenesis Source: ParkinsonsUK-UCL
Negative regulation of canonical Wnt signaling pathway Source: BHF-UCL
Negative regulation of DNA-binding transcription factor activity Source: BHF-UCL
Neural tube closure Source: Ensembl
Non-canonical Wnt signaling pathway Source: BHF-UCL
Platelet activation Source: Ensembl
Wnt signaling pathway, planar cell polarity pathway Source: ParkinsonsUK-UCL

Cellular Location

Endoplasmic reticulum membrane; Cell membrane; Apical cell membrane; Membrane; Cell surface; Cytoplasmic vesicle membrane. Colocalizes with FZD3 at the apical face of cells (By similarity). Localizes to the endoplasmic reticulum membrane in the presence of LMBR1L (By similarity).

Involvement in disease

Nail disorder, non-syndromic congenital, 1 (NDNC1):
An autosomal recessive nail disorder characterized by a variable degree of onychauxis (thick nails), hyponychia, and onycholysis of all nails, with claw-shaped fingernails in some individuals. No other anomalies of ectodermal tissues, including hair, teeth, sweat glands, or skin, are noted, and individuals with dysplastic nails have normal hearing and normal psychomotor development. Rare non-synonymous variants in FZD6 may contribute to neural tube defects, congenital malformations of the central nervous system and adjacent structures related to defective neural tube closure during the first trimester of pregnancy.

Topology

Extracellular: 19-201
Helical: 202-222
Cytoplasmic: 223-233
Helical: 234-254
Extracellular: 255-284
Helical: 285-305
Cytoplasmic: 306-324
Helical: 325-345
Extracellular: 346-370
Helical: 371-391
Cytoplasmic: 392-416
Helical: 417-437
Extracellular: 438-473
Helical: 474-494
Cytoplasmic: 495-706

PTM

Ubiquitinated by ZNRF3, leading to its degradation by the proteasome.

Lu, Y., Liu, Y. K., Wan, F. Y., Shi, S., & Tao, R. (2022). CircSmg5 stimulates the osteogenic differentiation of bone marrow mesenchymal stem cells by targeting the miR‐194‐5p/Fzd6 axis and beta‐catenin signaling. Environmental Toxicology, 37(3), 593-602.

Wang, T., Li, W., Ye, B., Zhang, S., Lei, X., & Zhang, D. (2021). FTO-stabilized lncRNA HOXC13-AS epigenetically upregulated FZD6 and activated Wnt/β-catenin signaling to drive cervical cancer proliferation, invasion, and EMT. J buon, 26, 1279-1291.

Zhang, J., Wang, J. L., Zhang, C. Y., Ma, Y. F., Zhao, R., & Wang, Y. Y. (2020). The prognostic role of FZD6 in esophageal squamous cell carcinoma patients. Clinical and Translational Oncology, 22, 1172-1179.

Yuan, Y., Wang, Q., Ma, S. L., Xu, L. Q., Liu, M. Y., Han, B., ... & Cao, F. F. (2019). lncRNA PCAT-1 interacting with FZD6 contributes to the malignancy of acute myeloid leukemia cells through activating Wnt/β-catenin signaling pathway. American journal of translational research, 11(11), 7104.

Beghini, A., & Postorino, G. (2019). FZD6 (Frizzled class receptor 6). ATLAS OF GENETICS AND CYTOGENETICS IN ONCOLOGY AND HAEMATOLOGY, 23(5), 112-119.

Xu, C., Tian, G., Jiang, C., Xue, H., Kuerbanjiang, M., Sun, L., ... & Xu, Q. (2019). NPTX2 promotes colorectal cancer growth and liver metastasis by the activation of the canonical Wnt/β-catenin pathway via FZD6. Cell death & disease, 10(3), 217.

Han, K., Lang, T., Zhang, Z., Zhang, Y., Sun, Y., Shen, Z., ... & Min, D. (2018). Luteolin attenuates Wnt signaling via upregulation of FZD6 to suppress prostate cancer stemness revealed by comparative proteomics. Scientific Reports, 8(1), 8537.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant FZD6 protein, Fc Tag (CAT#: V2LY-0526-LY4202)

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Isotype control

For research use only. Not intended for any clinical use.

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