Rat Anti-FZD7 (AA 25-173) Recombinant Antibody (CBXF-2268) (CBMAB-F0719-CQ)

Name: Rat Anti-FZD7 (AA 25-173) Recombinant Antibody (CBXF-2268)
SKU: CBMAB-F0719-CQ
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rat

Clone

CBXF-2268

Application

FC, IF

Immunogen

NS0-derived recombinant mouse Frizzled-7 Gln33-Leu185

Specificity

Human, Mouse

Antibody Isotype

IgG2a

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 5% trehalose

Concentration

0.5 mg/mL

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

AA 25-173

More Infomation

Target

Full Name

Frizzled Class Receptor 7

Introduction

Members of the 'frizzled' gene family encode 7-transmembrane domain proteins that are receptors for Wnt signaling proteins. The FZD7 protein contains an N-terminal signal sequence, 10 cysteine residues typical of the cysteine-rich extracellular domain of Fz family members, 7 putative transmembrane domains, and an intracellular C-terminal tail with a PDZ domain-binding motif. FZD7 gene expression may downregulate APC function and enhance beta-catenin-mediated signals in poorly differentiated human esophageal carcinomas.

Entrez Gene ID

Human	8324
Mouse	14369

UniProt ID

Human	O75084
Mouse	Q61090

Alternative Names

Frizzled Class Receptor 7; Frizzled 7, Seven Transmembrane Spanning Receptor; Frizzled Family Receptor 7; Fz-7; FzE3; HFz7;

Function

Receptor for Wnt proteins. Most frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. Activation by WNT8 induces expression of beta-catenin target genes (By similarity).

Following ligand activation, binds to CCDC88C/DAPLE which displaces DVL1 from FZD7 and leads to inhibition of canonical Wnt signaling, activation of G-proteins by CCDC88C and triggering of non-canonical Wnt responses (PubMed:26126266).

May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues.

(Microbial infection) Acts as a receptor for C.difficile toxin TcdB in the colonic epithelium.

Biological Process

Canonical Wnt signaling pathway Source: UniProtKB
Cellular response to retinoic acid Source: UniProtKB
Mesenchymal to epithelial transition Source: BHF-UCL
Negative regulation of cardiac muscle cell differentiation Source: BHF-UCL
Negative regulation of cell-substrate adhesion Source: BHF-UCL
Negative regulation of ectodermal cell fate specification Source: BHF-UCL
Neuron differentiation Source: UniProtKB
Non-canonical Wnt signaling pathway via JNK cascade Source: BHF-UCL
Positive regulation of epithelial cell proliferation involved in wound healing Source: BHF-UCL
Positive regulation of JNK cascade Source: BHF-UCL
Positive regulation of phosphorylation Source: BHF-UCL
Positive regulation of transcription, DNA-templated Source: BHF-UCL
Regulation of canonical Wnt signaling pathway Source: BHF-UCL
Regulation of transcription, DNA-templated Source: BHF-UCL
Skeletal muscle satellite cell maintenance involved in skeletal muscle regeneration Source: Ensembl
Somatic stem cell division Source: Ensembl
Stem cell population maintenance Source: BHF-UCL
Substrate adhesion-dependent cell spreading Source: Ensembl
T cell differentiation in thymus Source: Ensembl
Wnt signaling pathway, planar cell polarity pathway Source: ParkinsonsUK-UCL

Cellular Location

Cell membrane; Endosome membrane. Associated to the plasma membrane in the presence of FZD7 and phosphatidylinositol 4,5-bisphosphate (PIP2). Localized in recycling endosomes in other conditions.

Topology

Extracellular: 33-256
Helical: 257-277
Cytoplasmic: 278-288
Helical: 289-309
Extracellular: 310-336
Helical: 337-357
Cytoplasmic: 358-379
Helical: 380-400
Extracellular: 401-423
Helical: 424-444
Cytoplasmic: 445-470
Helical: 471-491
Extracellular: 492-528
Helical: 529-549
Cytoplasmic: 550-574

PTM

Ubiquitinated by ZNRF3, leading to its degradation by the proteasome.

Zhang, W., Liao, Y., Lou, J., Zhuang, M., Yan, H., Li, Q., ... & Sun, Y. (2022). CircRNA_Maml2 promotes the proliferation and migration of intestinal epithelial cells after severe burns by regulating the miR-93-3p/FZD7/Wnt/β-catenin pathway. Burns & Trauma, 10.

Do, M., Wu, C. C., Sonavane, P. R., Juarez, E. F., Adams, S. R., Ross, J., ... & Willert, K. (2022). A FZD7-specific Antibody-Drug Conjugate Induces Ovarian Tumor Regression in Preclinical Models. Molecular cancer therapeutics, 21(1), 113-124.

Zhang, Z., Gao, S., Xu, Y., & Zhao, C. (2021). Regulation of ABCG2 expression by Wnt5a through FZD7 in human pancreatic cancer cells. Molecular Medicine Reports, 23(1), 1-1.

Liu, Q., Guan, Y., Li, Z., Wang, Y., Liu, Y., Cui, R., & Wang, Y. (2019). miR-504 suppresses mesenchymal phenotype of glioblastoma by directly targeting the FZD7-mediated Wnt–β-catenin pathway. Journal of Experimental & Clinical Cancer Research, 38(1), 1-18.

Chen, Z., & Duan, X. (2018). hsa_circ_0000177-miR-638-FZD7-Wnt signaling cascade contributes to the malignant behaviors in glioma. DNA and cell biology, 37(9), 791-797.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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For research use only. Not intended for any clinical use.

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