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Rat Anti-FZD7 Recombinant Antibody (CBXF-3270) (CBMAB-F3964-CQ)

This product is a rat antibody that recognizes FZD7. The antibody CBXF-3270 can be used for immunoassay techniques such as: ELISA, FC, ICC.
See all FZD7 antibodies

Summary

Host Animal
Rat
Specificity
Human, Mouse
Clone
CBXF-3270
Antibody Isotype
IgG2a
Application
ELISA, FC, ICC

Basic Information

Immunogen
Recombinant protein corresponding to aa33-185 from mouse Frizzled-7 expressed in NS0 cells (Q61090)
Specificity
Human, Mouse
Antibody Isotype
IgG2a
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Lyophilized
Concentration
LYOPH
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Frizzled Class Receptor 7
Introduction
Members of the 'frizzled' gene family encode 7-transmembrane domain proteins that are receptors for Wnt signaling proteins. The FZD7 protein contains an N-terminal signal sequence, 10 cysteine residues typical of the cysteine-rich extracellular domain of Fz family members, 7 putative transmembrane domains, and an intracellular C-terminal tail with a PDZ domain-binding motif. FZD7 gene expression may downregulate APC function and enhance beta-catenin-mediated signals in poorly differentiated human esophageal carcinomas.
Entrez Gene ID
Human8324
Mouse14369
UniProt ID
HumanO75084
MouseQ61090
Alternative Names
Frizzled Class Receptor 7; Frizzled 7, Seven Transmembrane Spanning Receptor; Frizzled Family Receptor 7; Fz-7; FzE3; HFz7;
Function
Receptor for Wnt proteins. Most frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. Activation by WNT8 induces expression of beta-catenin target genes (By similarity).

Following ligand activation, binds to CCDC88C/DAPLE which displaces DVL1 from FZD7 and leads to inhibition of canonical Wnt signaling, activation of G-proteins by CCDC88C and triggering of non-canonical Wnt responses (PubMed:26126266).

May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues.

(Microbial infection) Acts as a receptor for C.difficile toxin TcdB in the colonic epithelium.
Biological Process
Canonical Wnt signaling pathway Source: UniProtKB
Cellular response to retinoic acid Source: UniProtKB
Mesenchymal to epithelial transition Source: BHF-UCL
Negative regulation of cardiac muscle cell differentiation Source: BHF-UCL
Negative regulation of cell-substrate adhesion Source: BHF-UCL
Negative regulation of ectodermal cell fate specification Source: BHF-UCL
Neuron differentiation Source: UniProtKB
Non-canonical Wnt signaling pathway via JNK cascade Source: BHF-UCL
Positive regulation of epithelial cell proliferation involved in wound healing Source: BHF-UCL
Positive regulation of JNK cascade Source: BHF-UCL
Positive regulation of phosphorylation Source: BHF-UCL
Positive regulation of transcription, DNA-templated Source: BHF-UCL
Regulation of canonical Wnt signaling pathway Source: BHF-UCL
Regulation of transcription, DNA-templated Source: BHF-UCL
Skeletal muscle satellite cell maintenance involved in skeletal muscle regeneration Source: Ensembl
Somatic stem cell division Source: Ensembl
Stem cell population maintenance Source: BHF-UCL
Substrate adhesion-dependent cell spreading Source: Ensembl
T cell differentiation in thymus Source: Ensembl
Wnt signaling pathway, planar cell polarity pathway Source: ParkinsonsUK-UCL
Cellular Location
Cell membrane; Endosome membrane. Associated to the plasma membrane in the presence of FZD7 and phosphatidylinositol 4,5-bisphosphate (PIP2). Localized in recycling endosomes in other conditions.
Topology
Extracellular: 33-256
Helical: 257-277
Cytoplasmic: 278-288
Helical: 289-309
Extracellular: 310-336
Helical: 337-357
Cytoplasmic: 358-379
Helical: 380-400
Extracellular: 401-423
Helical: 424-444
Cytoplasmic: 445-470
Helical: 471-491
Extracellular: 492-528
Helical: 529-549
Cytoplasmic: 550-574
PTM
Ubiquitinated by ZNRF3, leading to its degradation by the proteasome.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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