Sign in or Register   Sign in or Register
  |  

Mouse Anti-G3BP1 Recombinant Antibody (2F3) (CBMAB-G1547-LY)

This product is antibody recognizes G3BP1. The antibody 2F3 immunoassay techniques such as: IF, WB.
See all G3BP1 antibodies
Published Data
Fig.1 Ras-GTPase-activating protein SH3 domain-binding protein 1 (G3BP1) is up-regulated in gastric cancer at the post-transcriptional level.
...View More
Fig.2 Immunohistochemical staining of RasGTPase-activating protein SH3 domain-binding protein 1(G3BP1) in sections of gastric cancer.
...View More
Fig.3 Ras-GTPaseactivating protein SH3 domain-binding protein 1(G3BP1) expression correlates with extracellular signalrelated kinase (ERK) and AKT activation in gastric cancer
...View More
Fig.4 SG proteins and polyadenylated mRNA are well recruited to SGs despite impaired SG aggregation.
...View More

Summary

Host Animal
Mouse
Specificity
Human
Clone
2F3
Antibody Isotype
IgG1
Application
IF, WB

Basic Information

Immunogen
Recombinant protein corresponding to aa 214 - 303 of human G3BP
Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Concentration
0.5 mg/mL
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
G3BP1
Introduction
This gene encodes one of the DNA-unwinding enzymes which prefers partially unwound 3'-tailed substrates and can also unwind partial RNA/DNA and RNA/RNA duplexes in an ATP-dependent fashion. This enzyme is a member of the heterogeneous nuclear RNA-binding proteins and is also an element of the Ras signal transduction pathway. It binds specifically to the Ras-GTPase-activating protein by associating with its SH3 domain. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. [provided by RefSeq, Jul 2008]
G3BP1 (G3BP Stress Granule Assembly Factor 1) is a Protein Coding gene.


Gene Ontology (GO) annotations related to this gene include nucleic acid binding and mRNA binding.
An important paralog of this gene is G3BP2.
May be a regulated effector of stress granule assembly. Phosphorylation-dependent sequence-specific endoribonuclease in vitro. Cleaves exclusively between cytosine and adenine and cleaves MYC mRNA preferentially at the 3-UTR. ATP- and magnesium-dependent helicase. Unwinds preferentially partial DNA and RNA duplexes having a 17 bp annealed portion and either a hanging 3 tail or hanging tails at both 5- and 3-ends. Unwinds DNA/DNA, RNA/DNA, and RNA/RNA substrates with comparable efficiency. Acts unidirectionally by moving in the 5 to 3 direction along the bound single-stranded DNA.
Entrez Gene ID
10146
UniProt ID
Q13283
Alternative Names
G3BP Stress Granule Assembly Factor 1; Ras-GTPase-Activating Protein SH3-Domain-Binding Protein; GTPase Activating Protein (SH3 Domain) Binding Protein 1; GAP SH3 Domain-Binding Protein 1; ATP-Dependent DNA Helicase VIII; DNA Helicase VIII; G3BP-1; G3BP; Ras GTPase-Activating Protein-Binding Protein 1;
Function
ATP- and magnesium-dependent helicase that plays an essential role in innate immunity (PubMed:30510222).

Participates in the DNA-triggered cGAS/STING pathway by promoting the DNA binding and activation of CGAS. Enhances also DDX58-induced type I interferon production probably by helping DDX58 at sensing pathogenic RNA (PubMed:30804210).

In addition, plays an essential role in stress granule formation (PubMed:12642610, PubMed:20180778, PubMed:23279204).

Unwinds preferentially partial DNA and RNA duplexes having a 17 bp annealed portion and either a hanging 3' tail or hanging tails at both 5'- and 3'-ends (PubMed:9889278).

Unwinds DNA/DNA, RNA/DNA, and RNA/RNA substrates with comparable efficiency (PubMed:9889278).

Acts unidirectionally by moving in the 5' to 3' direction along the bound single-stranded DNA (PubMed:9889278).

Phosphorylation-dependent sequence-specific endoribonuclease in vitro (PubMed:11604510).

Cleaves exclusively between cytosine and adenine and cleaves MYC mRNA preferentially at the 3'-UTR (PubMed:11604510).
Biological Process
Defense response to virus Source: UniProtKB
Innate immune response Source: UniProtKB-KW
Negative regulation of canonical Wnt signaling pathway Source: Ensembl
Positive regulation of stress granule assembly Source: UniProtKB
Positive regulation of type I interferon production Source: UniProtKB
Ras protein signal transduction Source: ProtInc
Stress granule assembly Source: UniProtKB
Cellular Location
Cytosol; Nucleus; Perikaryon; Stress granule. Cytoplasmic in proliferating cells (PubMed:11604510). Cytosolic and partially nuclear in resting cells (PubMed:11604510). Recruited to stress granules in response to arsenite treatment (PubMed:12642610, PubMed:20180778). The unphosphorylated form is recruited to stress granules (PubMed:12642610). HRAS signaling contributes to this process by regulating G3BP dephosphorylation (PubMed:12642610).
PTM
(Microbial infection) Cleaved by human enterovirus 71; this cleavage induces the disassembly of cytoplasmic stress granules (PubMed:30006004). Cleaved by Foot-and-mouth disease virus; this cleavage suppresses the formation of cytoplasmic stress granules (PubMed:30404792).
Phosphorylated exclusively on serine residues. Hyperphosphorylated in quiescent fibroblasts. Hypophosphorylation leads to a decrease in endoribonuclease activity (By similarity). RASA1-dependent phosphorylation of Ser-149 induces a conformational change that prevents self-association. Dephosphorylation after HRAS activation is required for stress granule assembly. Ser-149 phosphorylation induces partial nuclear localization.
Arg-435 is dimethylated, probably to asymmetric dimethylarginine.
Ask a question We look forward to hearing from you.
0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Loading...
Have you used Mouse Anti-G3BP1 Recombinant Antibody (2F3)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon $30 eGift Card
Submit a review
Loading...
For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Documents

Contact us

  • Tel: (USA)
  • (UK)
  • Fax:
  • Email:

Submit A Review

Go to
Compare