Mouse Anti-GABPA Recombinant Antibody (CBFYH-0213) (V2LY-1225-LY518)

Name: Mouse Anti-GABPA Recombinant Antibody (CBFYH-0213)
SKU: V2LY-1225-LY518
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-GABPA Recombinant Antibody (CBFYH-0213) (V2LY-1225-LY518) in IP

Semi-quantitative PCR products from DNA prepared by ChIP assays. Lanes 1 and 5: No DNA control. Lanes 2 and 6: The Input lanes correspond to PCR products derived from a 1/10 dilution of chromatin prior to immunoprecipitation. Lanes 3 and 7: The IgG lanes correspond to PCRs containing chromatin immunoprecipitated with antibodies against control IgG. Lanes 4 and 8: The NRF-2α and YY-1 lanes correspond to PCRs containing chromatin immunoprecipitated with antibodies against NRF-2α and YY-1, respectively. All the amplified products represent a 254-bp region (from position -66 to -319) containing both the NRF-2 and YY-1 binding sites of the PREPL-C2ORF34 bidirectional gene pair. ...View More

Huang, C. C., & Chang, W. S. (2009). Cooperation between NRF-2 and YY-1 transcription factors is essential for triggering the expression of the PREPL-C2ORF34 bidirectional gene pair. BMC molecular biology, 10, 67.

Datasheet

SDS

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Basic Information

Host Animal

Mouse

Clone

CBFYH-0213

Application

WB, IHC, IF, ELISA, IHC-P

Immunogen

Amino acids 1-180 of human GABP-α.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

Application	Note
ELISA	1:100-1:1,000
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
IHC-P	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, gelatin

Preservative

Sodium azide

Concentration

0.2 mg/ml

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

GA binding protein transcription factor subunit alpha

Entrez Gene ID

Human	2551
Mouse	14390
Rat	363735

UniProt ID

Human	Q06546
Mouse	Q00422
Rat	D4ACQ9

Function

Transcription factor capable of interacting with purine rich repeats (GA repeats). Necessary for the expression of the Adenovirus E4 gene.

Biological Process

Blastocyst formation Source: Ensembl
Cell differentiation Source: GO_Central
Cellular response to dopamine Source: CAFA
Negative regulation of megakaryocyte differentiation Source: Ensembl
Negative regulation of transcription by RNA polymerase II Source: Ensembl
Positive regulation of gene expression Source: CAFA
Positive regulation of transcription by RNA polymerase II Source: UniProtKB
Regulation of transcription by RNA polymerase II Source: GO_Central

Cellular Location

Nucleus

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Mouse Anti-GABPA Recombinant Antibody (CBFYH-0213) (CAT#: CBMAB-H1086-FY)

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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