Mouse Anti-GADD45GIP1 Recombinant Antibody (EG1278) (V2LY-1225-LY599)

Name: Mouse Anti-GADD45GIP1 Recombinant Antibody (EG1278)
SKU: V2LY-1225-LY599
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-GADD45GIP1 Recombinant Antibody (EG1278) (V2LY-1225-LY599) in WB

Western blot analysis of DHFR expression of WT and KO mouse endothelial cells. DHFR level was quantified densitometrically. Data are presented with three independent experiments as the mean ± SEM. * p < 0.05 compared with WT group. ...View More

Lee, I., Piao, S., Kim, S., Nagar, H., Choi, S. J., Jeon, B. H., Oh, S. H., Irani, K., & Kim, C. S. (2021). CRIF1 Deficiency Increased Homocysteine Production by Disrupting Dihydrofolate Reductase Expression in Vascular Endothelial Cells. Antioxidants (Basel, Switzerland), 10(11), 1645.

Mouse Anti-GADD45GIP1 Recombinant Antibody (EG1278) (V2LY-1225-LY599) in WB

A,B) Proteins required for mitochondrial oxidative phosphorylation (Cytochrome c oxidase subunit 4, COX4) and the intramitochondrial production of mtDNA-encoded OXPHOS subunits (CRIF1) were measured using Western blot. ...View More

Lyu, A. R., Kim, T. H., Shin, S. A., Kim, E. H., Yu, Y., Gajbhiye, A., Kwon, H. C., Je, A. R., Huh, Y. H., Park, M. J., & Park, Y. H. (2021). Hearing Impairment in a Mouse Model of Diabetes Is Associated with Mitochondrial Dysfunction, Synaptopathy, and Activation of the Intrinsic Apoptosis Pathway. International journal of molecular sciences, 22(16), 8807.

Datasheet

SDS

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Basic Information

Host Animal

Mouse

Clone

EG1278

Application

WB, IP, IF, ELISA

Immunogen

Full length of mouse CRIF1.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

Application	Note
ELISA	1:100-1:1,000
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, gelatin

Preservative

Sodium azide

Concentration

0.2 mg/ml

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

GADD45G Interacting Protein 1

Entrez Gene ID

90480

UniProt ID

Q8TAE8

Function

Acts as a negative regulator of G1 to S cell cycle phase progression by inhibiting cyclin-dependent kinases. Inhibitory effects are additive with GADD45 proteins but occurs also in the absence of GADD45 proteins. Acts as a repressor of the orphan nuclear receptor NR4A1 by inhibiting AB domain-mediated transcriptional activity. May be involved in the hormone-mediated regulation of NR4A1 transcriptional activity. May play a role in mitochondrial protein synthesis.

Biological Process

Cell cycle Source: UniProtKB-KW
Regulation of cell cycle Source: GO_Central

Cellular Location

Mitochondrion; Nucleus. Using N-terminally tagged constructs, has been found in the nucleus (PubMed:12482659). C-terminally tagged constructs are targeted exclusively to mitochondria (PubMed:22453275). This discrepancy may be explained by masking of a potential N-terminal mitochondrial targeting signal by the tag (PubMed:22453275).

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Rabbit Anti-GADD45GIP1 Recombinant Antibody (EG1278) (CAT#: CBMAB-EN1542-LY)

Related Products

Rabbit Anti-GADD45GIP1 Recombinant Antibody (EG1278) (CAT#: CBMAB-EN1542-LY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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