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Mouse Anti-GBP1-5 Recombinant Antibody (EG1296) (V2LY-1225-LY836)

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Published Data
V2LY-1225-LY836-1.png
Mouse Anti-GBP1-5 Recombinant Antibody (EG1296) (V2LY-1225-LY836) in IF
Representative images of hGBPs foci formation in hGBP1 KO cells upon lysosomal damage. IFN-γ-primed A549 NT and hGBP1 KO cells were treated with PBS or LLOMe for 1 h and cells were then stained with antibody against hGBP1-5. ...View More
Buijze, H., Brinkmann, V., Hurwitz, R., Dorhoi, A., Kaufmann, S. H. E., & Pei, G. (2023). Human GBP1 Is Involved in the Repair of Damaged Phagosomes/Endolysosomes. International journal of molecular sciences, 24(11), 9701.
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Mouse Anti-GBP1-5 Recombinant Antibody (EG1296) (V2LY-1225-LY836) in IHC
Representative images of GBP1-5, CD8, and programmed cell death ligand 1 (PD-L1) immunohistochemistry (IHC) staining in hepatocellular carcinoma (HCC) samples were shown (scale bars: 100 μm). ...View More
Ning, Y., Fang, S., Fang, J., Lin, K., Nie, H., Xiong, P., Qiu, P., Zhao, Q., Wang, H., & Wang, F. (2023). Guanylate-binding proteins signature predicts favorable prognosis, immune-hot microenvironment, and immunotherapy response in hepatocellular carcinoma. Cancer medicine, 12(16), 17504-17521.
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Mouse Anti-GBP1-5 Recombinant Antibody (EG1296) (V2LY-1225-LY836) in WB
Macrophages (pre-treated with 100 U/ml IFN-γ for 24 h) infected ME49wt or ME49Δcdpk3 parasites were lysed. The lysates were detected by western blot with the indicated antibody. GAPDH was used as a loading control. ...View More
Wu, M., An, R., Zhou, N., Chen, Y., Cai, H., Yan, Q., Wang, R., Luo, Q., Yu, L., Chen, L., & Du, J. (2022). Toxoplasma gondii CDPK3 Controls the Intracellular Proliferation of Parasites in Macrophages. Frontiers in immunology, 13, 905142.
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Mouse Anti-GBP1-5 Recombinant Antibody (EG1296) (V2LY-1225-LY836) in IF
Cells (pre -treated with 100U/ml IFN-γ for 24 h) were infected with ME49wt or ME49Δcdpk3 parasites (moi =3), fixed at 4-6 h post-infection, and incubated with rabbit anti-CDPK3 antibody (red), mouse anti-IRG-47 antibody (green) or mouse anti-GBP1-5 antibody (green), and DAPI (blue). (Scale bar:5μm) ...View More
Wu, M., An, R., Zhou, N., Chen, Y., Cai, H., Yan, Q., Wang, R., Luo, Q., Yu, L., Chen, L., & Du, J. (2022). Toxoplasma gondii CDPK3 Controls the Intracellular Proliferation of Parasites in Macrophages. Frontiers in immunology, 13, 905142.
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Mouse Anti-GBP1-5 Recombinant Antibody (EG1296) (V2LY-1225-LY836) in IF
Immunofluorescent images of GFAP+ (green) astrocyte and pro-inflammatory reactive markers (C3d+ or Gbp2+, red). Scale bar, 20 μm. ...View More
Wan, T., Zhu, W., Zhao, Y., Zhang, X., Ye, R., Zuo, M., Xu, P., Huang, Z., Zhang, C., Xie, Y., & Liu, X. (2022). Astrocytic phagocytosis contributes to demyelination after focal cortical ischemia in mice. Nature communications, 13(1), 1134.
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Mouse Anti-GBP1-5 Recombinant Antibody (EG1296) (V2LY-1225-LY836) in Immunoblot
Immunoblotting analyses of GFAP, LCN2, C3d and Gbp2 expressions in WT and Lcn2-/- mice (n = 5 mice; mean ± S.D.; two-way ANOVA, Tukey post hoc test or repeated-measures t-test; ***P < 0.001 vs. ...View More
Wan, T., Zhu, W., Zhao, Y., Zhang, X., Ye, R., Zuo, M., Xu, P., Huang, Z., Zhang, C., Xie, Y., & Liu, X. (2022). Astrocytic phagocytosis contributes to demyelination after focal cortical ischemia in mice. Nature communications, 13(1), 1134.
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Mouse Anti-GBP1-5 Recombinant Antibody (EG1296) (V2LY-1225-LY836) in IF
(A, B, C, D, E, F, G) Confocal microscope images (left) and the graphs (right) represent the localization of Irga6 (A), Gbp1 (B), Gbp2 (C), Gbp1-5 (D), Irgb10 (E), p62 (F), and ubiquitin (G) (red) to T. gondii vacuoles (green), and DAPI (blue) at 4 h postinfection in IFN-γ-treated WT and Irgb6 KO MEFs. (H) Confocal microscope images represent the colocalization of Irgb6 (red) and ubiquitin (cyan blue) to T. gondii vacuoles (green), and DAPI (blue) at 4 h postinfection in IFN-γ-treated WT MEFs. ...View More
Lee, Y., Yamada, H., Pradipta, A., Ma, J. S., Okamoto, M., Nagaoka, H., Takashima, E., Standley, D. M., Sasai, M., Takei, K., & Yamamoto, M. (2019). Initial phospholipid-dependent Irgb6 targeting to Toxoplasma gondii vacuoles mediates host defense. Life science alliance, 3(1), e201900549.
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Mouse Anti-GBP1-5 Recombinant Antibody (EG1296) (V2LY-1225-LY836) in IF
Structured illumination micrographs of HeLa cells expressing GFP-tagged GBP1 treated with IFNg and infected with mCherry-expressing S. flexneri at 2 hr p.i. and stained for K48-linked ubiquitin. Scale bar, 1 mm. ...View More
Wandel, M. P., Pathe, C., Werner, E. I., Ellison, C. J., Boyle, K. B., von der Malsburg, A., Rohde, J., & Randow, F. (2017). GBPs Inhibit Motility of Shigella flexneri but Are Targeted for Degradation by the Bacterial Ubiquitin Ligase IpaH9.8. Cell host & microbe, 22(4), 507-518.e5.
Datasheet
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Basic Information

Host Animal
Mouse
Clone
EG1296
Application
WB, IP, IF, ELISA, IHC-P
Immunogen
Amino acids 1-300 mapping at the N-terminus of human GBP1.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
ApplicationNote
ELISA1:100-1:1,000
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
IHC-P1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, gelatin
Preservative
Sodium azide
Concentration
0.2 mg/ml
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
More Infomation

Target

Full Name
Guanylate Binding Protein 3
Entrez Gene ID
2635
UniProt ID
Q9H0R5
Function
Exhibits antiviral activity against influenza virus.
Biological Process
Cellular response to interferon-gamma Source: GO_Central
Cellular response to interleukin-1 Source: UniProtKB
Cellular response to tumor necrosis factor Source: UniProtKB
Defense response to virus Source: UniProtKB-KW
Cellular Location
Cytoplasm; Golgi apparatus membrane; Perinuclear region. Heterodimers with GBP1, GBP2 and GBP5 localize in the compartment of the prenylated GBPs: with GBP1 in a vesicle-like compartment, with GBP2, around the nucleus and with GBP-5, at the Golgi apparatus.
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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Rabbit Anti-GBP3 Recombinant Antibody (EG1296) (CAT#: CBMAB-EN1567-LY)

Related Products

Rabbit Anti-GBP3 Recombinant Antibody (EG1296) (CAT#: CBMAB-EN1567-LY)

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For research use only. Not intended for any clinical use.

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