Mouse Anti-GEM Recombinant Antibody (4B12) (V2LY-1225-LY1044)

Name: Mouse Anti-GEM Recombinant Antibody (4B12)
SKU: V2LY-1225-LY1044
Rating: 5 (1 reviews)

See all GEM antibodies

Compare Online Inquiry

Published Data

Mouse Anti-GEM Recombinant Antibody (4B12) (V2LY-1225-LY1044) in IF

Immunofluorescence staining of Gem (red signals) and β-catenin (green signals) expression in AGS cells induced (even numbers) or not induced (odd numbers) with 1 μg/mL doxycycline and grown under (A) Col1 conditions (B) eCol1 conditions and (C) eMedia conditions. Nuclei were stained with DAPI (blue signal). ...View More

Albano, F., Russi, S., Laurino, S., Mazzone, P., Di Paola, G., Zoppoli, P., Amendola, E., Balzamo, C., Bartolo, O., Ciuffi, M., Ignomirelli, O., Sgambato, A., Galasso, R., De Felice, M., Falco, G., & Calice, G. (2024). Representing ECM composition and EMT pathways in gastric cancer using a new metastatic gene signature. Frontiers in cell and developmental biology, 12, 1481818.

Mouse Anti-GEM Recombinant Antibody (4B12) (V2LY-1225-LY1044) in WB

Protein expressions of Tax, Fos, Rora, Rrad, Gem, and Tnfaip6 were validated by western blotting. Actin was used as a control. ...View More

Arainga, M., Takeda, E., & Aida, Y. (2012). Identification of bovine leukemia virus tax function associated with host cell transcription, signaling, stress response and immune response pathway by microarray-based gene expression analysis. BMC genomics, 13, 121.

Datasheet

SDS

Request for COA

Datasheet Target Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

4B12

Application

WB, IP, IF, ELISA

Immunogen

Amino acids 1-85 mapping at the N-terminus of human Gem.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

Application	Note
ELISA	1:100-1:1,000
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, gelatin

Preservative

Sodium azide

Concentration

0.2 mg/ml

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

GTP binding protein overexpressed in skeletal muscle

Entrez Gene ID

2669

UniProt ID

P55040

Function

Could be a regulatory protein, possibly participating in receptor-mediated signal transduction at the plasma membrane. Has guanine nucleotide-binding activity but undetectable intrinsic GTPase activity.

Biological Process

Cell surface receptor signaling pathway Source: ProtInc
Chromosome organization Source: UniProtKB
Immune response Source: ProtInc
Metaphase plate congression Source: UniProtKB
Mitotic cell cycle Source: UniProtKB
Negative regulation of high voltage-gated calcium channel activity Source: InterPro
Signal transduction Source: ProtInc

Cellular Location

Cell membrane

PTM

Phosphorylated on tyrosine residues.

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Mouse Anti-GEM Recombinant Antibody (4B12)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Mouse Anti-GEM Recombinant Antibody (4B12) (CAT#: CBMAB-G2791-LY)

Related Products

Mouse Anti-GEM Recombinant Antibody (CBLG1-3160) (CAT#: CBMAB-G2792-LY)

Mouse Anti-GEM Recombinant Antibody (4B12) (CAT#: CBMAB-G2791-LY)

Mouse Anti-GEM Recombinant Antibody (CBLG1-3160) (CAT#: V2LY-1225-LY1045)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry