Mouse Anti-GFM2 Recombinant Antibody (CBFYE-1453) (V2LY-1225-LY1113)

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Basic Information

Host Animal
Mouse
Clone
CBFYE-1453
Application
WB, IP, IF, ELISA
Immunogen
Amino acids 161-219 mapping within an internal region of human EF-G2.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
ApplicationNote
ELISA1:100-1:1,000
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, gelatin
Preservative
Sodium azide
Concentration
0.2 mg/ml
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
More Infomation

Target

Full Name
G Elongation Factor Mitochondrial 2
Entrez Gene ID
Human84340
Mouse320806
Rat294672
UniProt ID
HumanQ969S9
MouseQ8R2Q4
RatQ5BJP6
Function
Mitochondrial GTPase that mediates the disassembly of ribosomes from messenger RNA at the termination of mitochondrial protein biosynthesis. Acts in collaboration with MRRF. GTP hydrolysis follows the ribosome disassembly and probably occurs on the ribosome large subunit. Not involved in the GTP-dependent ribosomal translocation step during translation elongation.
Biological Process
Mitochondrial translation Source: UniProtKB
Mitochondrial translational termination Source: Reactome
Ribosome disassembly Source: UniProtKB
Translational elongation Source: GOC
Cellular Location
Mitochondrion
Involvement in disease
Combined oxidative phosphorylation deficiency 39 (COXPD39):
An autosomal recessive disorder due to mitochondrial dysfunction and characterized by global developmental delay, axial hypotonia, dystonia, dysarthria, impaired intellectual development with poor speech, and deficiencies of the mitochondrial respiratory chain enzyme complexes. Neuroimaging shows abnormalities in the putamen and caudate nuclei, along with subcortical white matter involvement.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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