Rat Anti-GFP Recombinant Antibody (230) (CBMAB-G3040-LY)

Name: Rat Anti-GFP Recombinant Antibody (230)
SKU: CBMAB-G3040-LY
Rating: 5 (1 reviews)

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Published Data

Rat Anti-GFP Recombinant Antibody (230) (CBMAB-G3040-LY) in IF

Immuno-gold electron microscopy images of microglia-derived EV containing mE-CD9 fusion protein (GFP+ 10 nm immuno-gold dots) and p-tau (AT8+ 5 nm immuno-gold dots). ...View More

Clayton, K., Delpech, J. C., Herron, S., Iwahara, N., Ericsson, M., Saito, T., Saido, T. C., Ikezu, S., & Ikezu, T. (2021). Plaque associated microglia hyper-secrete extracellular vesicles and accelerate tau propagation in a humanized APP mouse model. Molecular neurodegeneration, 16(1), 18.

Rat Anti-GFP Recombinant Antibody (230) (CBMAB-G3040-LY) in WB and IP

Immunoprecipitation (IP) and western blot (Wb) using specific antibodies against GFP show specific 70 KDa band for fusion proteins in lysates from transgenic fishes Tg(mH2A1:GFP-mH2A1.1) and Tg(mH2A2:GFP-mH2A2) at selected development stages, and 40KDa band for GFP-expressing transgenic fish Tg(H2A:GFP). Wild type-fish lysates were used as negative controls. ...View More

Gonzalez-Munoz, E., Arboleda-Estudillo, Y., Chanumolu, S. K., Otu, H. H., & Cibelli, J. B. (2019). Zebrafish macroH2A variants have distinct embryo localization and function. Scientific reports, 9(1), 8632.

Rat Anti-GFP Recombinant Antibody (230) (CBMAB-G3040-LY) in IF

mH2A isoforms differential colocalization with heterochromatin and mitotic marks in zebrafish embryos at 24 hpf stage. Transgenic zf embryos expressing mH2A1:GFP-mH2A1.1 (A,B,E) or mH2A2:GFP-mH2A2 (C,D,F) at 24 hpf stage were analysed using immunohistochemistry to detect heterochromatin markers trimethyl Histone3 lysine K27 and K9 (H3K27me3 and H3K9me3) (A-D) or mitotic nuclei Phosphohistone H3 (pHH3) (E,F). DAPI was used for nucleus labelling. Confocal microscope image projection of 24 hpf transgenic fish. (A,B) mH2A1:GFP-mH2A1.1 embryo shows YSL localization of GFP positive cells and lack of colocalization with H3K27me3 (A,B) and H3K9m3 (C) throughout the embryo body. (C,D) mH2A2:GFP-mH2A2 embryo stained with heterochromatin markers H3K27me3 (D) and H3K9me3 (E) reveals areas of colocalization. (E,F) mH2A1:GFP-mH2A1.1 (E) or mH2A2:GFP-mH2A2 (F) with labelled pHH3 mitotic nuclei shows partial colocalization of GFP-mH2A2 isoform with mitotic nuclei. Each labelling assay was conducted in triplicate with n = 40-50 embryos/immunolabeling. ...View More

Rat Anti-GFP Recombinant Antibody (230) (CBMAB-G3040-LY) in IF

Nkx2-5 and Isl1 enhancers mark distinct cardiac progenitor populations.
(A) Experimental design of the ex ovo electroporation technique. An electric field is used to introduce different plasmids into the embryo at gastrulation (St. 3), followed by EC culture and imaging. (B-E) Images taken from a 24 hr time-lapse video of an embryo co-electroporated with the Nkx2-5 (GFP) and Isl1 (RFP) enhancers from St. 7 to St. 11. Higher magnification panels (B'''-E''') of the dotted square boxes highlight the differential Nkx2-5/Isl1 enhancers expression. Green arrow: Nkx2-5-en+ cells; Red arrow: Isl1-en+ cells; Yellow arrow: Nkx2-5-en+/Isl1-en+ cells. CC: cardiac crescent; LPM: lateral plate mesoderm; HT: heart tube; smt: somites. Scale bar: 100 µm. ...View More

Zamir, L., Singh, R., Nathan, E., Patrick, R., Yifa, O., Yahalom-Ronen, Y., Arraf, A. A., Schultheiss, T. M., Suo, S., Han, J. J., Peng, G., Jing, N., Wang, Y., Palpant, N., Tam, P. P., Harvey, R. P., & Tzahor, E. (2017). Nkx2.5 marks angioblasts that contribute to hemogenic endothelium of the endocardium and dorsal aorta. eLife, 6, e20994.

Datasheet

SDS

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Datasheet Target Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rat

Clone

230

Application

WB, IP, IF, ELISA

Immunogen

Recombinant GFP.

Specificity

Jellyfish

Antibody Isotype

IgG1

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
ELISA	1:100-1:1,000
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, gelatin

Preservative

Sodium azide

Concentration

0.2 mg/ml

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Green fluorescent protein

Function

Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca2+-activated photoprotein aequorin.

Biological Process

Bioluminescence
Generation of precursor metabolites and energy

PTM

Contains a chromophore consisting of modified amino acid residues. The chromophore is formed by autocatalytic backbone condensation between Ser-65 and Gly-67, and oxidation of Tyr-66 to didehydrotyrosine. Maturation of the chromophore requires nothing other than molecular oxygen.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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