Mouse Anti-GLG1 Recombinant Antibody (1E6) (CBMAB-0410-YC)

Name: Mouse Anti-GLG1 Recombinant Antibody (1E6)
SKU: CBMAB-0410-YC
Rating: 5 (1 reviews)

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Datasheet

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Basic Information

Host Animal

Mouse

Clone

1E6

Application

IHC

Immunogen

Recombinant full-length human GLG1 protein.

Specificity

Human

Antibody Isotype

IgG2b, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
IHC-P	0.5-1 µg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, BSA

Preservative

Sodium azide

Concentration

0.2 mg/ml

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Golgi Glycoprotein 1

Introduction

GLI1 (GLI family zinc finger 1) is transcription factor that is activated by the sonic hedgehog signal transduction cascade and regulates stem cell proliferation. GLI1 belongs to a member of the Kruppel family of zinc finger proteins. The activity and nuclear localization of this protein is negatively regulated by p53 in an inhibitory loop.

Entrez Gene ID

2734

UniProt ID

Q92896

Alternative Names

GLI Family Zinc Finger 1; Glioma-Associated Oncogene Homolog 1 (Zinc Finger Protein); Oncogene GLI; GLI; Glioma-Associated Oncogene Family Zinc Finger 1; GLI-Kruppel Family Member GLI1; Glioma-Associated Oncogene 1; Glioma-Associated Oncogene

Function

Binds fibroblast growth factor and E-selectin (cell-adhesion lectin on endothelial cells mediating the binding of neutrophils).

Biological Process

Bone morphogenesis Source: Ensembl
Negative regulation of protein processing Source: Ensembl
Negative regulation of transforming growth factor beta receptor signaling pathway Source: Ensembl
Regulation of chondrocyte differentiation Source: Ensembl

Cellular Location

Golgi apparatus membrane; Golgi outpost; Microtubule organizing center. Golgi medial cisternae. Localizes to the postsynaptic Golgi apparatus region, also named Golgi outpost, which shapes dendrite morphology by functioning as sites of acentrosomal microtubule nucleation.

Topology

Extracellular: 30-1145
Helical: 1146-1166
Cytoplasmic: 1167-1179

PTM

Fucosylation is essential for binding to E-selectin.By similarity
N-glycosylated. Contains sialic acid residues.By similarity

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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