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Mouse Anti-GLI1 Antibody (1E6) (CBMAB-0410-YC)

Provided herein are mouse monoclonal antibodies against Human GLI1. The antibody clone 1E6 can be used for immunoassay techniques, such as IP and MA.
See all GLI1 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
1E6
Antibody Isotype
IgG2b
Application
IP, MA

Basic Information

Immunogen
Recombinant protein
Specificity
Human
Antibody Isotype
IgG2b
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Supernatant
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
GLI1
Introduction
GLI1 (GLI family zinc finger 1) is transcription factor that is activated by the sonic hedgehog signal transduction cascade and regulates stem cell proliferation. GLI1 belongs to a member of the Kruppel family of zinc finger proteins. The activity and nuclear localization of this protein is negatively regulated by p53 in an inhibitory loop.
Entrez Gene ID
2735
UniProt ID
P08151
Alternative Names
GLI Family Zinc Finger 1; Glioma-Associated Oncogene Homolog 1 (Zinc Finger Protein); Oncogene GLI; GLI; Glioma-Associated Oncogene Family Zinc Finger 1; GLI-Kruppel Family Member GLI1; Glioma-Associated Oncogene 1; Glioma-Associated Oncogene
Function
Acts as a transcriptional activator (PubMed:19706761, PubMed:10806483, PubMed:19878745, PubMed:24076122, PubMed:24311597, PubMed:24217340).

Binds to the DNA consensus sequence 5'-GACCACCCA-3' (PubMed:2105456, PubMed:8378770, PubMed:24217340).

Regulates the transcription of specific genes during normal development (PubMed:19706761).

Plays a role in craniofacial development and digital development, as well as development of the central nervous system and gastrointestinal tract. Mediates SHH signaling (PubMed:19706761, PubMed:28973407).

Plays a role in cell proliferation and differentiation via its role in SHH signaling (PubMed:11238441, PubMed:28973407).

Isoform 2:
Acts as a transcriptional activator, but activates a different set of genes than isoform 1. Activates expression of CD24, unlike isoform 1. Mediates SHH signaling. Promotes cancer cell migration.
Biological Process
Canonical Wnt signaling pathway Source: Ensembl
Cerebellar cortex morphogenesis Source: Ensembl
Digestive tract morphogenesis Source: BHF-UCL
Dorsal/ventral pattern formation Source: Ensembl
Epidermal cell differentiation Source: UniProtKB
Liver regeneration Source: Ensembl
Lung development Source: Ensembl
Negative regulation of canonical Wnt signaling pathway Source: UniProtKB
Notochord regression Source: Ensembl
Osteoblast differentiation Source: UniProtKB
Pituitary gland development Source: Ensembl
Positive regulation of cardiac muscle cell proliferation Source: BHF-UCL
Positive regulation of cell cycle G1/S phase transition Source: BHF-UCL
Positive regulation of cell migration Source: UniProtKB
Positive regulation of cell population proliferation Source: UniProtKB
Positive regulation of DNA replication Source: UniProtKB
Positive regulation of smoothened signaling pathway Source: UniProtKB
Positive regulation of transcription, DNA-templated Source: UniProtKB
Positive regulation of transcription by RNA polymerase II Source: UniProtKB
Prostate gland development Source: Ensembl
Proximal/distal pattern formation Source: Ensembl
Regulation of hepatocyte proliferation Source: Ensembl
Regulation of osteoblast differentiation Source: Ensembl
Regulation of smoothened signaling pathway Source: UniProtKB
Regulation of transcription by RNA polymerase II Source: GO_Central
Response to wounding Source: Ensembl
Smoothened signaling pathway Source: UniProtKB
Smoothened signaling pathway involved in regulation of cerebellar granule cell precursor cell proliferation Source: Ensembl
Spermatogenesis Source: Ensembl
Ventral midline development Source: Ensembl
Cellular Location
Nucleus; Cytoplasm. Tethered in the cytoplasm by binding to SUFU (PubMed:10806483). Activation and translocation to the nucleus is promoted by interaction with STK36 (PubMed:10806483). Phosphorylation by ULK3 may promote nuclear localization (PubMed:19878745). Translocation to the nucleus is promoted by interaction with ZIC1 (PubMed:11238441).
Isoform 2: Nucleus; Cytoplasm
Involvement in disease
Polydactyly, postaxial, A8 (PAPA8):
A form of postaxial polydactyly, a condition characterized by the occurrence of supernumerary digits in the upper and/or lower extremities. In postaxial polydactyly type A, the extra digit is well-formed and articulates with the fifth or a sixth metacarpal/metatarsal. PAPA8 is an autosomal recessive condition characterized by the presence of postaxial extra digits (hexadactyly) on the hands and/or the feet.
Polydactyly, preaxial 1 (PPD1):
A form of polydactyly, a condition defined by the occurrence of supernumerary digits in the upper and/or lower extremities. Preaxial or radial polydactyly refers to the presence of extra digits on the radial side of the hand. PPD1 is an autosomal recessive form characterized by duplication of the distal phalanx of the thumb.
PTM
Phosphorylated in vitro by ULK3.
Acetylation at Lys-518 down-regulates transcriptional activity. Deacetylated by HDAC1.
Ubiquitinated by the CRL2(FEM1B) complex, suppressing GLI1 transcriptional activator activity.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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