Mouse Anti-GLO1 Recombinant Antibody (CBLG1-1277) (V2LY-1225-LY1492)

Name: Mouse Anti-GLO1 Recombinant Antibody (CBLG1-1277)
SKU: V2LY-1225-LY1492
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-GLO1 Recombinant Antibody (CBLG1-1277) (V2LY-1225-LY1492) in IHC

(A1-B2) Representative Glo-I-DAB-stained bright-field images of stacked whole pancreata. CN treatment resulted in increased staining intensity (A1,B1). Staining controls lacking the primary antibody confirm specific Glo-I staining (A2,B2). ...View More

Hollenbach, M., Sonnenberg, S., Sommerer, I., Lorenz, J., & Hoffmeister, A. (2021). Glyoxalase-I Is Upregulated in Acute Cerulein-Induced Pancreatitis: A New Mechanism in Pancreatic Inflammation?. Antioxidants (Basel, Switzerland), 10(10), 1574.

Mouse Anti-GLO1 Recombinant Antibody (CBLG1-1277) (V2LY-1225-LY1492) in WB

(A1,A2) Effects of EP and BrBz on CN-induced expression of Glo-I, NF-κB and RAGE. AR42J cells were pretreated with dexa for 48 h. Another treatment with dexa and 15 mM of EP or 10 µM of BrBz with CN showed no effects after 1 or 6 h of incubation. After 24 h, both EP and BrBz significantly reduced the expression of Glo-I, NF-κB and RAGE. Representative images are shown in (A1) and quantifications in (A2). ...View More

Mouse Anti-GLO1 Recombinant Antibody (CBLG1-1277) (V2LY-1225-LY1492) in WB

WB analysis (A1) indicated reduction of Glo-I on protein levels, quantification showed significant reduction after 24 hours and highest reduction after 72 hours of incubation (A2). ...View More

Hollenbach, M., Sonnenberg, S., Sommerer, I., Lorenz, J., & Hoffmeister, A. (2021). Pitfalls in AR42J-model of cerulein-induced acute pancreatitis. PloS one, 16(1), e0242706.

Mouse Anti-GLO1 Recombinant Antibody (CBLG1-1277) (V2LY-1225-LY1492) in WB

Effect of CA on decreasing glyoxalase-1 (Glo-1) protein expression and MG enzyme activity. Protein expression of Glo-1 in INS-1 cells treated with (A) 2.0 mM MG for 2-8 h and (B) pre-treated with CA, NAC, and medium for 48 h and then treated with 2.0 mM MG for 8 h. (C) The effect of CA on Glo-1 enzyme activity; (D) nuclear factor erythroid 2-related factor 2 (Nrf-2) protein expression in INS-1 cells treated with 2.0 mM MG for 8 h following pre-treatment with CA, NAC, and medium for 48 h. Glo-1 activity is represented as the production of S-d-lactolyglutathione per mg of protein in 1 min. The data are expressed as mean ± S.D. values of three independent experiments. Different letters indicate significant differences at p < 0.05 according to Tukey's multiple range test. Differences between means that share a letter are not statistically significant. ...View More

Yoo, H. J., Hong, C. O., Ha, S. K., & Lee, K. W. (2020). Chebulic Acid Prevents Methylglyoxal-Induced Mitochondrial Dysfunction in INS-1 Pancreatic β-Cells. Antioxidants (Basel, Switzerland), 9(9), 771.

Datasheet

SDS

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Datasheet Target Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBLG1-1277

Application

WB, IP, IF, ELISA, IHC-P

Immunogen

Full-length of human Glyoxalase I.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG2b, κ

Clonality

Monoclonal

Application Notes

Application	Note
ELISA	1:100-1:1,000
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
IHC-P	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, gelatin

Preservative

Sodium azide

Concentration

0.2 mg/ml

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Glyoxalase I

Entrez Gene ID

Human	2739
Mouse	109801
Rat	294320

UniProt ID

Human	Q04760
Mouse	Q9CPU0
Rat	Q6P7Q4

Function

Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione. Involved in the regulation of TNF-induced transcriptional activity of NF-kappa-B. Required for normal osteoclastogenesis.

Biological Process

Carbohydrate metabolic process Source: ProtInc
Glutathione metabolic process Source: Ensembl
Methylglyoxal metabolic process Source: Ensembl
Negative regulation of apoptotic process Source: UniProtKB
Osteoclast differentiation Source: UniProtKB
Regulation of transcription by RNA polymerase II Source: Ensembl

Cellular Location

Cytosol; Extracellular exosome; Nucleoplasm; Plasma membrane; Cytoplasm

PTM

Glutathionylation at Cys-139 inhibits enzyme activity.
Phosphorylated at Thr-107 in the presence of CaMK2. However, this is a consensus site for phosphorylation by CK2 so phosphorylation may be mediated by CK2 rather than CaMK2. Phosphorylation is induced by TNF and suppresses the TNF-induced transcriptional activity of NF-kappa-B.
Exists in a nitric oxide (NO)-modified form. The exact nature of the modification is unknown, but it suppresses the TNF-induced transcriptional activity of NF-kappa-B.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Mouse Anti-GLO1 Recombinant Antibody (CBLG1-1277) (CAT#: CBMAB-G3819-LY)

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Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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