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Mouse Anti-GPC3 Recombinant Antibody (CBT4263) (V2LY-0625-LY1555)


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Tested Data
Mouse Anti-GPC3 Recombinant Antibody (CBT4263) (V2LY-0625-LY1555) in ELISA
Mouse Anti-GPC3 Recombinant Antibody (CBT4263) (V2LY-0625-LY1555) in ELISA
Mouse Anti-GPC3 Recombinant Antibody (CBT4263) (V2LY-0625-LY1555) in WB
Mouse Anti-GPC3 Recombinant Antibody (CBT4263) (V2LY-0625-LY1555) in WB
Mouse Anti-GPC3 Recombinant Antibody (CBT4263) (V2LY-0625-LY1555) in WB
Mouse Anti-GPC3 Recombinant Antibody (CBT4263) (V2LY-0625-LY1555) in WB
Mouse Anti-GPC3 Recombinant Antibody (CBT4263) (V2LY-0625-LY1555) in WB
Mouse Anti-GPC3 Recombinant Antibody (CBT4263) (V2LY-0625-LY1555) in WB
Mouse Anti-GPC3 Recombinant Antibody (CBT4263) (V2LY-0625-LY1555) in IHC
Mouse Anti-GPC3 Recombinant Antibody (CBT4263) (V2LY-0625-LY1555) in IHC
Mouse Anti-GPC3 Recombinant Antibody (CBT4263) (V2LY-0625-LY1555) in FC
Mouse Anti-GPC3 Recombinant Antibody (CBT4263) (V2LY-0625-LY1555) in FC

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBT4263
Antibody Isotype
IgG1
Application
WB, IHC, FC

Basic Information

Immunogen
Purified recombinant fragment of human GPC3 (AA: 55-200) expressed in E. Coli.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal Antibody
Application Notes
ApplicationNote
WB1:500-1:2,000
IHC-P1:200-1:1,000
FC1:200-1:400
ELISA1:10,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS
Preservative
Sodium azide
Concentration
Batch dependent
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Entrez Gene ID
2719
UniProt ID
P51654
Function
Cell surface proteoglycan that bears heparan sulfate (PubMed:14610063).

Negatively regulates the hedgehog signaling pathway when attached via the GPI-anchor to the cell surface by competing with the hedgehog receptor PTC1 for binding to hedgehog proteins (By similarity).

Binding to the hedgehog protein SHH triggers internalization of the complex by endocytosis and its subsequent lysosomal degradation (By similarity).

Positively regulates the canonical Wnt signaling pathway by binding to the Wnt receptor Frizzled and stimulating the binding of the Frizzled receptor to Wnt ligands (PubMed:16227623, PubMed:24496449).

Positively regulates the non-canonical Wnt signaling pathway (By similarity).

Binds to CD81 which decreases the availability of free CD81 for binding to the transcriptional repressor HHEX, resulting in nuclear translocation of HHEX and transcriptional repression (By similarity).

Inhibits the dipeptidyl peptidase activity of DPP4 (PubMed:17549790).

Plays a role in limb patterning and skeletal development by controlling the cellular response to BMP4 (By similarity).

Modulates the effects of growth factors BMP2, BMP7 and FGF7 on renal branching morphogenesis (By similarity).

Required for coronary vascular development (By similarity).

Plays a role in regulating cell movements during gastrulation (By similarity).
Biological Process
Anatomical structure morphogenesis Source: ProtInc
Anterior/posterior axis specification Source: Ensembl
Body morphogenesis Source: Ensembl
Bone mineralization Source: Ensembl
Branching involved in ureteric bud morphogenesis Source: Ensembl
Cell migration Source: GO_Central
Cell migration involved in gastrulation Source: UniProtKB
Cell proliferation involved in kidney development Source: UniProtKB
Cell proliferation involved in metanephros development Source: Ensembl
Coronary vasculature development Source: UniProtKB
Embryonic hindlimb morphogenesis Source: Ensembl
Lung development Source: Ensembl
Mesenchymal cell proliferation involved in ureteric bud development Source: UniProtKB
Mesonephric duct morphogenesis Source: UniProtKB
Negative regulation of canonical Wnt signaling pathway Source: Ensembl
Negative regulation of epithelial cell proliferation Source: Ensembl
Negative regulation of growth Source: Ensembl
Negative regulation of peptidase activity Source: UniProtKB-KW
Negative regulation of smoothened signaling pathway Source: UniProtKB
Osteoclast differentiation Source: Ensembl
Positive regulation of BMP signaling pathway Source: Ensembl
Positive regulation of canonical Wnt signaling pathway Source: UniProtKB
Positive regulation of endocytosis Source: UniProtKB
Positive regulation of glucose import Source: Ensembl
Positive regulation of protein catabolic process Source: UniProtKB
Positive regulation of smoothened signaling pathway Source: Ensembl
Positive regulation of Wnt signaling pathway, planar cell polarity pathway Source: Ensembl
Regulation of canonical Wnt signaling pathway Source: UniProtKB
Regulation of non-canonical Wnt signaling pathway Source: UniProtKB
Regulation of protein localization to membrane Source: GO_Central
Response to bacterium Source: Ensembl
Cellular Location
Cell membrane
Involvement in disease
Simpson-Golabi-Behmel syndrome 1 (SGBS1):
A condition characterized by pre- and postnatal overgrowth (gigantism), facial dysmorphism and a variety of inconstant visceral and skeletal malformations. Characteristic dysmorphic features include macrocephaly with coarse, distinctive facies with a large protruding jaw, broad nasal bridge and cleft palate. Cardiac defects are frequent.
PTM
O-glycosylated; contains heparan sulfate.
Cleaved intracellularly by a furin-like convertase to generate 2 subunits, alpha and beta, which remain associated through disulfide bonds and are associated with the cell surface via the GPI-anchor (PubMed:14610063). This processing is essential for its role in inhibition of hedgehog signaling (PubMed:25653284). A second proteolytic event may result in cleavage of the protein on the cell surface, separating it from the GPI-anchor and leading to its shedding from the cell surface (PubMed:14610063).
More Infomation

Makkouk, A., Yang, X. C., Barca, T., Lucas, A., Turkoz, M., Wong, J. T., ... & Herrman, M. (2021). Off-the-shelf Vδ1 gamma delta T cells engineered with glypican-3 (GPC-3)-specific chimeric antigen receptor (CAR) and soluble IL-15 display robust antitumor efficacy against hepatocellular carcinoma. Journal for immunotherapy of cancer, 9(12).

Guereño, M., Pastore, M. D., Lugones, A. C., Cercato, M., Todaro, L., Urtreger, A., & Peters, M. G. (2020). Glypican-3 (GPC3) inhibits metastasis development promoting dormancy in breast cancer cells by p38 MAPK pathway activation. European Journal of Cell Biology, 99(6), 151096.

Liu, S., Wang, M., Zheng, C., Zhong, Q., Shi, Y., & Han, X. (2020). Diagnostic value of serum glypican-3 alone and in combination with AFP as an aid in the diagnosis of liver cancer. Clinical biochemistry, 79, 54-60.

Batra, S. A., Rathi, P., Guo, L., Courtney, A. N., Fleurence, J., Balzeau, J., ... & Heczey, A. (2020). Glypican-3–Specific CAR T Cells Coexpressing IL15 and IL21 Have Superior Expansion and Antitumor Activity against Hepatocellular CarcinomaIL15 and IL21 Armored GPC3-CAR T Cells against Liver Cancer. Cancer immunology research, 8(3), 309-320.

Fu, Y., Urban, D. J., Nani, R. R., Zhang, Y. F., Li, N., Fu, H., ... & Ho, M. (2019). Glypican‐3‐specific antibody drug conjugates targeting hepatocellular carcinoma. Hepatology, 70(2), 563-576.

Nishida, T., & Kataoka, H. (2019). Glypican 3-targeted therapy in hepatocellular carcinoma. Cancers, 11(9), 1339.

Shimizu, Y., Suzuki, T., Yoshikawa, T., Endo, I., & Nakatsura, T. (2019). Next-generation cancer immunotherapy targeting glypican-3. Frontiers in oncology, 9, 248.

Xu, D., Su, C., Sun, L., Gao, Y., & Li, Y. (2019). Performance of serum glypican 3 in diagnosis of hepatocellular carcinoma: a meta-analysis. Annals of Hepatology, 18(1), 58-67.

Liu, H., Yang, C., Lu, W., & Zeng, Y. (2018). Prognostic significance of glypican-3 expression in hepatocellular carcinoma: A meta-analysis. Medicine, 97(4).

Zhou, F., Shang, W., Yu, X., & Tian, J. (2018). Glypican‐3: a promising biomarker for hepatocellular carcinoma diagnosis and treatment. Medicinal research reviews, 38(2), 741-767.

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For research use only. Not intended for any clinical use.

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