Rabbit Anti-HCAR3 Recombinant Antibody (EG1360) (CBMAB-EN1639-LY)

The product is antibody recognizes GPR109. The antibody EG1360 immunoassay techniques such as: WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:10000.
See all HCAR3 antibodies

Datasheet

Summary

Host Animal

Rabbit

Specificity

Human, Mouse, Rat

Clone

EG1360

Antibody Isotype

IgG

Application

WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:10000

Basic Information

Immunogen

The antibody was produced against synthesized peptide derived from internal of human GPR109.

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name

hydroxycarboxylic acid receptor 3

Entrez Gene ID

Human	8843
Rat	369162

UniProt ID

Human

P49019

Alternative Names

HCA3; HM74; PUMAG; Puma-g; GPR109B

Function

Receptor for 3-OH-octanoid acid mediates a negative feedback regulation of adipocyte lipolysis to counteract prolipolytic influences under conditions of physiological or pathological increases in beta-oxidation rates. Acts as a low affinity receptor for nicotinic acid. This pharmacological effect requires nicotinic acid doses that are much higher than those provided by a normal diet.

Biological Process

G protein-coupled receptor signaling pathway Source: ProtInc

Cellular Location

Cell membrane

Topology

Extracellular: 1-28
Helical: 29-50
Cytoplasmic: 51-63
Helical: 64-85
Extracellular: 86-102
Helical: 103-123
Cytoplasmic: 124-142
Helical: 143-163
Extracellular: 164-194
Helical: 195-209
Cytoplasmic: 210-236
Helical: 237-256
Extracellular: 257-273
Helical: 274-298
Cytoplasmic: 299-387

References

Yang, X., Wei, W., Tan, S., Guo, L., Qiao, S., Yao, B., & Wang, Z. (2021). Identification and verification of HCAR3 and INSL5 as new potential therapeutic targets of colorectal cancer. World journal of surgical oncology, 19(1), 248.

Wnorowski, A., Wnorowska, S., Kurzepa, J., & Parada-Turska, J. (2021). Alterations in kynurenine and NAD+ salvage pathways during the successful treatment of inflammatory bowel disease suggest HCAR3 and NNMT as potential drug targets. International Journal of Molecular Sciences, 22(24), 13497.

Kapolka, N. J., & Isom, D. G. (2020). HCAR3: An underexplored metabolite sensor. Nature reviews. Drug discovery, 19(11), 745.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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Datasheet
SDS
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Second antibody and isotype control

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