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Mouse Anti-GPR15 Recombinant Antibody (CBT3054) (V2LY-0625-LY1706)

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Summary

Host Animal
Mouse
Specificity
Human
Clone
CBT3054
Antibody Isotype
IgG2b
Application
ICC, FC

Basic Information

Immunogen
Purified recombinant fragment of human GPR15 (AA: extra mix) expressed in E. Coli.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG2b
Clonality
Monoclonal Antibody
Application Notes
ApplicationNote
WB1:500-1:2,000
ICC1:200-1:1,000
FC1:200-1:400
ELISA1:10,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS
Preservative
Sodium azide
Concentration
Batch dependent
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
G protein-coupled receptor 15
Entrez Gene ID
UniProt ID
Research Area
Signal Transduction
Function
Probable chemokine receptor. Alternative coreceptor with CD4 for HIV-1 infection.
Biological Process
Angiogenesis
G protein-coupled receptor signaling pathway
Symbiont entry into host cell
T cell migration
Cellular Location
Cell membrane ; Multi-pass membrane protein
Topology
Extracellular: 1-33
Helical: 34-54
Cytoplasmic: 55-69
Helical: 70-90
Extracellular: 91-120
Helical: 121-141
Cytoplasmic: 142-149
Helical: 150-170
Extracellular: 171-192
Helical: 193-213
Cytoplasmic: 214-239
Helical: 240-260
Extracellular: 261-284
Helical: 285-305
Cytoplasmic: 306-360
More Infomation

Haase, T., Müller, C., Stoffers, B., Kirn, P., Waldenberger, M., Kaiser, F. J., ... & Zeller, T. (2022). G Protein-Coupled Receptor 15 Expression Is Associated with Myocardial Infarction. International Journal of Molecular Sciences, 24(1), 180.

Furukawa, K., Ling, J., Sun, Y., Lu, Y., Fu, J., Marukuchi, R., & Chiao, P. J. (2022). Recombinant Thrombomodulin Has Anti-tumor Effects and Enhances the Effects of Gemcitabine for Pancreatic Cancer Through G-protein Coupled Receptor 15. Anticancer research, 42(10), 4679-4687.

Deng, Y., Moo, E. V., Almería, C. V. P., Gentry, P. R., Vedel, L., Mathiesen, J. M., & Bräuner‐Osborne, H. (2022). Delineation of the GPR15 receptor‐mediated Gα protein signalling profile in recombinant mammalian cells. Basic & Clinical Pharmacology & Toxicology, 131(2), 104-113.

Bauer, M. (2021). The Role of GPR15 Function in Blood and Vasculature. International Journal of Molecular Sciences, 22(19), 10824.

Sezin, T., Jegodzinski, L., Meyne, L. M., Gupta, Y., Mousavi, S., Ludwig, R. J., ... & Sadik, C. D. (2021). The G protein‐coupled receptor 15 (GPR15) regulates cutaneous immunology by maintaining dendritic epidermal T cells and regulating the skin microbiome. European Journal of Immunology, 51(6), 1390-1398.

Guo, Y., Zhu, Q., Chen, S., Li, Y., Fu, D., Qiao, D., & Ni, C. (2021). Post-transcriptional suppression of G protein-coupled receptor 15 (GPR15) by microRNA-1225 inhibits proliferation, migration, and invasion of human colorectal cancer cells. 3 Biotech, 11(3), 139.

Jegodzinski, L., Sezin, T., Loser, K., Mousavi, S., Zillikens, D., & Sadik, C. D. (2020). The G protein-coupled receptor (GPR) 15 counteracts antibody-mediated skin inflammation. Frontiers in Immunology, 11, 1858.

Takeshita, A., Yasuma, T., Nishihama, K., D’Alessandro-Gabazza, C. N., Toda, M., Totoki, T., ... & Gabazza, E. C. (2020). Thrombomodulin ameliorates transforming growth factor-β1–mediated chronic kidney disease via the G-protein coupled receptor 15/Akt signal pathway. Kidney International, 98(5), 1179-1192.

Wang, Y., Wang, X., Xiong, Y., Li, C. D., Xu, Q., Shen, L., ... & Wei, D. Q. (2019). An integrated pan-cancer analysis and structure-based virtual screening of GPR15. International Journal of Molecular Sciences, 20(24), 6226.

Haase, T., Müller, C., Krause, J., Röthemeier, C., Stenzig, J., Kunze, S., ... & Zeller, T. (2018). Novel DNA methylation sites influence GPR15 expression in relation to smoking. Biomolecules, 8(3), 74.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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