Mouse Anti-GPR27 Recombinant Antibody (EG1399) (CBMAB-EN1678-LY)

Name: Mouse Anti-GPR27 Recombinant Antibody (EG1399)
SKU: CBMAB-EN1678-LY
Rating: 5 (1 reviews)

See all GPR27 antibodies

Compare Online Inquiry

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

EG1399

Application

ELISA, IP, IF, WB

Immunogen

Amino acids 341-356 within a C-terminal cytoplasmic domain of human SREB1.

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG2a, κ

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, gelatin

Preservative

Sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

G Protein-Coupled Receptor 27

Introduction

GPR27 is a member of the G protein-coupled receptors (GPCRs), a large family of receptors that have a similar structure characterized by 7 transmembrane domains. Activation of GPCRs by extracellular stimuli such as neurotransmitters, hormones, or light induces an intracellular signaling cascade mediated by heterotrimeric GTP-binding proteins, or G proteins.

Entrez Gene ID

Human	2850
Mouse	14761
Rat	65275

UniProt ID

Human	Q9NS67
Mouse	O54897
Rat	Q9JJH3

Alternative Names

SREB1

Function

Orphan receptor. Possible candidate for amine-like G-protein coupled receptor.

Biological Process

Positive regulation of insulin secretion involved in cellular response to glucose stimulus Source: Ensembl
Positive regulation of phospholipase C-activating G protein-coupled receptor signaling pathway Source: Ensembl
Signal transduction Source: ProtInc

Cellular Location

Cell membrane

Topology

Extracellular: 1-23
Helical: 24-44
Cytoplasmic: 45-55
Helical: 56-76
Extracellular: 77-97
Helical: 98-118
Cytoplasmic: 119-139
Helical: 140-160
Extracellular: 161-181
Helical: 182-202
Cytoplasmic: 203-285
Helical: 286-306
Extracellular: 307-320
Helical: 321-341
Cytoplasmic: 342-375

Sakai, A., Yasui, T., Watanave, M., Tatsumi, R., Yamamoto, Y., Takano, W., ... & Takeda, S. (2022). Development of novel potent ligands for GPR85, an orphan G protein‐coupled receptor expressed in the brain. Genes to Cells, 27(5), 345-355.

Dolanc, D., Zorec, T. M., Smole, Z., Maver, A., Horvat, A., Pillaiyar, T., ... & Zorec, R. (2022). The activation of GPR27 increases cytosolic L-lactate in 3T3 embryonic cells and astrocytes. Cells, 11(6), 1009.

Qiu, Y., Hou, Y., Zhou, Y., Xu, J., Bykova, M., Leverenz, J. B., ... & Cheng, F. (2022). Comprehensive characterization of multi-omic landscapes between gut-microbiota metabolites and the G-protein-coupled receptors in Alzheimer's disease. bioRxiv, 2022-09.

Pillaiyar, T., Rosato, F., Wozniak, M., Blavier, J., Charles, M., Laschet, C., ... & Hanson, J. (2021). Structure-activity relationships of agonists for the orphan G protein-coupled receptor GPR27. European Journal of Medicinal Chemistry, 225, 113777.

Breton, T. S., Sampson, W. G., Clifford, B., Phaneuf, A. M., Smidt, I., True, T., ... & DiMaggio, M. A. (2021). Characterization of the G protein-coupled receptor family SREB across fish evolution. Scientific Reports, 11(1), 1-17.

Nath, A. K., Ma, J., Chen, Z. Z., Li, Z., del Carmen Vitery, M., Kelley, M. L., ... & Yeh, J. R. J. (2020). Genetic deletion of gpr27 alters acylcarnitine metabolism, insulin sensitivity, and glucose homeostasis in zebrafish. FASEB journal: official publication of the Federation of American Societies for Experimental Biology, 34(1), 1546.

Chopra, D. G., Yiv, N., Hennings, T. G., Zhang, Y., & Ku, G. M. (2020). Deletion of Gpr27 in vivo reduces insulin mRNA but does not result in diabetes. Scientific reports, 10(1), 1-9.

Laschet, C., Dupuis, N., & Hanson, J. (2018). The G protein-coupled receptors deorphanization landscape. Biochemical Pharmacology, 153, 62-74.

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Mouse Anti-GPR27 Recombinant Antibody (EG1399)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry