Mouse Anti-GPR65 Recombinant Antibody (11F1) (CBMAB-G4665-LY)

Name: Mouse Anti-GPR65 Recombinant Antibody (11F1)
SKU: CBMAB-G4665-LY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

11F1

Application

WB, ICC

Immunogen

NS0 mouse myeloma cell line transfected with human TDAG8/GPR65 (Met1-Glu337)

Specificity

Human

Antibody Isotype

IgG2b

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Lyophilized

Buffer

PBS, trehalose

Concentration

0.25 mg/mL

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

G protein-coupled receptor 65

Entrez Gene ID

8477

UniProt ID

Q8IYL9

Alternative Names

TDAG8; hTDAG8

Function

Receptor for the glycosphingolipid psychosine (PSY) and several related glycosphingolipids (PubMed:11309421).

Plays a role in immune response by maintaining lysosome function and supporting phagocytosis-mediated intracellular bacteria clearance (PubMed:27287411).

May have a role in activation-induced cell death or differentiation of T-cells (By similarity).

Biological Process

Actin cytoskeleton reorganization Source: BHF-UCL
Activation of GTPase activity Source: BHF-UCL
Adenylate cyclase-activating G protein-coupled receptor signaling pathway Source: BHF-UCL
Apoptotic process Source: ProtInc
G protein-coupled receptor signaling pathway Source: BHF-UCL
Immune response Source: ProtInc
Multicellular organism development Source: ProtInc
Positive regulation of cytosolic calcium ion concentration involved in phospholipase C-activating G protein-coupled signaling Pathway Source: GO_Central
Positive regulation of Rho protein signal transduction Source: GO_Central
Positive regulation of stress fiber assembly Source: BHF-UCL
Response to acidic pH Source: BHF-UCL

Cellular Location

Cell membrane

Topology

Extracellular: 1-15
Helical: 16-36
Cytoplasmic: 37-50
Helical: 51-71
Extracellular: 72-89
Helical: 90-110
Cytoplasmic: 111-129
Helical: 130-150
Extracellular: 151-180
Helical: 181-201
Cytoplasmic: 202-227
Helical: 228-248
Extracellular: 249-273
Helical: 274-294
Cytoplasmic: 295-337

Mercier, V., Boucher, G., Devost, D., Bourque, K., Alikashani, A., Beauchamp, C., ... & Rioux, J. D. (2022). IBD-associated G protein-coupled receptor 65 variant compromises signalling and impairs key functions involved in inflammation. Cellular Signalling, 93, 110294.

Tcymbarevich, I., Richards, S. M., Russo, G., Kühn-Georgijevic, J., Cosin-Roger, J., Baebler, K., ... & De Vallière, C. (2019). Lack of the pH-sensing receptor TDAG8 [GPR65] in macrophages plays a detrimental role in murine models of inflammatory bowel disease. Journal of Crohn's and Colitis, 13(2), 245-258.

Yuan, Y., Ma, Y., Zhang, X., Han, R., Hu, X., Yang, J., ... & Pan, F. (2019). Genetic polymorphisms of G protein-coupled receptor 65 gene are associated with ankylosing spondylitis in a Chinese Han population: a case-control study. Human immunology, 80(2), 146-150.

Tcymbarevich, I. V., Eloranta, J. J., Rossel, J. B., Obialo, N., Spalinger, M., Cosin-Roger, J., ... & Misselwitz, B. (2019). The impact of the rs8005161 polymorphism on G protein-coupled receptor GPR65 (TDAG8) pH-associated activation in intestinal inflammation. BMC gastroenterology, 19(1), 1-11.

Wang, H. X., Chen, Y. H., Zhou, J. X., Hu, X. Y., Tan, C., Yan, Y., ... & Chen, J. X. (2018). Overexpression of G-protein-coupled receptors 65 in glioblastoma predicts poor patient prognosis. Clinical neurology and neurosurgery, 164, 132-137.

Wirasinha, R. C., Vijayan, D., Smith, N. J., Parnell, G. P., Swarbrick, A., Brink, R., ... & Batten, M. (2018). GPR 65 inhibits experimental autoimmune encephalomyelitis through CD 4+ T cell independent mechanisms that include effects on iNKT cells. Immunology and cell biology, 96(2), 128-136.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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