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Mouse Anti-GPR65 Recombinant Antibody (11F1) (CBMAB-G4665-LY)

This product is antibody recognizes GPR65. The antibody 11F1 immunoassay techniques such as: ELISA, WB.
See all GPR65 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
11F1
Antibody Isotype
IgG2b
Application
WB, ICC

Basic Information

Immunogen
NS0 mouse myeloma cell line transfected with human TDAG8/GPR65 (Met1-Glu337)
Specificity
Human
Antibody Isotype
IgG2b
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Lyophilized
Buffer
PBS, trehalose
Concentration
0.25 mg/mL
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
G protein-coupled receptor 65
Entrez Gene ID
8477
UniProt ID
Q8IYL9
Alternative Names
TDAG8; hTDAG8
Function
Receptor for the glycosphingolipid psychosine (PSY) and several related glycosphingolipids (PubMed:11309421).

Plays a role in immune response by maintaining lysosome function and supporting phagocytosis-mediated intracellular bacteria clearance (PubMed:27287411).

May have a role in activation-induced cell death or differentiation of T-cells (By similarity).
Biological Process
Actin cytoskeleton reorganization Source: BHF-UCL
Activation of GTPase activity Source: BHF-UCL
Adenylate cyclase-activating G protein-coupled receptor signaling pathway Source: BHF-UCL
Apoptotic process Source: ProtInc
G protein-coupled receptor signaling pathway Source: BHF-UCL
Immune response Source: ProtInc
Multicellular organism development Source: ProtInc
Positive regulation of cytosolic calcium ion concentration involved in phospholipase C-activating G protein-coupled signaling Pathway Source: GO_Central
Positive regulation of Rho protein signal transduction Source: GO_Central
Positive regulation of stress fiber assembly Source: BHF-UCL
Response to acidic pH Source: BHF-UCL
Cellular Location
Cell membrane
Topology
Extracellular: 1-15
Helical: 16-36
Cytoplasmic: 37-50
Helical: 51-71
Extracellular: 72-89
Helical: 90-110
Cytoplasmic: 111-129
Helical: 130-150
Extracellular: 151-180
Helical: 181-201
Cytoplasmic: 202-227
Helical: 228-248
Extracellular: 249-273
Helical: 274-294
Cytoplasmic: 295-337

Mercier, V., Boucher, G., Devost, D., Bourque, K., Alikashani, A., Beauchamp, C., ... & Rioux, J. D. (2022). IBD-associated G protein-coupled receptor 65 variant compromises signalling and impairs key functions involved in inflammation. Cellular Signalling, 93, 110294.

Tcymbarevich, I., Richards, S. M., Russo, G., Kühn-Georgijevic, J., Cosin-Roger, J., Baebler, K., ... & De Vallière, C. (2019). Lack of the pH-sensing receptor TDAG8 [GPR65] in macrophages plays a detrimental role in murine models of inflammatory bowel disease. Journal of Crohn's and Colitis, 13(2), 245-258.

Yuan, Y., Ma, Y., Zhang, X., Han, R., Hu, X., Yang, J., ... & Pan, F. (2019). Genetic polymorphisms of G protein-coupled receptor 65 gene are associated with ankylosing spondylitis in a Chinese Han population: a case-control study. Human immunology, 80(2), 146-150.

Tcymbarevich, I. V., Eloranta, J. J., Rossel, J. B., Obialo, N., Spalinger, M., Cosin-Roger, J., ... & Misselwitz, B. (2019). The impact of the rs8005161 polymorphism on G protein-coupled receptor GPR65 (TDAG8) pH-associated activation in intestinal inflammation. BMC gastroenterology, 19(1), 1-11.

Wang, H. X., Chen, Y. H., Zhou, J. X., Hu, X. Y., Tan, C., Yan, Y., ... & Chen, J. X. (2018). Overexpression of G-protein-coupled receptors 65 in glioblastoma predicts poor patient prognosis. Clinical neurology and neurosurgery, 164, 132-137.

Wirasinha, R. C., Vijayan, D., Smith, N. J., Parnell, G. P., Swarbrick, A., Brink, R., ... & Batten, M. (2018). GPR 65 inhibits experimental autoimmune encephalomyelitis through CD 4+ T cell independent mechanisms that include effects on iNKT cells. Immunology and cell biology, 96(2), 128-136.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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