Mouse Anti-GPR68 Recombinant Antibody (15B8) (CBMAB-G4666-LY)

This product is antibody recognizes GPR68. The antibody 15B8 immunoassay techniques such as: ELISA, WB.
See all GPR68 antibodies

Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

15B8

Antibody Isotype

IgG2b, κ

Application

ELISA, WB

Basic Information

Immunogen

Partial peptide of Human GPR68

Specificity

Human

Antibody Isotype

IgG2b, κ

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Concentration

0.25 mg/mL

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name

G PROTEIN-COUPLED RECEPTOR 68

Introduction

The protein encoded by this gene is a G protein-coupled receptor for sphingosylphosphorylcholine. The encoded protein is a proton-sensing receptor, inactive at pH 7.8 but active at pH 6.8. Mutations in this gene are a cause of amelogenesis imperfecta. [provided by RefSeq, Feb 2017]

Entrez Gene ID

8111

UniProt ID

Q15743

Alternative Names

G Protein-Coupled Receptor 68; Sphingosylphosphorylcholine Receptor; GPR12A; OGR1; Ovarian Cancer G Protein-Coupled Receptor; 1; Ovarian Cancer G-Protein Coupled Receptor 1; G-Protein Coupled Receptor 68; AI2A6; OGR-1;

Function

Proton-sensing receptor involved in pH homeostasis. May represents an osteoblastic pH sensor regulating cell-mediated responses to acidosis in bone. Mediates its action by association with G proteins that stimulates inositol phosphate (IP) production or Ca2+ mobilization. The receptor is almost silent at pH 7.8 but fully activated at pH 6.8. Also functions as a metastasis suppressor gene in prostate cancer (By similarity).

Biological Process

Cellular response to pH Source: GO_Central
G protein-coupled receptor signaling pathway Source: ProtInc
Inflammatory response Source: ProtInc
Negative regulation of monocyte differentiation Source: Ensembl
Positive regulation of insulin secretion involved in cellular response to glucose stimulus Source: Ensembl
Positive regulation of osteoclast development Source: Ensembl

Cellular Location

Cell membrane

Involvement in disease

Amelogenesis imperfecta, hypomaturation type, 2A6 (AI2A6):
A defect of enamel formation. The disorder involves both primary and secondary dentitions. The teeth have a shiny agar jelly appearance and the enamel is softer than normal. Brown pigment is present in middle layers of enamel.

Topology

Extracellular: 1-21
Helical: 22-46
Cytoplasmic: 47-58
Helical: 59-80
Extracellular: 81-95
Helical: 96-117
Cytoplasmic: 118-136
Helical: 137-158
Extracellular: 159-183
Helical: 184-205
Cytoplasmic: 206-228
Helical: 229-249
Extracellular: 250-263
Helical: 264-284
Cytoplasmic: 285-365

References

van Setten, G. B. (2023). Expression of GPR-68 in Human Corneal and Conjunctival Epithelium. Possible indicator and mediator of attrition associated inflammation at the ocular surface. Journal Français d'Ophtalmologie, 46(1), 19-24.

van Setten, G. B. (2022). GPR-68 in human lacrimal gland. Detection and possible role in the pathogenesis of dry eye disease. Journal Français d'Ophtalmologie, 45(8), 921-927.

de Vallière, C., Cosin-Roger, J., Baebler, K., Schoepflin, A., Mamie, C., Mollet, M., ... & Rogler, G. (2022). pH-sensing G protein-coupled receptor OGR1 (GPR68) expression and activation increases in intestinal inflammation and fibrosis. International Journal of Molecular Sciences, 23(3), 1419.

He, X., Feng, S., Hawkins, C., Lawley, L., Fan, W., Xu, Y., ... & Fang, J. (2020). G protein-coupled receptor 68 increases the number of B lymphocytes. American Journal of Blood Research, 10(2), 15.

Yu, X., Huang, X. P., Kenakin, T. P., Slocum, S. T., Chen, X., Martini, M. L., ... & Jin, J. (2019). Design, Synthesis, and Characterization of Ogerin-Based Positive Allosteric Modulators for G Protein-Coupled Receptor 68 (GPR68). Journal of medicinal chemistry, 62(16), 7557-7574.

Wiley, S. Z., Sriram, K., Salmerón, C., & Insel, P. A. (2019). GPR68: an emerging drug target in cancer. International journal of molecular sciences, 20(3), 559.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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