Rabbit Anti-GPRC5A Recombinant Antibody (D4S7D) (CBMAB-R0536-CN)

Name: Rabbit Anti-GPRC5A Recombinant Antibody (D4S7D)
SKU: CBMAB-R0536-CN
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

D4S7D

Application

WB, IP, IHC-p, IF

Immunogen

Synthetic peptide corresponding to residues surrounding Val295 of human RAIG1 protein.

Specificity

Human

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer

HEPES, pH 7.5, 100 µg/ml BSA, 50% glycerol

Preservative

0.02% sodium azide

More Infomation

Target

Full Name

G protein-coupled receptor class C group 5 member A

Introduction

This gene encodes a member of the type 3 G protein-coupling receptor family, characterized by the signature 7-transmembrane domain motif. The encoded protein may be involved in interaction between retinoid acid and G protein signalling pathways. Retinoic acid plays a critical role in development, cellular growth, and differentiation. This gene may play a role in embryonic development and epithelial cell differentiation. [provided by RefSeq, Jul 2008]

Entrez Gene ID

9052

UniProt ID

Q8NFJ5

Alternative Names

G Protein-Coupled Receptor Class C Group 5 Member A; G-Protein Coupled Receptor Family C Group 5 Member A; Retinoic Acid-Induced Gene 1 Protein; Phorbol Ester Induced Gene 1; Retinoic Acid Induced 3; GPCR5A; PEIG-1; RAIG1; RAI3;

Function

Orphan receptor. Could be involved in modulating differentiation and maintaining homeostasis of epithelial cells. This retinoic acid-inducible GPCR provide evidence for a possible interaction between retinoid and G-protein signaling pathways. Functions as a negative modulator of EGFR signaling (By similarity).

May act as a lung tumor suppressor (PubMed:18000218).

Biological Process

Negative regulation of epidermal growth factor-activated receptor activity Source: UniProtKB
Signal transduction Source: ProtInc

Cellular Location

Cell membrane; Cytoplasmic vesicle membrane. Localized in perinuclear vesicles, probably Golgi-associated vesicles.

Topology

Extracellular: 1-33
Helical: 34-54
Cytoplasmic: 55-68
Helical: 69-89
Extracellular: 90-97
Helical: 98-118
Cytoplasmic: 119-129
Helical: 130-150
Extracellular: 151-176
Helical: 177-197
Cytoplasmic: 198-212
Helical: 213-233
Extracellular: 234-247
Helical: 248-268
Cytoplasmic: 269-357

PTM

Phosphorylated in two conserved double-tyrosine motifs, TYR-317/TYR-320 and TYR-347/TYR-350, by EGFR; leading to inactivation of the tumor suppressive function of GPRC5A in lung cancer cells. TYR-317 and TYR-320 are the preferred residues responsible for EGFR-mediated GPRC5A phosphorylation.

Wei, R., Qi, G., Zeng, Z., Shen, N., Wang, Z., Shen, H., ... & Wang, C. (2021). IMUP and GPRC5A: two newly identified risk score indicators in pancreatic ductal adenocarcinoma. Cancer Cell International, 21, 1-19.

Moyano‐Galceran, L., Pietilä, E. A., Turunen, S. P., Corvigno, S., Hjerpe, E., Bulanova, D., ... & Lehti, K. (2020). Adaptive RSK‐EphA2‐GPRC5A signaling switch triggers chemotherapy resistance in ovarian cancer. EMBO molecular medicine, 12(4), e11177.

Sawada, Y., Kikugawa, T., Iio, H., Sakakibara, I., Yoshida, S., Ikedo, A., ... & Imai, Y. (2020). GPRC5A facilitates cell proliferation through cell cycle regulation and correlates with bone metastasis in prostate cancer. International Journal of Cancer, 146(5), 1369-1382.

Wang, T., Jing, B., Xu, D., Liao, Y., Song, H., Sun, B., ... & Deng, J. (2020). PTGES/PGE2 signaling links immunosuppression and lung metastasis in Gprc5a-knockout mouse model. Oncogene, 39(15), 3179-3194.

Richter, L., Oberländer, V., & Schmidt, G. (2020). RhoA/C inhibits proliferation by inducing the synthesis of GPRC5A. Scientific Reports, 10(1), 12532.

Greenhough, A., Bagley, C., Heesom, K. J., Gurevich, D. B., Gay, D., Bond, M., ... & Williams, A. C. (2018). Cancer cell adaptation to hypoxia involves a HIF‐GPRC5A‐YAP axis. EMBO molecular medicine, 10(11), e8699.

Gu, C., Zhou, N., Wang, Z., Li, G., Kou, Y., Yu, S., ... & Tian, F. (2018). circGprc5a promoted bladder oncogenesis and metastasis through Gprc5a-targeting peptide. Molecular Therapy-Nucleic Acids, 13, 633-641.

Jiang, X., Xu, X., Wu, M., Guan, Z., Su, X., Chen, S., ... & Teng, L. (2018). GPRC5A: an emerging biomarker in human cancer. BioMed Research International, 2018.

Liu, B., Yang, H., Pilarsky, C., & Weber, G. F. (2018). The effect of GPRC5a on the proliferation, migration ability, chemotherapy resistance, and phosphorylation of GSK-3β in pancreatic cancer. International journal of molecular sciences, 19(7), 1870.

Ma, X., Schwarz, A., Sevilla, S. Z., Levin, A., Hultenby, K., Wernerson, A., ... & Patrakka, J. (2018). Depletion of Gprc5a promotes development of diabetic nephropathy. Journal of the American Society of Nephrology, 29(6), 1679-1689.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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