Rabbit Anti-GSTO1 Recombinant Antibody (
2C7) (V2LY-0725-LY737)

Name: Rabbit Anti-GSTO1 Recombinant Antibody (2C7)
SKU: V2LY-0725-LY737
Rating: 5 (1 reviews)

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Tested Data

Rabbit Anti-GSTO1 Recombinant Antibody (2C7) (V2LY-0725-LY737) in WB

Western Blot
Positive WB detected in: Jurkat whole cell lysate, PC3 whole cell lysate, Raji whole cell lysate, K562 whole cell lysate, Hela whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate
All lanes: GSTO1 antibody at 1.25μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 28, 24, 25 KDa
Observed band size: 28 KDa

Rabbit Anti-GSTO1 Recombinant Antibody (2C7) (V2LY-0725-LY737) in IHC

IHC image of V2LY-0725-LY737 diluted at 1:125 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Rabbit Anti-GSTO1 Recombinant Antibody (2C7) (V2LY-0725-LY737) in IHC

IHC image of V2LY-0725-LY737 diluted at 1:125 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Rabbit Anti-GSTO1 Recombinant Antibody (2C7) (V2LY-0725-LY737) in IF

Immunofluorescence staining of HepG2 cells with V2LY-0725-LY737 at 1:41, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

2C7

Application

ELISA, WB, IHC, IF

Immunogen

A synthesized peptide derived from human GSTO1.

Host Species

Rabbit

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:5,000
IHC	1:50-1:200
IF	1:20-1:200

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, glycerol

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Glutathione S-Transferase Omega 1

Entrez Gene ID

9446

UniProt ID

P78417

Function

Exhibits glutathione-dependent thiol transferase and dehydroascorbate reductase activities. Has S-(phenacyl)glutathione reductase activity. Has also glutathione S-transferase activity. Participates in the biotransformation of inorganic arsenic and reduces monomethylarsonic acid (MMA) and dimethylarsonic acid.

Biological Process

Cellular response to arsenic-containing substance Source: UniProtKB
Glutathione metabolic process Source: GO_Central
L-ascorbic acid metabolic process Source: UniProtKB
Negative regulation of ryanodine-sensitive calcium-release channel activity Source: BHF-UCL
Positive regulation of ryanodine-sensitive calcium-release channel activity Source: BHF-UCL
Positive regulation of skeletal muscle contraction by regulation of release of sequestered calcium ion Source: BHF-UCL
Regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion Source: BHF-UCL
Regulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulum Source: BHF-UCL
Xenobiotic catabolic process Source: UniProtKB

Cellular Location

Cytosol

Djukic, T., Stevanovic, G., Coric, V., Bukumiric, Z., Pljesa-Ercegovac, M., Matic, M., ... & Simic, T. (2022). GSTO1, GSTO2 and ACE2 polymorphisms modify susceptibility to developing COVID-19. Journal of Personalized Medicine, 12(3), 458.

Sobot, V., Stamenkovic, M., Simic, T., Jerotic, D., Djokic, M., Jaksic, V., ... & Djukic, T. (2022). Association of GSTO1, GSTO2, GSTP1, GPX1 and SOD2 polymorphism with primary open angle glaucoma. Experimental Eye Research, 214, 108863.

Paul, S., Bhardwaj, M., & Kang, S. C. (2022). GSTO1 confers drug resistance in HCT‑116 colon cancer cells through an interaction with TNFαIP3/A20. International Journal of Oncology, 61(5), 1-12.

Oakley, A. J. (2022). Proposed mechanism for monomethylarsonate reductase activity of human omega-class glutathione transferase GSTO1-1. Biochemical and Biophysical Research Communications, 590, 7-13.

Sokulsky, L. A., Goggins, B., Sherwin, S., Eyers, F., Kaiko, G. E., Board, P. G., ... & Foster, P. S. (2020). GSTO1‐1 is an upstream suppressor of M2 macrophage skewing and HIF‐1α‐induced eosinophilic airway inflammation. Clinical & Experimental Allergy, 50(5), 609-624.

Saisawang, C., Wongsantichon, J., Robinson, R. C., & Ketterman, A. J. (2019). Glutathione transferase Omega 1‐1 (GSTO1‐1) modulates Akt and MEK1/2 signaling in human neuroblastoma cell SH‐SY5Y. Proteins: Structure, Function, and Bioinformatics, 87(7), 588-595.

Wang, J., Gao, Y., Cheng, X., Yang, J., Zhao, Y., Xu, H., ... & Wang, J. (2019). GSTO1 acts as a mediator in sodium fluoride-induced alterations of learning and memory related factors expressions in the hippocampus cell line. Chemosphere, 226, 201-209.

Radic, T., Coric, V., Bukumiric, Z., Pljesa-Ercegovac, M., Djukic, T., Avramovic, N., ... & Savic-Radojevic, A. (2019). GSTO1* CC Genotype (rs4925) predicts shorter survival in clear cell renal cell carcinoma male patients. Cancers, 11(12), 2038.

Li, L., Zhao, Y., Cao, R., Li, L., Cai, G., Li, J., ... & Zhang, Z. (2019). Activity-based protein profiling reveals GSTO1 as the covalent target of piperlongumine and a promising target for combination therapy for cancer. Chemical Communications, 55(30), 4407-4410.

Fernando, N., Wooff, Y., Aggio-Bruce, R., Chu-Tan, J. A., Jiao, H., Dietrich, C., ... & Natoli, R. (2018). Photoreceptor survival is regulated by GSTO1-1 in the degenerating retina. Investigative ophthalmology & visual science, 59(11), 4362-4374.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Antibody Pairs

GSTO1 Matched Antibody Pair (510) (CAT#: APMAB-510LY)

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Isotype control

For research use only. Not intended for any clinical use.

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