Rabbit Anti-GYPB Recombinant Antibody (CBLG1-3051) (CBMAB-G5857-LY)

Name: Rabbit Anti-GYPB Recombinant Antibody (CBLG1-3051)
SKU: CBMAB-G5857-LY
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

CBLG1-3051

Application

WB, FC, IHC, IF, IP

Immunogen

Human GYPB.

Specificity

Human

Antibody Isotype

IgG

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, BSA, glycerol

Preservative

Sodium azide

Concentration

1 mg/ml

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Glycophorin B (Mns Blood Group)

Introduction

Glycophorins A (GYPA) and B (GYPB) are major sialoglycoproteins of the human erythrocyte membrane which bear the antigenic determinants for the MN and Ss blood groups. GYPB gene consists of 5 exons and has 97% sequence homology with GYPA from the 5' UTR to the coding sequence encoding the first 45 amino acids. In addition to the M or N and S or s antigens, that commonly occur in all populations, about 40 related variant phenotypes have been identified. These variants include all the variants of the Miltenberger complex and several isoforms of Sta; also, Dantu, Sat, He, Mg, and deletion variants Ena, S-s-U- and Mk. Most of the variants are the result of gene recombinations between GYPA and GYPB. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2015]

Entrez Gene ID

2994

UniProt ID

P06028

Function

This protein is a minor sialoglycoprotein in erythrocyte membranes.

Cellular Location

Cell membrane

Topology

Extracellular: 20-59
Helical: 60-81
Cytoplasmic: 82-91

PTM

The N-terminal extracellular domain is heavily glycosylated on serine and threonine residues.

Liang, Y., Ren, J., Zhong, F., Hong, W., Su, Y., Wu, F., ... & Xu, Y. (2022). Characterization of alternatively spliced transcript variants of glycophorin A and glycophorin B genes in Chinese blood donors. Vox Sanguinis, 117(5), 715-723.

Amuzu, D. S., Rockett, K. A., Leffler, E. M., Ansah, F., Amoako, N., Morang’a, C. M., ... & Awandare, G. A. (2021). High-throughput genotyping assays for identification of glycophorin B deletion variants in population studies. Experimental Biology and Medicine, 246(8), 916-928.

Wei, L., Sun, A., Wen, J., Wang, Z., Li, Q., Liao, Y., ... & Ji, Y. (2021). Molecular genetic analysis of Mia‐positive hybrid glycophorins revealed two novel alleles of GP. Vw and multiple variant transcripts of GYPB existing in both the homozygous GP. Mur and wild‐type GPB individuals. Transfusion, 61(8), 2477-2486.

Gassner, C., Denomme, G. A., Portmann, C., Bensing, K. M., Mattle-Greminger, M. P., Meyer, S., ... & Frey, B. M. (2020). Two prevalent∼ 100-kb GYPB deletions causative of the GPB-Deficient blood group MNS phenotype S–s–U–in black Africans. Transfusion Medicine and Hemotherapy, 47(4), 326-336.

Tsuneyama, H., Isa, K., Watanabe‐Okochi, N., Ogasawara, K., Uchikawa, M., & Satake, M. (2020). An unusual variant glycophorin expressing protease‐resistant M antigen encoded by the GYPB‐E (2‐4)‐B hybrid gene. Vox Sanguinis, 115(7), 579-585.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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