Rabbit Anti-H1-4 (phosphorylated Thr17) Recombinant Antibody (CBNH-143) (CBMAB-R4468-CN)

Name: Rabbit Anti-H1-4 (phosphorylated Thr17) Recombinant Antibody (CBNH-143)
SKU: CBMAB-R4468-CN
Rating: 5 (1 reviews)

See all H1-4 antibodies

Compare Online Inquiry

Datasheet

SDS

Request for COA

Datasheet Target Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

CBNH-143

Application

ELISA, IHC, IF

Immunogen

A synthesized peptide

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.4, 150 mM Sodium chloride, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

More Infomation

Target

Full Name

H1.4 linker histone, cluster member

Introduction

Histones are basic nuclear proteins responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H1 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. [provided by RefSeq, Aug 2015]

Entrez Gene ID

3008

UniProt ID

P10412

Alternative Names

Histone Cluster 1 H1 Family Member E; H1 Histone Family, Member 4; Histone Cluster 1, H1e; Histone 1, H1e; Histone H1s-4; Histone H1b; H1F4;

Function

Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Also acts as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation (By similarity).

Biological Process

Chromosome condensation
Negative regulation of DNA recombinationSource:GO_Central
Negative regulation of transcription by RNA polymerase IISource:Ensembl
Nucleosome assembly

Cellular Location

Nucleus
Chromosome
Note: Mainly localizes in heterochromatin. Dysplays a punctuate staining pattern in the nucleus.

Involvement in disease

Rahman syndrome (RMNS):
An autosomal dominant syndrome characterized by intellectual disability and overgrowth manifesting as increased birth length, height, weight, and/or head circumference.

PTM

H1 histones are progressively phosphorylated during the cell cycle, becoming maximally phosphorylated during late G2 phase and M phase, and being dephosphorylated sharply thereafter.
Acetylated at Lys-26. Deacetylated at Lys-26 by SIRT1.
Citrullination at Arg-54 (H1R54ci) by PADI4 takes place within the DNA-binding site of H1 and results in its displacement from chromatin and global chromatin decondensation, thereby promoting pluripotency and stem cell maintenance.
ADP-ribosylated on Ser-150 in response to DNA damage.

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Rabbit Anti-H1-4 (phosphorylated Thr17) Recombinant Antibody (CBNH-143)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Related Products

Rabbit Anti-H1-4 Recombinant Antibody (CBFYH-1156) (CAT#: CBMAB-H2110-FY)

Mouse Anti-H1-4 Recombinant Antibody (CBFYH-1155) (CAT#: CBMAB-H2109-FY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry