Mouse Anti-HCRT Recombinant Antibody (CBXO-0559) (CBMAB-O0554-CQ)

Name: Mouse Anti-HCRT Recombinant Antibody (CBXO-0559)
SKU: CBMAB-O0554-CQ
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBXO-0559

Application

IHC

Immunogen

Human Orexin-A peptide

Specificity

Human

Antibody Isotype

IgG1

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Lyophilized

Buffer

PBS

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Hypocretin Neuropeptide Precursor

Introduction

This gene encodes a hypothalamic neuropeptide precursor protein that gives rise to two mature neuropeptides, orexin A and orexin B, by proteolytic processing. Orexin A and orexin B, which bind to orphan G-protein coupled receptors HCRTR1 and HCRTR2, function in the regulation of sleep and arousal. This neuropeptide arrangement may also play a role in feeding behavior, metabolism, and homeostasis.

Entrez Gene ID

3060

UniProt ID

O43612

Alternative Names

Hypocretin Neuropeptide Precursor; Prepro-Orexin; Orexin; PPOX; OX; Hypocretin (Orexin) Neuropeptide Precursor

Function

Neuropeptides that play a significant role in the regulation of food intake and sleep-wakefulness, possibly by coordinating the complex behavioral and physiologic responses of these complementary homeostatic functions. A broader role in the homeostatic regulation of energy metabolism, autonomic function, hormonal balance and the regulation of body fluids, is also suggested. Orexin-A binds to both OX1R and OX2R with a high affinity, whereas orexin-B binds only to OX2R with a similar high affinity.

Biological Process

Chemical synaptic transmission Source: ProtInc
Eating behavior Source: GO_Central
Excitatory postsynaptic potential Source: Ensembl
Negative regulation of DNA replication Source: Ensembl
Negative regulation of potassium ion transport Source: Ensembl
Negative regulation of transmission of nerve impulse Source: Ensembl
Neuropeptide signaling pathway Source: UniProtKB-KW
Phospholipase C-activating G protein-coupled receptor signaling pathway Source: Ensembl
Positive regulation of calcium ion transport Source: Ensembl
Positive regulation of cold-induced thermogenesis Source: YuBioLab
Positive regulation of cytosolic calcium ion concentration Source: Ensembl
Positive regulation of transmission of nerve impulse Source: GO_Central
Protein kinase C-activating G protein-coupled receptor signaling pathway Source: Ensembl
Regulation of neurotransmitter secretion Source: GO_Central
Response to starvation Source: GO_Central
Sleep Source: GO_Central
Temperature homeostasis Source: GO_Central

Cellular Location

Rough endoplasmic reticulum; Cytoplasmic vesicle; Synapse. Associated with perikaryal rough endoplasmic reticulum as well as cytoplasmic large granular vesicles at synapses.

Involvement in disease

Narcolepsy 1 (NRCLP1):
The disease is caused by variants affecting the gene represented in this entry. Human narcolepsy is associated with a deficient orexin system. Orexins are absent and/or greatly diminished in the brain and cerebrospinal fluid (CSF) of most narcoleptic patients. Neurological disabling sleep disorder, characterized by excessive daytime sleepiness, sleep fragmentation, symptoms of abnormal rapid-eye-movement (REM) sleep, cataplexy, hypnagogic hallucinations, and sleep paralysis. Cataplexy is a sudden loss of muscle tone triggered by emotions, which is the most valuable clinical feature used to diagnose narcolepsy. Human narcolepsy is primarily a sporadically occurring disorder but familial clustering has been observed.

PTM

Specific enzymatic cleavages at paired basic residues yield the different active peptides.

Holm, A., Possovre, M. L., Bandarabadi, M., Moseholm, K. F., Justinussen, J. L., Bozic, I., ... & Kornum, B. R. (2022). The evolutionarily conserved miRNA-137 targets the neuropeptide hypocretin/orexin and modulates the wake to sleep ratio. Proceedings of the National Academy of Sciences, 119(17), e2112225119.

Misawa, K., Mima, M., Imai, A., Mochizuki, D., Misawa, Y., Endo, S., ... & Mineta, H. (2018). The neuropeptide genes SST, TAC1, HCRT, NPY, and GAL are powerful epigenetic biomarkers in head and neck cancer: a site-specific analysis. Clinical epigenetics, 10(1), 1-10.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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