Mouse Anti-HECTD2 Recombinant Antibody (3D6) (CBMAB-A3816-LY)

The product is antibody recognizes HECTD2. The antibody 3D6 immunoassay techniques such as: WB, ELISA.
See all HECTD2 antibodies

Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

3D6

Antibody Isotype

IgG2a, κ

Application

WB, ELISA

Basic Information

Immunogen

HECTD2 (NP_877497, 331 a.a. ~ 440 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name

HECT domain containing 2

Entrez Gene ID

143279

UniProt ID

Q5U5R9

Alternative Names

FLJ16050

Function

E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates.

(Microbial infection) Catalyzes ubiquitination of Botulinum neurotoxin A light chain (LC) of C.botulinum neurotoxin type A (BoNT/A).

Cellular Location

Cytosol

References

Ryu, T. Y., Kim, K., Han, T. S., Lee, M. O., Lee, J., Choi, J., ... & Cho, H. S. (2022). Human gut-microbiome-derived propionate coordinates proteasomal degradation via HECTD2 upregulation to target EHMT2 in colorectal cancer. The ISME Journal, 16(5), 1205-1221.

Ma, L., Li, D. H., & Xu, Z. (2022). HECTD2 Represses Cell Proliferation in Colorectal Cancer through Driving Ubiquitination and Degradation of LPCAT1. Molecular Biology, 56(4), 533-542.

Ottina, E., Panova, V., Doglio, L., Kazachenka, A., Cornish, G., Kirkpatrick, J., ... & Kassiotis, G. (2021). E3 ubiquitin ligase HECTD2 mediates melanoma progression and immune evasion. Oncogene, 40(37), 5567-5578.

Lv, D., Shen, T., Yao, J., Yang, Q., Xiang, Y., & Ma, Z. (2021). HIF-1α induces HECTD2 up-regulation and aggravates the malignant progression of renal cell cancer via repressing miR-320a. Frontiers in Cell and Developmental Biology, 9, 775642.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Related Products

Mouse Anti-HECTD2 (AA 331-441) Recombinant Antibody (CBFYH-0917)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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SDS
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