Mouse Anti-HEPACAM Recombinant Antibody (CBFYM-0454) (CBMAB-M0572-FY)

Name: Mouse Anti-HEPACAM Recombinant Antibody (CBFYM-0454)
SKU: CBMAB-M0572-FY
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBFYM-0454

Application

ICC, IHC, WB

Specificity

Human, Rat, Mouse

Antibody Isotype

IgG2b

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Hepatic And Glial Cell Adhesion Molecule

Introduction

The protein encoded by this gene is a single-pass type I membrane protein that localizes to the cytoplasmic side of the cell membrane. The encoded protein acts as a homodimer and is involved in cell motility and cell-matrix interactions. The expression of this gene is downregulated or undetectable in many cancer cell lines, so this may be a tumor suppressor gene.

Entrez Gene ID

Human	220296
Mouse	72927
Rat	300517

UniProt ID

Human	Q14CZ8
Mouse	Q640R3
Rat	D3ZEI4

Alternative Names

Hepatic And Glial Cell Adhesion Molecule; Hepatocyte Cell Adhesion Molecule; Protein HepaCAM; Glial Cell Adhesion Molecule; GlialCAM; MLC2A; MLC2B

Function

Involved in regulating cell motility and cell-matrix interactions. May inhibit cell growth through suppression of cell proliferation.

Biological Process

Cell adhesion Source: UniProtKB-KW
Cell cycle Source: UniProtKB-KW
Cellular protein localization Source: Ensembl
Regulation of growth Source: UniProtKB-KW

Cellular Location

Cytoplasm; Membrane. In MCF-7 breast carcinoma and hepatic Hep 3B2.1-7 and Hep-G2 cell lines, localization of HEPACAM is cell density-dependent. In well spread cells, localized to punctate structures in the perinuclear membrane, cytoplasm, and at cell surface of protusions. In confluent cells, localized predominantly to the cytoplasmic membrane, particularly in areas of cell-cell contacts. Colocalizes with CDH1.

Involvement in disease

Leukoencephalopathy, megalencephalic, with subcortical cysts, 2A (MLC2A):
A neurodegenerative disorder characterized by infantile-onset macrocephaly and later onset of motor deterioration, with ataxia and spasticity, seizures, and cognitive decline of variable severity. The brain appears swollen on magnetic resonance imaging with white-matter abnormalities and subcortical cysts, in all stages of the disease.
Leukoencephalopathy, megalencephalic, with subcortical cysts, 2B (MLC2B):
A neurodegenerative disorder characterized by infantile-onset of macrocephaly and mildly delayed motor development associated with white-matter abnormalities on brain magnetic resonance imaging. The phenotype is milder that MLC2A, with better preserved cerebellar white matter and no subcortical cysts outside the temporal region. On follow-up, patients show normal or almost normal motor function. Some patients have normal intelligence, whereas others have a significant cognitive deficiency.

Topology

Extracellular: 34-240
Helical: 241-261
Cytoplasmic: 262-416

PTM

N-glycosylated.

Chen, E., Liu, N., Zhao, Y., Tang, M., Ou, L., Wu, X., & Luo, C. (2022). Panobinostat reverses HepaCAM gene expression and suppresses proliferation by increasing histone acetylation in prostate cancer. Gene, 808, 145977.

He, Z., Gao, Y., Li, T., Yu, C., Ou, L., & Luo, C. (2022). HepaCAM‑PIK3CA axis regulates the reprogramming of glutamine metabolism to inhibit prostate cancer cell proliferation. International Journal of Oncology, 60(4), 1-17.

De, A., Lattier, J. M., Morales, J. E., Kelly, J. R., Zheng, X., Chen, Z., ... & McCarty, J. H. (2022). HepaCAM Suppresses Glioblastoma Stem Cell Invasion in the Brain. bioRxiv, 2022-08.

Baldwin, K. T., Tan, C. X., Strader, S. T., Jiang, C., Savage, J. T., Elorza-Vidal, X., ... & Eroglu, C. (2021). HepaCAM controls astrocyte self-organization and coupling. Neuron, 109(15), 2427-2442.

Li, T., Liu, N., Gao, Y., Quan, Z., Hao, Y., Yu, C., ... & Wu, X. (2021). Long noncoding RNA HOTAIR regulates the invasion and metastasis of prostate cancer by targeting hepaCAM. British journal of cancer, 124(1), 247-258.

Sofroniew, M. V. (2021). HepaCAM shapes astrocyte territories, stabilizes gap-junction coupling, and influences neuronal excitability. Neuron, 109(15), 2365-2367.

Tan, B., Chen, X., Fan, Y., Yang, Y., Yang, J., & Tan, L. (2021). STAT3 phosphorylation is required for the HepaCAM‐mediated inhibition of castration‐resistant prostate cancer cell viability and metastasis. The Prostate, 81(9), 603-611.

Fan, Y., Ou, L., Fan, J., Li, L., Wu, X., Luo, C., ... & Niu, L. (2019). HepaCAM regulates warburg effect of renal cell carcinoma via HIF-1α/NF-κB signaling pathway. Urology, 127, 61-67.

Du, Z., Li, L., Sun, W., Wang, X., Zhang, Y., Chen, Z., ... & Wu, X. (2018). HepaCAM inhibits the malignant behavior of castration-resistant prostate cancer cells by downregulating Notch signaling and PF-3084014 (a γ-secretase inhibitor) partly reverses the resistance of refractory prostate cancer to docetaxel and enzalutamide in vitro. International journal of oncology, 53(1), 99-112.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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For research use only. Not intended for any clinical use.

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