Human Recombinant HEPACAM protein, ECD, His Tag (V2LY-0526-LY4483)

Name: Human Recombinant HEPACAM protein, ECD, His Tag
SKU: V2LY-0526-LY4483
Rating: 5 (1 reviews)

See all HEPACAM antibodies

Compare Online Inquiry

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant HEPACAM protein, ECD, His Tag consist of Amino Acid: 1-240 and predicts a molecular mass of 24.5 kDa.

Molecule Mass

24.5 kDa

Protein Domain

ECD

Verified

HPLC

Sequence

Amino Acid: 1-240

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

≥95% as determined by SDS-PAGE. ≥95% as determined by SEC-HPLC.

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Hepatic And Glial Cell Adhesion Molecule

Function

Involved in regulating cell motility and cell-matrix interactions. May inhibit cell growth through suppression of cell proliferation.

Biological Process

Cell adhesion Source: UniProtKB-KW
Cell cycle Source: UniProtKB-KW
Cellular protein localization Source: Ensembl
Regulation of growth Source: UniProtKB-KW

Cellular Location

Cytoplasm; Membrane. In MCF-7 breast carcinoma and hepatic Hep 3B2.1-7 and Hep-G2 cell lines, localization of HEPACAM is cell density-dependent. In well spread cells, localized to punctate structures in the perinuclear membrane, cytoplasm, and at cell surface of protusions. In confluent cells, localized predominantly to the cytoplasmic membrane, particularly in areas of cell-cell contacts. Colocalizes with CDH1.

Involvement in disease

Leukoencephalopathy, megalencephalic, with subcortical cysts, 2A (MLC2A):
A neurodegenerative disorder characterized by infantile-onset macrocephaly and later onset of motor deterioration, with ataxia and spasticity, seizures, and cognitive decline of variable severity. The brain appears swollen on magnetic resonance imaging with white-matter abnormalities and subcortical cysts, in all stages of the disease.
Leukoencephalopathy, megalencephalic, with subcortical cysts, 2B (MLC2B):
A neurodegenerative disorder characterized by infantile-onset of macrocephaly and mildly delayed motor development associated with white-matter abnormalities on brain magnetic resonance imaging. The phenotype is milder that MLC2A, with better preserved cerebellar white matter and no subcortical cysts outside the temporal region. On follow-up, patients show normal or almost normal motor function. Some patients have normal intelligence, whereas others have a significant cognitive deficiency.

Topology

Extracellular: 34-240
Helical: 241-261
Cytoplasmic: 262-416

PTM

N-glycosylated.

Chen, E., Liu, N., Zhao, Y., Tang, M., Ou, L., Wu, X., & Luo, C. (2022). Panobinostat reverses HepaCAM gene expression and suppresses proliferation by increasing histone acetylation in prostate cancer. Gene, 808, 145977.

He, Z., Gao, Y., Li, T., Yu, C., Ou, L., & Luo, C. (2022). HepaCAM‑PIK3CA axis regulates the reprogramming of glutamine metabolism to inhibit prostate cancer cell proliferation. International Journal of Oncology, 60(4), 1-17.

De, A., Lattier, J. M., Morales, J. E., Kelly, J. R., Zheng, X., Chen, Z., ... & McCarty, J. H. (2022). HepaCAM Suppresses Glioblastoma Stem Cell Invasion in the Brain. bioRxiv, 2022-08.

Baldwin, K. T., Tan, C. X., Strader, S. T., Jiang, C., Savage, J. T., Elorza-Vidal, X., ... & Eroglu, C. (2021). HepaCAM controls astrocyte self-organization and coupling. Neuron, 109(15), 2427-2442.

Li, T., Liu, N., Gao, Y., Quan, Z., Hao, Y., Yu, C., ... & Wu, X. (2021). Long noncoding RNA HOTAIR regulates the invasion and metastasis of prostate cancer by targeting hepaCAM. British journal of cancer, 124(1), 247-258.

Sofroniew, M. V. (2021). HepaCAM shapes astrocyte territories, stabilizes gap-junction coupling, and influences neuronal excitability. Neuron, 109(15), 2365-2367.

Tan, B., Chen, X., Fan, Y., Yang, Y., Yang, J., & Tan, L. (2021). STAT3 phosphorylation is required for the HepaCAM‐mediated inhibition of castration‐resistant prostate cancer cell viability and metastasis. The Prostate, 81(9), 603-611.

Fan, Y., Ou, L., Fan, J., Li, L., Wu, X., Luo, C., ... & Niu, L. (2019). HepaCAM regulates warburg effect of renal cell carcinoma via HIF-1α/NF-κB signaling pathway. Urology, 127, 61-67.

Du, Z., Li, L., Sun, W., Wang, X., Zhang, Y., Chen, Z., ... & Wu, X. (2018). HepaCAM inhibits the malignant behavior of castration-resistant prostate cancer cells by downregulating Notch signaling and PF-3084014 (a γ-secretase inhibitor) partly reverses the resistance of refractory prostate cancer to docetaxel and enzalutamide in vitro. International journal of oncology, 53(1), 99-112.

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Human Recombinant HEPACAM protein, ECD, His Tag?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Related Products

Mouse Anti-HEPACAM Recombinant Antibody (CBFYM-0454) (CAT#: CBMAB-M0572-FY)

Mouse Anti-HEPACAM Recombinant Antibody (CBFYH-0927) (CAT#: CBMAB-H1861-FY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry