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Mouse Anti-HERC3 Recombinant Antibody (2C1) (CBMAB-A3824-LY)

The product is antibody recognizes HERC3. The antibody 2C1 immunoassay techniques such as: WB, ELISA.
See all HERC3 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
2C1
Antibody Isotype
IgG1, κ
Application
WB, ELISA

Basic Information

Immunogen
HERC3 (NP_055421, 1 a.a. ~ 109 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
hect domain and RLD 3
Introduction
HERC3 belongs to the HERC family of ubiquitin ligases, all of which contain a HECT domain and at least 1 RCC1 (MIM 179710)-like domain (RLD). The 350-amino acid HECT domain is predicted to catalyze the formation of a thioester with ubiquitin before transferring it to a substrate, and the RLD is predicted to act as a guanine nucleotide exchange factor for small G proteins (Hochrainer et al., 2005 [PubMed 15676274]).[supplied by OMIM
Entrez Gene ID
8916
UniProt ID
Q15034
Alternative Names
KIAA0032
Function
E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates.
Cellular Location
Cytoplasm; Cytoplasmic vesicle. Also found in vesicular-like structures.
PTM
Ubiquitinated; which promotes degradation by the proteasome.

Yuan, B., Liu, J., Shi, A., Cao, J., Yu, Y., Zhu, Y., ... & Feng, X. H. (2023). HERC3 promotes YAP/TAZ stability and tumorigenesis independently of its ubiquitin ligase activity. The EMBO Journal, 42(4), e111549.

Li, X., Wang, X., Chen, C., Zhang, E., Zhang, Y., Li, H., ... & Zhang, F. (2023). Accumulation of NCOA1 dependent on HERC3 deficiency transactivates matrix metallopeptidases and promotes extracellular matrix degradation in intervertebral disc degeneration. Life Sciences, 320, 121555.

Zhang, Z., Wu, Q., Fang, M., Liu, Y., Jiang, J., Feng, Q., ... & Xu, J. (2022). HERC3 directly targets RPL23A for ubiquitination degradation and further regulates Colorectal Cancer proliferation and the cell cycle. International Journal of Biological Sciences, 18(8), 3282.

Zhang, Z., He, G., Lv, Y., Liu, Y., Niu, Z., Feng, Q., ... & Xu, J. (2022). HERC3 regulates epithelial-mesenchymal transition by directly ubiquitination degradation EIF5A2 and inhibits metastasis of colorectal cancer. Cell death & disease, 13(1), 74.

Xu, S., Xiong, Y., Yao, R., Tian, R., Meng, Z., Zaky, M. Y., ... & Wu, H. (2022). A Novel ERK2 Degrader Z734 Induces Apoptosis of MCF–7 Cells via the HERC3/p53 Signaling Pathway. Molecules, 27(14), 4337.

Li, H., Li, J., Chen, L., Qi, S., Yu, S., Weng, Z., ... & Lu, Y. (2019). HERC3-mediated SMAD7 ubiquitination degradation promotes autophagy-induced EMT and chemoresistance in glioblastoma. Clinical Cancer Research, 25(12), 3602-3616.

Chen, Y., Li, Y., Peng, Y., Zheng, X., Fan, S., Yi, Y., ... & Li, C. (2018). ΔNp63α down-regulates c-Myc modulator MM1 via E3 ligase HERC3 in the regulation of cell senescence. Cell Death & Differentiation, 25(12), 2118-2129.

Zegeye, Y., Aredo, B., Yuksel, S., Turpin, E., Shrestha, S., Ludwig, S., ... & Ufret-Vincenty, R. (2023). Herc3 mutations lead to prominent retinal microglial/macrophage activation and retinal degeneration. Investigative Ophthalmology & Visual Science, 64(8), 1587-1587.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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