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Mouse Anti-HERC3 (AA 1-110) Recombinant Antibody (CBFYH-0932) (CBMAB-H1866-FY)

This product is mouse antibody that recognizes HERC3. The antibody CBFYH-0932 can be used for immunoassay techniques such as: ELISA, IHC-P, WB.
See all HERC3 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBFYH-0932
Antibody Isotype
IgG1, κ
Application
ELISA, IHC-P, WB

Basic Information

Immunogen
Recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human
Antibody Isotype
IgG1, κ
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
AA 1-110

Target

Full Name
hect domain and RLD 3
Introduction
This gene encodes a member the HERC ubiquitin ligase family. The encoded protein is located in the cytosol and binds ubiquitin via a HECT domain. Mutations in this gene have been associated with colorectal and gastric carcinomas. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Entrez Gene ID
8916
UniProt ID
Q15034
Alternative Names
HECT And RLD Domain Containing E3 Ubiquitin Protein Ligase 3; HECT Domain And RCC1-Like Domain-Containing Protein 3; HECT-Type E3 Ubiquitin Transferase HERC3; Hect Domain And RLD 3; EC 2.3.2.26; KIAA0032; EC 6.3.
Function
E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates.
Cellular Location
Cytoplasm; Cytoplasmic vesicle. Also found in vesicular-like structures.
PTM
Ubiquitinated; which promotes degradation by the proteasome.

Yuan, B., Liu, J., Shi, A., Cao, J., Yu, Y., Zhu, Y., ... & Feng, X. H. (2023). HERC3 promotes YAP/TAZ stability and tumorigenesis independently of its ubiquitin ligase activity. The EMBO Journal, 42(4), e111549.

Li, X., Wang, X., Chen, C., Zhang, E., Zhang, Y., Li, H., ... & Zhang, F. (2023). Accumulation of NCOA1 dependent on HERC3 deficiency transactivates matrix metallopeptidases and promotes extracellular matrix degradation in intervertebral disc degeneration. Life Sciences, 320, 121555.

Zhang, Z., Wu, Q., Fang, M., Liu, Y., Jiang, J., Feng, Q., ... & Xu, J. (2022). HERC3 directly targets RPL23A for ubiquitination degradation and further regulates Colorectal Cancer proliferation and the cell cycle. International Journal of Biological Sciences, 18(8), 3282.

Zhang, Z., He, G., Lv, Y., Liu, Y., Niu, Z., Feng, Q., ... & Xu, J. (2022). HERC3 regulates epithelial-mesenchymal transition by directly ubiquitination degradation EIF5A2 and inhibits metastasis of colorectal cancer. Cell death & disease, 13(1), 74.

Xu, S., Xiong, Y., Yao, R., Tian, R., Meng, Z., Zaky, M. Y., ... & Wu, H. (2022). A Novel ERK2 Degrader Z734 Induces Apoptosis of MCF–7 Cells via the HERC3/p53 Signaling Pathway. Molecules, 27(14), 4337.

Li, H., Li, J., Chen, L., Qi, S., Yu, S., Weng, Z., ... & Lu, Y. (2019). HERC3-mediated SMAD7 ubiquitination degradation promotes autophagy-induced EMT and chemoresistance in glioblastoma. Clinical Cancer Research, 25(12), 3602-3616.

Chen, Y., Li, Y., Peng, Y., Zheng, X., Fan, S., Yi, Y., ... & Li, C. (2018). ΔNp63α down-regulates c-Myc modulator MM1 via E3 ligase HERC3 in the regulation of cell senescence. Cell Death & Differentiation, 25(12), 2118-2129.

Zegeye, Y., Aredo, B., Yuksel, S., Turpin, E., Shrestha, S., Ludwig, S., ... & Ufret-Vincenty, R. (2023). Herc3 mutations lead to prominent retinal microglial/macrophage activation and retinal degeneration. Investigative Ophthalmology & Visual Science, 64(8), 1587-1587.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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