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Mouse Anti-HERC4 Recombinant Antibody (CBFYH-0934) (CBMAB-H0672-FY)

This product is mouse antibody that recognizes HERC4. The antibody CBFYH-0934 can be used for immunoassay techniques such as: ELISA, WB.
See all HERC4 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBFYH-0934
Antibody Isotype
IgG2a, κ
Application
ELISA, WB

Basic Information

Immunogen
Recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human
Antibody Isotype
IgG2a, κ
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.2
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
hect domain and RLD 4
Introduction
HERC4 belongs to the HERC family of ubiquitin ligases, all of which contain a HECT domain and at least 1 RCC1 (MIM 179710)-like domain (RLD). The 350-amino acid HECT domain is predicted to catalyze the formation of a thioester with ubiquitin before transferring it to a substrate, and the RLD is predicted to act as a guanine nucleotide exchange factor for small G proteins (Hochrainer et al., 2005 [PubMed 15676274]).
Entrez Gene ID
26091
UniProt ID
Q5GLZ8
Alternative Names
HECT And RLD Domain Containing E3 Ubiquitin Protein Ligase 4; HECT Domain And RCC1-Like Domain-Containing Protein 4; HECT-Type E3 Ubiquitin Transferase HERC4; Hect Domain And RLD 4; EC 2.3.2.26; KIAA1593; EC 6.3.
Function
Probable E3 ubiquitin-protein ligase involved in either protein trafficking or in the distribution of cellular structures. Required for spermatozoon maturation and fertility, and for the removal of the cytoplasmic droplet of the spermatozoon. E3 ubiquitin-protein ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer it to targeted substrates.
Biological Process
Cell differentiation Source: UniProtKB-KW
Negative regulation of smoothened signaling pathway Source: FlyBase
Spermatogenesis Source: UniProtKB
Cellular Location
Cytosol. Shows a punctate cytoplasmic distribution.

Mutlu, M., Schmidt, I., Morrison, A. I., Goretzki, B., Freuler, F., Begue, D., ... & Guerini, D. (2023). A novel HERC4-dependent glue degrader targeting STING. BioRxiv, 2023-02.

Zhang, Z., Li, M., Lin, P., Ren, Y., He, Y., Wang, S., ... & Mao, X. (2023). The ubiquitin ligase HERC4 suppresses MafA transcriptional activity triggered by GSK3β in myeloma by atypical K63-linked polyubiquitination. Journal of Biological Chemistry, 299(5).

Huang, F., Tang, X., Sun, T., Wang, G., Ru, Q., & Zheng, Y. (2021). SAV1, regulated by HERC4, inhibits the proliferation, migration, and invasion of hepatocellular carcinoma. Translational Cancer Research, 10(1), 349.

Sun, X., Sun, B., Cui, M., & Zhou, Z. (2019). HERC4 exerts an anti-tumor role through destabilizing the oncoprotein Smo. Biochemical and biophysical research communications, 513(4), 1013-1018.

Xu, Y., Ji, K., Wu, M., Hao, B., Yao, K. T., & Xu, Y. (2019). A miRNA-HERC4 pathway promotes breast tumorigenesis by inactivating tumor suppressor LATS1. Protein & cell, 10(8), 595-605.

Jiang, W., Yao, X., Shan, Z., Li, W., Gao, Y., & Zhang, Q. (2019). E3 ligase Herc4 regulates Hedgehog signalling through promoting Smoothened degradation. Journal of Molecular Cell Biology, 11(9), 791-803.

Yang, G., Hamadeh, I. S., Katz, J., Riva, A., Lakatos, P., Balla, B., ... & Gong, Y. (2018). SIRT1/HERC4 locus associated with bisphosphonate‐induced osteonecrosis of the jaw: an exome‐wide association analysis. Journal of Bone and Mineral Research, 33(1), 91-98.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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