Mouse Anti-HK2 Recombinant Antibody (
8E8) (V2LY-0725-LY248)

Name: Mouse Anti-HK2 Recombinant Antibody (8E8)
SKU: V2LY-0725-LY248
Rating: 5 (1 reviews)

See all HK2 antibodies

Compare Online Inquiry

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

8E8

Application

ELISA, WB, FC

Immunogen

Recombinant fragment corresponding to amino acids 270-435 of Human HK2 (N-terminus).

Host Species

Mouse

Specificity

Human, Pig, Mouse, Rat

Antibody Isotype

IgG1

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:5,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

Tris-Glycine, Glycerol

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Hexokinase 2

Entrez Gene ID

3099

UniProt ID

P52789

Function

Catalyzes the phosphorylation of hexose, such as D-glucose and D-fructose, to hexose 6-phosphate (D-glucose 6-phosphate and D-fructose 6-phosphate, respectively) (PubMed:23185017, PubMed:26985301, PubMed:29298880).

Mediates the initial step of glycolysis by catalyzing phosphorylation of D-glucose to D-glucose 6-phosphate (PubMed:29298880).

Plays a key role in maintaining the integrity of the outer mitochondrial membrane by preventing the release of apoptogenic molecules from the intermembrane space and subsequent apoptosis (PubMed:18350175).

Biological Process

Apoptotic mitochondrial changes Source: MGI
Canonical glycolysis Source: Reactome
Cellular glucose homeostasis Source: GO_Central
Cellular response to leukemia inhibitory factor Source: Ensembl
Establishment of protein localization to mitochondrion Source: ParkinsonsUK-UCL
Fructose 6-phosphate metabolic process Source: UniProtKB
Glucose 6-phosphate metabolic process Source: UniProtKB
Glycolytic process Source: GO_Central
Lactation Source: Ensembl
Maintenance of protein location in mitochondrion Source: ParkinsonsUK-UCL
Negative regulation of mitochondrial membrane permeability Source: Ensembl
Negative regulation of reactive oxygen species metabolic process Source: Ensembl
Positive regulation of angiogenesis Source: BHF-UCL
Positive regulation of autophagy of mitochondrion in response to mitochondrial depolarization Source: ParkinsonsUK-UCL
Regulation of glucose import Source: Ensembl
Response to hypoxia Source: Ensembl
Response to ischemia Source: Ensembl

Cellular Location

Mitochondrion outer membrane; Cytosol. The mitochondrial-binding peptide (MBP) region promotes association with the mitochondrial outer membrane (PubMed:29298880). The interaction with the mitochondrial outer membrane via the mitochondrial-binding peptide (MBP) region promotes higher stability of the protein (PubMed:29298880). Release from the mitochondrial outer membrane into the cytosol induces permeability transition pore (PTP) opening and apoptosis (PubMed:18350175).

Wang, S., Zhuang, Y., Xu, J., Tong, Y., Li, X., & Dong, C. (2023). Advances in the Study of Hexokinase 2 (HK2) Inhibitors. Anti-Cancer Agents in Medicinal Chemistry (Formerly Current Medicinal Chemistry-Anti-Cancer Agents), 23(7), 736-746.

Li, R., Mei, S., Ding, Q., Wang, Q., Yu, L., & Zi, F. (2022). A pan-cancer analysis of the role of hexokinase II (HK2) in human tumors. Scientific Reports, 12(1), 18807.

Ciscato, F., Ferrone, L., Masgras, I., Laquatra, C., & Rasola, A. (2021). Hexokinase 2 in cancer: a prima donna playing multiple characters. International journal of molecular sciences, 22(9), 4716.

Hinrichsen, F., Hamm, J., Westermann, M., Schröder, L., Shima, K., Mishra, N., ... & Sommer, F. (2021). Microbial regulation of hexokinase 2 links mitochondrial metabolism and cell death in colitis. Cell Metabolism, 33(12), 2355-2366.

Rabbani, N., & Thornalley, P. J. (2019). Hexokinase-2 glycolytic overload in diabetes and ischemia–reperfusion injury. Trends in Endocrinology & Metabolism, 30(7), 419-431.

Bao, F., Yang, K., Wu, C., Gao, S., Wang, P., Chen, L., & Li, H. (2018). New natural inhibitors of hexokinase 2 (HK2): Steroids from Ganoderma sinense. Fitoterapia, 125, 123-129.

Jiao, L., Zhang, H. L., Li, D. D., Yang, K. L., Tang, J., Li, X., ... & Zhu, X. F. (2018). Regulation of glycolytic metabolism by autophagy in liver cancer involves selective autophagic degradation of HK2 (hexokinase 2). Autophagy, 14(4), 671-684.

Bustamante, M. F., Oliveira, P. G., Garcia-Carbonell, R., Croft, A. P., Smith, J. M., Serrano, R. L., ... & Guma, M. (2018). Hexokinase 2 as a novel selective metabolic target for rheumatoid arthritis. Annals of the rheumatic diseases, 77(11), 1636-1643.

Yang, T., Ren, C., Qiao, P., Han, X., Wang, L., Lv, S., ... & Yu, Z. (2018). PIM2-mediated phosphorylation of hexokinase 2 is critical for tumor growth and paclitaxel resistance in breast cancer. Oncogene, 37(45), 5997-6009.

Zhang, X. Y., Zhang, M., Cong, Q., Zhang, M. X., Zhang, M. Y., Lu, Y. Y., & Xu, C. J. (2018). Hexokinase 2 confers resistance to cisplatin in ovarian cancer cells by enhancing cisplatin-induced autophagy. The international journal of biochemistry & cell biology, 95, 9-16.

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Mouse Anti-HK2 Recombinant Antibody (
8E8)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Related Products

Mouse Anti-HK2 (AA 1-917) Recombinant Antibody (CBFYH-1226) (CAT#: CBMAB-H0378-FY)

Rabbit Anti-HK2 (AA 325-444) Recombinant Antibody (2H8L6) (CAT#: CBMAB-H2196-FY)

Mouse Anti-HK2 Recombinant Antibody (A191) (CAT#: CBMAB-AP3073LY)

Mouse Anti-HK2 Recombinant Antibody (CBFYH-1236) (CAT#: CBMAB-H2208-FY)

Mouse Anti-HK2 Recombinant Antibody (CBT2955) (CAT#: V2LY-0625-LY2446)

Rabbit Anti-HK2 Recombinant Antibody (H.738.7) (CAT#: CBMAB-H2198-FY)

Mouse Anti-HK2 Recombinant Antibody (A190) (CAT#: CBMAB-AP9628LY)

Mouse Anti-HK2 Recombinant Antibody (A189) (CAT#: CBMAB-AP2996LY)

Mouse Anti-HK2 Recombinant Antibody (4H1) (CAT#: CBMAB-A3928-LY)

Mouse Anti-HK2 Recombinant Antibody (CBT760) (CAT#: V2LY-0625-LY3300)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control