Mouse Anti-HLA-DMA (AA 10-300) Recombinant Antibody (5C9NB) (CBMAB-H0523-FY)

Name: Mouse Anti-HLA-DMA (AA 10-300) Recombinant Antibody (5C9NB)
SKU: CBMAB-H0523-FY
Rating: 5 (1 reviews)

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Datasheet

SDS

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Basic Information

Host Animal

Mouse

Clone

5C9NB

Application

WB, IHC, IF, IHC-P, FC

Immunogen

Partial recombinant human HLA DMA protein

Specificity

Human

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Concentration

1 mg/mL

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

AA 10-300

More Infomation

Target

Full Name

Major Histocompatibility Complex, Class II, DM Alpha

Introduction

HLA-DMA belongs to the HLA class II alpha chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DMA) and a beta chain (DMB), both anchored in the membrane. It is located in intracellular vesicles. DM plays a central role in the peptide loading of MHC class II molecules by helping to release the CLIP molecule from the peptide binding site. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The alpha chain is approximately 33-35 kDa and its gene contains 5 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and the cytoplasmic tail.

Entrez Gene ID

3108

UniProt ID

P28067

Alternative Names

Major Histocompatibility Complex, Class II, DM Alpha; Really Interesting New Gene 6 Protein; MHC Class II Antigen DMA; RING6; DMA; HLA Class II Histocompatibility Antigen, DM Alpha Chain; Class II Histocompatibility Antigen, M Alpha Chain; D6S222E; HLADM

Function

Plays a critical role in catalyzing the release of class II-associated invariant chain peptide (CLIP) from newly synthesized MHC class II molecules and freeing the peptide binding site for acquisition of antigenic peptides. In B-cells, the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO.

Biological Process

Adaptive immune response Source: UniProtKB-KW
Antigen processing and presentation of exogenous peptide antigen via MHC class II Source: GO_Central
Peptide antigen assembly with MHC class II protein complex Source: UniProtKB
Positive regulation of T cell activation Source: GO_Central

Cellular Location

Lysosome membrane; Late endosome membrane. Localizes to late endocytic compartment. Associates with lysosome membranes.

Topology

Lumenal: 27-233
Helical: 234-254
Cytoplasmic: 255-261

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Antibody Pairs

HLA-DMA Matched Antibody Pair (545) (CAT#: APMAB-545LY)

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Mouse Anti-HLA-DMA Monoclonal Antibody (CBFYH-3099) (CAT#: CBMAB-H0233-FY)

HLA-DMA Matched Antibody Pair (545) (CAT#: APMAB-545LY)

Mouse Anti-HLA-DMA (AA 1-262) Monoclonal Antibody (CBFYH-3100) (CAT#: CBMAB-H2287-FY)

Mouse Anti-HLA-DMA Monoclonal Antibody (CBFYH-3101) (CAT#: CBMAB-H2288-FY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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