Mouse Anti-HLA-DOB Recombinant Antibody (DOB 10E6/5) (CBMAB-H0105-FY)

Name: Mouse Anti-HLA-DOB Recombinant Antibody (DOB 10E6/5)
SKU: CBMAB-H0105-FY
Rating: 5 (1 reviews)

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Datasheet

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Basic Information

Host Animal

Mouse

Clone

DOB 10E6/5

Application

WB, ELISA

Immunogen

C-terminal peptide MHC class II

Specificity

Human

Antibody Isotype

IgG1

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Major Histocompatibility Complex, Class II, DO Beta

Introduction

HLA-DOB belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DOA) and a beta chain (DOB), both anchored in the membrane. It is located in intracellular vesicles. DO suppresses peptide loading of MHC class II molecules by inhibiting HLA-DM. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail.

Entrez Gene ID

3112

UniProt ID

P13765

Alternative Names

Major Histocompatibility Complex, Class II, DO Beta; MHC Class II Antigen DOB; HLA Class II Histocompatibility Antigen, DO Beta Chain; HLA_DOB; DOB

Function

Important modulator in the HLA class II restricted antigen presentation pathway by interaction with the HLA-DM molecule in B-cells. Modifies peptide exchange activity of HLA-DM.

Biological Process

Adaptive immune response Source: UniProtKB-KW
Antigen processing and presentation of exogenous peptide antigen via MHC class II Source: GO_Central
Negative regulation of antigen processing and presentation of peptide antigen via MHC class II Source: UniProtKB
Peptide antigen assembly with MHC class II protein complex Source: GO_Central
Positive regulation of T cell activation Source: GO_Central

Cellular Location

Lysosome membrane; Endosome membrane. Complexes with HLA-DM molecule during intracellular transport and in endosomal/lysosomal compartments. Heterotetramerization is necessary to exit the ER.

Topology

Extracellular: 27-224
Helical: 225-245
Cytoplasmic: 246-273

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Related Products

Rabbit Anti-HLA-DOB Recombinant Antibody (EG1518) (CAT#: CBMAB-EN1816-LY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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