Rabbit Anti-HMGCL Recombinant Antibody (
22H11) (V2LY-0725-LY990)

Name: Rabbit Anti-HMGCL Recombinant Antibody (22H11)
SKU: V2LY-0725-LY990
Rating: 5 (1 reviews)

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Tested Data

Rabbit Anti-HMGCL Recombinant Antibody (22H11) (V2LY-0725-LY990) in WB

Western Blot
Positive WB detected in: Hela whole cell lysate, PC-3 whole cell lysate, HEK293 whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate
All lanes: HMGCL antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 35, 27, 21 kDa
Observed band size: 35 kDa

Rabbit Anti-HMGCL Recombinant Antibody (22H11) (V2LY-0725-LY990) in IHC

IHC image of V2LY-0725-LY990 diluted at 1:100 and staining in paraffin-embedded human prostate tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

Rabbit Anti-HMGCL Recombinant Antibody (22H11) (V2LY-0725-LY990) in FC

Overlay Peak curve showing Hela cells stained with V2LY-0725-LY990 (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4°C.Control antibody (green line) was rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of>10,000 events was performed.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

22H11

Application

ELISA, WB, IHC, FC

Immunogen

A synthesized peptide derived from human HMGCL.

Host Species

Rabbit

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:2,000
IHC	1:50-1:200
FC	1:50-1:200

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, glycerol

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

3-Hydroxymethyl-3-Methylglutaryl-CoA Lyase

Entrez Gene ID

3155

UniProt ID

P35914

Function

Mitochondrial 3-hydroxymethyl-3-methylglutaryl-CoA lyase that catalyzes a cation-dependent cleavage of (S)-3-hydroxy-3-methylglutaryl-CoA into acetyl-CoA and acetoacetate, a key step in ketogenesis. Terminal step in leucine catabolism. Ketone bodies (beta-hydroxybutyrate, acetoacetate and acetone) are essential as an alternative source of energy to glucose, as lipid precursors and as regulators of metabolism.

Biological Process

Ketone body biosynthetic process Source: UniProtKB
Leucine catabolic process Source: GO_Central
Lipid metabolic process Source: BHF-UCL
Mitochondrion organization Source: Ensembl

Cellular Location

Mitochondrion matrix; Peroxisome. Unprocessed form is peroxisomal.

Involvement in disease

3-hydroxy-3-methylglutaryl-CoA lyase deficiency (HMGCLD):
An autosomal recessive disease affecting ketogenesis and L-leucine catabolism. The disease usually appears in the first year of life after a fasting period and its clinical acute symptoms include vomiting, seizures, metabolic acidosis, hypoketotic hypoglycemia and lethargy. These symptoms sometimes progress to coma, with fatal outcome in some cases.

Zhong, C., Xiong, G., Yang, H., Du, X., Du, J., Yao, F., ... & Deng, Y. (2023). Phosphorylation by IKKβ promotes the degradation of HMGCL via NEDD4 in lung cancer. International Journal of Biological Sciences, 19(4), 1110.

Cui, X., Yun, X., Sun, M., Li, R., Lyu, X., Lao, Y., ... & Yu, W. (2023). HMGCL-induced β-hydroxybutyrate production attenuates hepatocellular carcinoma via DPP4-mediated ferroptosis susceptibility. Hepatology International, 17(2), 377-392.

Li, Y., Fan, Z., Yin, D., Jiang, R., Deng, J., & Song, X. (2022). HMGCL: Heterogeneous multigraph contrastive learning for LBSN friend recommendation. World Wide Web, 1-24.

Kil, E. J. B. (2018). CRISPR-Cas9 mediated HMGCL KO in 3xTg AD mice reduces the cognitive deficit improvement seen in an intermittent metabolic switching regimen.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Antibody Pairs

HMGCL Matched Antibody Pair (560) (CAT#: APMAB-560LY)

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HMGCL Matched Antibody Pair (560) (CAT#: APMAB-560LY)

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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