Cattle HMGCL ELISA Kit (V2LY-0626-LY2543)

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Tested Data
Datasheet
SDS
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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity
0.0073 ng/mL
Detection Range
0.015-3 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Cattle
Assay Type
Sandwich
Reactivity
Cattle
Assay Time
1.5 h
Molecule Mass
34.2 kDa
Components
  • Pre-coated ELISA plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
3-Hydroxymethyl-3-Methylglutaryl-CoA Lyase
Function
Mitochondrial 3-hydroxymethyl-3-methylglutaryl-CoA lyase that catalyzes a cation-dependent cleavage of (S)-3-hydroxy-3-methylglutaryl-CoA into acetyl-CoA and acetoacetate, a key step in ketogenesis. Terminal step in leucine catabolism. Ketone bodies (beta-hydroxybutyrate, acetoacetate and acetone) are essential as an alternative source of energy to glucose, as lipid precursors and as regulators of metabolism.
Biological Process
Ketone body biosynthetic process Source: UniProtKB
Leucine catabolic process Source: GO_Central
Lipid metabolic process Source: BHF-UCL
Mitochondrion organization Source: Ensembl
Cellular Location
Mitochondrion matrix; Peroxisome. Unprocessed form is peroxisomal.
Involvement in disease
3-hydroxy-3-methylglutaryl-CoA lyase deficiency (HMGCLD):
An autosomal recessive disease affecting ketogenesis and L-leucine catabolism. The disease usually appears in the first year of life after a fasting period and its clinical acute symptoms include vomiting, seizures, metabolic acidosis, hypoketotic hypoglycemia and lethargy. These symptoms sometimes progress to coma, with fatal outcome in some cases.

Zhong, C., Xiong, G., Yang, H., Du, X., Du, J., Yao, F., ... & Deng, Y. (2023). Phosphorylation by IKKβ promotes the degradation of HMGCL via NEDD4 in lung cancer. International Journal of Biological Sciences, 19(4), 1110.

Cui, X., Yun, X., Sun, M., Li, R., Lyu, X., Lao, Y., ... & Yu, W. (2023). HMGCL-induced β-hydroxybutyrate production attenuates hepatocellular carcinoma via DPP4-mediated ferroptosis susceptibility. Hepatology International, 17(2), 377-392.

Li, Y., Fan, Z., Yin, D., Jiang, R., Deng, J., & Song, X. (2022). HMGCL: Heterogeneous multigraph contrastive learning for LBSN friend recommendation. World Wide Web, 1-24.

Kil, E. J. B. (2018). CRISPR-Cas9 mediated HMGCL KO in 3xTg AD mice reduces the cognitive deficit improvement seen in an intermittent metabolic switching regimen.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

HMGCL Matched Antibody Pair (560) (CAT#: APMAB-560LY)

Antibody Pairs

HMGCL Matched Antibody Pair (560) (CAT#: APMAB-560LY)

Related Products

Rabbit Anti-HMGCL Recombinant Antibody (
22H11) (CAT#: V2LY-0725-LY990)

HMGCL Matched Antibody Pair (560) (CAT#: APMAB-560LY)

Mouse Anti-HMGCL Recombinant Antibody (4F4-D1) (CAT#: CBMAB-A3974-LY)

Mouse Anti-HMGCL Recombinant Antibody (CBFYH-1545) (CAT#: CBMAB-H2520-FY)

Mouse Anti-HMGCL Recombinant Antibody (9F5AB9) (CAT#: CBMAB-H2517-FY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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