Mouse Anti-HMGCS2 (AA 424-508) Recombinant Antibody (CBFYH-1550) (CBMAB-H2525-FY)

Name: Mouse Anti-HMGCS2 (AA 424-508) Recombinant Antibody (CBFYH-1550)
SKU: CBMAB-H2525-FY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBFYH-1550

Application

ELISA, WB

Immunogen

HMGCS2 AA 424-508 partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

AA 424-508

More Infomation

Target

Full Name

3-Hydroxy-3-Methylglutaryl-CoA Synthase 2

Introduction

The protein encoded by this gene belongs to the HMG-CoA synthase family. It is a mitochondrial enzyme that catalyzes the first reaction of ketogenesis, a metabolic pathway that provides lipid-derived energy for various organs during times of carbohydrate deprivation, such as fasting. Mutations in this gene are associated with HMG-CoA synthase deficiency. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Entrez Gene ID

3158

UniProt ID

P54868

Alternative Names

3-Hydroxy-3-Methylglutaryl-CoA Synthase 2; EC 2.3.3.10; 3-Hydroxy-3-Methylglutaryl-Coenzyme A Synthase 2 (Mitochondrial); 3-Hydroxy-3-Methylglutaryl-CoA Synthase 2 (Mitochondrial); 3-Hydroxy-3-Methylglutaryl Coenzyme A Synthase; HMG-CoA Synthase

Function

Catalyzes the first irreversible step in ketogenesis, condensing acetyl-CoA to acetoacetyl-CoA to form HMG-CoA, which is converted by HMG-CoA reductase (HMGCR) into mevalonate.

Biological Process

Acetyl-CoA metabolic process Source: UniProtKB
Adipose tissue development Source: Ensembl
Brain development Source: Ensembl
Cellular response to amino acid stimulus Source: Ensembl
Cellular response to fatty acid Source: Ensembl
Cellular response to glucocorticoid stimulus Source: Ensembl
Cellular response to insulin stimulus Source: Ensembl
Cellular response to lipopolysaccharide Source: Ensembl
Cholesterol biosynthetic process Source: UniProtKB-KW
Farnesyl diphosphate biosynthetic process, mevalonate pathway Source: GO_Central
Ketone body biosynthetic process Source: UniProtKB
Kidney development Source: Ensembl
Liver development Source: Ensembl
Lung development Source: Ensembl
Midgut development Source: Ensembl
Multicellular organismal response to stress Source: Ensembl
Response to cAMP Source: Ensembl
Response to ethanol Source: Ensembl
Response to glucagon Source: Ensembl
Response to growth hormone Source: Ensembl
Response to linoleic acid Source: Ensembl
Response to metal ion Source: Ensembl
Response to monosaccharide Source: Ensembl
Response to nutrient Source: Ensembl
Response to prostaglandin F Source: Ensembl
Response to starvation Source: Ensembl
Response to temperature stimulus Source: Ensembl
Response to testosterone Source: Ensembl
Response to triglyceride Source: Ensembl
Response to xenobiotic stimulus Source: Ensembl

Cellular Location

Mitochondrion

Involvement in disease

3-hydroxy-3-methylglutaryl-CoA synthase-2 deficiency (HMGCS2D):
A metabolic disorder characterized by severe hypoketotic hypoglycemia, encephalopathy, and hepatomegaly.

PTM

Succinylated. Desuccinylated by SIRT5. Succinylation, at least at Lys-83 and Lys-310, inhibits the enzymatic activity.

Bai, M., Wu, M., Jiang, M., He, J., Deng, X., Xu, S., ... & Zhang, A. (2023). LONP1 targets HMGCS2 to protect mitochondrial function and attenuate chronic kidney disease. EMBO Molecular Medicine, 15(2), e16581.

Asif, S., Kim, R. Y., Fatica, T., Sim, J., Zhao, X., Oh, Y., ... & Kim, K. H. (2022). Hmgcs2-mediated ketogenesis modulates high-fat diet-induced hepatosteatosis. Molecular metabolism, 61, 101494.

Venable, A. H., Lee, L. E., Feola, K., Santoyo, J., Broomfield, T., & Huen, S. C. (2022). Fasting-induced HMGCS2 expression in the kidney does not contribute to circulating ketones. American Journal of Physiology-Renal Physiology, 322(4), F460-F467.

Li, J., Li, M. H., Wang, T. T., Liu, X. N., Zhu, X. T., Dai, Y. Z., ... & Yuan, J. H. (2021). SLC38A4 functions as a tumour suppressor in hepatocellular carcinoma through modulating Wnt/β-catenin/MYC/HMGCS2 axis. British Journal of Cancer, 125(6), 865-876.

Li, S., Qian, X., Gong, J., Chen, J. I. E., Tu, W., Chen, X., ... & Jiang, S. (2021). Exercise training reverses lipotoxicity-induced cardiomyopathy by inhibiting HMGCS2. Medicine and science in sports and exercise, 53(1), 47-57.

Zou, K., Hu, Y., Li, M., Wang, H., Zhang, Y., Huang, L., ... & Wang, Y. (2019). Potential role of HMGCS2 in tumor angiogenesis in colorectal cancer and its potential use as a diagnostic marker. Canadian Journal of Gastroenterology and Hepatology, 2019.

Wan, S., Xi, M., Zhao, H. B., Hua, W., Liu, Y. L., Zhou, Y. L., ... & Zhong, W. D. (2019). HMGCS2 functions as a tumor suppressor and has a prognostic impact in prostate cancer. Pathology-Research and Practice, 215(8), 152464.

Wang, Y. H., Liu, C. L., Chiu, W. C., Twu, Y. C., & Liao, Y. J. (2019). HMGCS2 mediates ketone production and regulates the proliferation and metastasis of hepatocellular carcinoma. Cancers, 11(12), 1876.

Kim, J. T., Li, C., Weiss, H. L., Zhou, Y., Liu, C., Wang, Q., & Evers, B. M. (2019). Regulation of ketogenic enzyme HMGCS2 by Wnt/β-catenin/PPARγ pathway in intestinal cells. Cells, 8(9), 1106.

Jebb, D., & Hiller, M. (2018). Recurrent loss of HMGCS2 shows that ketogenesis is not essential for the evolution of large mammalian brains. Elife, 7, e38906.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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For research use only. Not intended for any clinical use.

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