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Rabbit Anti-HMGCS2 Recombinant Antibody (D3U1A) (CBMAB-CP1036-LY)

The product is antibody recognizes HMGCS2. The antibody D3U1A immunoassay techniques such as: WB,IP.
See all HMGCS2 antibodies

Summary

Host Animal
Rabbit
Specificity
Human, Mouse, Rat
Clone
D3U1A
Antibody Isotype
IgG
Application
WB, IP

Basic Information

Immunogen
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro478 of human HMGCS2 protein.
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
100 µg/ml BSA, 50% glycerol
Preservative
0.02% sodium azide
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
3-Hydroxy-3-Methylglutaryl-CoA Synthase 2
Introduction
The protein encoded by this gene belongs to the HMG-CoA synthase family. It is a mitochondrial enzyme that catalyzes the first reaction of ketogenesis, a metabolic pathway that provides lipid-derived energy for various organs during times of carbohydrate deprivation, such as fasting. Mutations in this gene are associated with HMG-CoA synthase deficiency. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Oct 2009]
Entrez Gene ID
Human3158
Mouse15360
Rat24450
UniProt ID
HumanP54868
MouseP54869
RatP22791
Alternative Names
3-Hydroxy-3-Methylglutaryl-CoA Synthase 2; EC 2.3.3.10; 3-Hydroxy-3-Methylglutaryl-Coenzyme A Synthase 2 (Mitochondrial); 3-Hydroxy-3-Methylglutaryl-CoA Synthase 2 (Mitochondrial); 3-Hydroxy-3-Methylglutaryl Coenzyme A Synthase; HMG-CoA Synthase;
Function
Catalyzes the first irreversible step in ketogenesis, condensing acetyl-CoA to acetoacetyl-CoA to form HMG-CoA, which is converted by HMG-CoA reductase (HMGCR) into mevalonate.
Biological Process
Acetyl-CoA metabolic process Source: UniProtKB
Adipose tissue development Source: Ensembl
Brain development Source: Ensembl
Cellular response to amino acid stimulus Source: Ensembl
Cellular response to fatty acid Source: Ensembl
Cellular response to glucocorticoid stimulus Source: Ensembl
Cellular response to insulin stimulus Source: Ensembl
Cellular response to lipopolysaccharide Source: Ensembl
Cholesterol biosynthetic process Source: UniProtKB-KW
Farnesyl diphosphate biosynthetic process, mevalonate pathway Source: GO_Central
Ketone body biosynthetic process Source: UniProtKB
Kidney development Source: Ensembl
Liver development Source: Ensembl
Lung development Source: Ensembl
Midgut development Source: Ensembl
Multicellular organismal response to stress Source: Ensembl
Response to cAMP Source: Ensembl
Response to ethanol Source: Ensembl
Response to glucagon Source: Ensembl
Response to growth hormone Source: Ensembl
Response to linoleic acid Source: Ensembl
Response to metal ion Source: Ensembl
Response to monosaccharide Source: Ensembl
Response to nutrient Source: Ensembl
Response to prostaglandin F Source: Ensembl
Response to starvation Source: Ensembl
Response to temperature stimulus Source: Ensembl
Response to testosterone Source: Ensembl
Response to triglyceride Source: Ensembl
Response to xenobiotic stimulus Source: Ensembl
Cellular Location
Mitochondrion
Involvement in disease
3-hydroxy-3-methylglutaryl-CoA synthase-2 deficiency (HMGCS2D):
A metabolic disorder characterized by severe hypoketotic hypoglycemia, encephalopathy, and hepatomegaly.
PTM
Succinylated. Desuccinylated by SIRT5. Succinylation, at least at Lys-83 and Lys-310, inhibits the enzymatic activity.

Bai, M., Wu, M., Jiang, M., He, J., Deng, X., Xu, S., ... & Zhang, A. (2023). LONP1 targets HMGCS2 to protect mitochondrial function and attenuate chronic kidney disease. EMBO Molecular Medicine, 15(2), e16581.

Asif, S., Kim, R. Y., Fatica, T., Sim, J., Zhao, X., Oh, Y., ... & Kim, K. H. (2022). Hmgcs2-mediated ketogenesis modulates high-fat diet-induced hepatosteatosis. Molecular metabolism, 61, 101494.

Venable, A. H., Lee, L. E., Feola, K., Santoyo, J., Broomfield, T., & Huen, S. C. (2022). Fasting-induced HMGCS2 expression in the kidney does not contribute to circulating ketones. American Journal of Physiology-Renal Physiology, 322(4), F460-F467.

Li, J., Li, M. H., Wang, T. T., Liu, X. N., Zhu, X. T., Dai, Y. Z., ... & Yuan, J. H. (2021). SLC38A4 functions as a tumour suppressor in hepatocellular carcinoma through modulating Wnt/β-catenin/MYC/HMGCS2 axis. British Journal of Cancer, 125(6), 865-876.

Li, S., Qian, X., Gong, J., Chen, J. I. E., Tu, W., Chen, X., ... & Jiang, S. (2021). Exercise training reverses lipotoxicity-induced cardiomyopathy by inhibiting HMGCS2. Medicine and science in sports and exercise, 53(1), 47-57.

Zou, K., Hu, Y., Li, M., Wang, H., Zhang, Y., Huang, L., ... & Wang, Y. (2019). Potential role of HMGCS2 in tumor angiogenesis in colorectal cancer and its potential use as a diagnostic marker. Canadian Journal of Gastroenterology and Hepatology, 2019.

Wan, S., Xi, M., Zhao, H. B., Hua, W., Liu, Y. L., Zhou, Y. L., ... & Zhong, W. D. (2019). HMGCS2 functions as a tumor suppressor and has a prognostic impact in prostate cancer. Pathology-Research and Practice, 215(8), 152464.

Wang, Y. H., Liu, C. L., Chiu, W. C., Twu, Y. C., & Liao, Y. J. (2019). HMGCS2 mediates ketone production and regulates the proliferation and metastasis of hepatocellular carcinoma. Cancers, 11(12), 1876.

Kim, J. T., Li, C., Weiss, H. L., Zhou, Y., Liu, C., Wang, Q., & Evers, B. M. (2019). Regulation of ketogenic enzyme HMGCS2 by Wnt/β-catenin/PPARγ pathway in intestinal cells. Cells, 8(9), 1106.

Jebb, D., & Hiller, M. (2018). Recurrent loss of HMGCS2 shows that ketogenesis is not essential for the evolution of large mammalian brains. Elife, 7, e38906.

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For research use only. Not intended for any clinical use.

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