Rabbit Anti-HNRNPC Recombinant Antibody (
9G1) (V2LY-0725-LY776)

Name: Rabbit Anti-HNRNPC Recombinant Antibody (9G1)
SKU: V2LY-0725-LY776
Rating: 5 (1 reviews)

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Tested Data

Rabbit Anti-HNRNPC Recombinant Antibody (9G1) (V2LY-0725-LY776) in WB

Western Blot Positive WB detected in: Hela whole cell lysate, 293 whole cell lysate, JK whole cell lysate, Raji whole cell lysate, MCF7 whole cell lysate All lanes: HNRNPC antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 34, 33, 36, 28 kDa Observed band size: 42 kDa

Rabbit Anti-HNRNPC Recombinant Antibody (9G1) (V2LY-0725-LY776) in IHC

IHC image of V2LY-0725-LY776 diluted at 1:300 and staining in paraffin-embedded human braintissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody

Rabbit Anti-HNRNPC Recombinant Antibody (9G1) (V2LY-0725-LY776) in IHC

IHC image of V2LY-0725-LY776 diluted at 1:300 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody

Rabbit Anti-HNRNPC Recombinant Antibody (9G1) (V2LY-0725-LY776) in IHC

IHC image of V2LY-0725-LY776 diluted at 1:300 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody

Rabbit Anti-HNRNPC Recombinant Antibody (9G1) (V2LY-0725-LY776) in IF

Immunofluorescence staining of Hela cell with V2LY-0725-LY776 at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Rabbit Anti-HNRNPC Recombinant Antibody (9G1) (V2LY-0725-LY776) in IF

Immunofluorescence staining of Hela cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Rabbit Anti-HNRNPC Recombinant Antibody (9G1) (V2LY-0725-LY776) in IF

Immunofluorescence staining of HepG2 cell with V2LY-0725-LY776 at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Rabbit Anti-HNRNPC Recombinant Antibody (9G1) (V2LY-0725-LY776) in IF

Immunofluorescence staining of HepG2 cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Rabbit Anti-HNRNPC Recombinant Antibody (9G1) (V2LY-0725-LY776) in FC

Overlay Peak curve showing MCF7 cells stained with V2LY-0725-LY776 (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4°C.Control antibody (green line) was Rabit IgG (1ug/1*106cells) used under the same conditions. Acquisition of>10,000 events was performed.

Rabbit Anti-HNRNPC Recombinant Antibody (9G1) (V2LY-0725-LY776) in IP

Immunoprecipitating HNRNPC in Hela whole cell lysate Lane 1: Rabbit control IgG instead of V2LY-0725-LY776 in Hela whole cell lysate. Lane 2:V2LY-0725-LY776(3µg)+ Hela whole cell lysate(500µg) Lane 3: Hela whole cell lysate(20µg) For western blotting, Goat polyclonal to rabbit IgG antibody was used as the secondary antibody (1/50000)

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

9G1

Application

ELISA, WB, IHC, IF, FC, IP

Immunogen

A synthesized peptide derived from human HNRNPC.

Host Species

Rabbit

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:5,000
IHC	1:200-1:500
IF	1:20-1:200
IP	1:200-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, glycerol

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

heterogeneous nuclear ribonucleoprotein C (C1/C2)

Entrez Gene ID

3183

UniProt ID

P07910

Function

Binds pre-mRNA and nucleates the assembly of 40S hnRNP particles (PubMed:8264621).

Interacts with poly-U tracts in the 3'-UTR or 5'-UTR of mRNA and modulates the stability and the level of translation of bound mRNA molecules (PubMed:12509468, PubMed:16010978, PubMed:7567451, PubMed:8264621).

Single HNRNPC tetramers bind 230-240 nucleotides. Trimers of HNRNPC tetramers bind 700 nucleotides (PubMed:8264621).

May play a role in the early steps of spliceosome assembly and pre-mRNA splicing. N6-methyladenosine (m6A) has been shown to alter the local structure in mRNAs and long non-coding RNAs (lncRNAs) via a mechanism named 'm6A-switch', facilitating binding of HNRNPC, leading to regulation of mRNA splicing (PubMed:25719671).

Biological Process

3'-UTR-mediated mRNA stabilization Source: UniProtKB
Chromatin remodeling Source: GO_Central
mRNA splicing, via spliceosome Source: UniProtKB
Negative regulation of telomere maintenance via telomerase Source: BHF-UCL
Osteoblast differentiation Source: UniProtKB
RNA splicing Source: ProtInc

Cellular Location

Nucleus. Component of ribonucleosomes.

PTM

Phosphorylated on Ser-260 and Ser-299 in resting cells. Phosphorylated on Ser-253 and on 1 serine residue in the poly-Ser stretch at position 238 in response to hydrogen peroxide.
Sumoylated. Sumoylation reduces affinity for mRNA.

Mo, L., Meng, L., Huang, Z., Yi, L., Yang, N., & Li, G. (2022). An analysis of the role of HnRNP C dysregulation in cancers. Biomarker Research, 10(1), 19.

Yang, L., WenTao, T., ZhiYuan, Z., Qi, L., YuXiang, L., Peng, Z., ... & JianMin, X. (2022). Cullin-9/p53 mediates HNRNPC degradation to inhibit erastin-induced ferroptosis and is blocked by MDM2 inhibition in colorectal cancer. Oncogene, 41(23), 3210-3221.

Zhang, X., Chu, H., Chik, K. K. H., Wen, L., Shuai, H., Yang, D., ... & Chan, J. F. W. (2022). hnRNP C modulates MERS-CoV and SARS-CoV-2 replication by governing the expression of a subset of circRNAs and cognitive mRNAs. Emerging Microbes & Infections, 11(1), 519-531.

Zhu, W., Wang, J., Liu, X., Xu, Y., Zhai, R., Zhang, J., ... & Liu, L. (2022). lncRNA CYTOR promotes aberrant glycolysis and mitochondrial respiration via HNRNPC-mediated ZEB1 stabilization in oral squamous cell carcinoma. Cell Death & Disease, 13(8), 703.

Lv, W., Tan, Y., Xiong, M., Zhao, C., Wang, Y., Wu, M., ... & Zhang, Q. (2021). Analysis and validation of m6A regulatory network: a novel circBACH2/has-miR-944/HNRNPC axis in breast cancer progression. Journal of Translational Medicine, 19(1), 527.

Huang, X. T., Li, J. H., Zhu, X. X., Huang, C. S., Gao, Z. X., Xu, Q. C., ... & Yin, X. Y. (2021). HNRNPC impedes m6A-dependent anti-metastatic alternative splicing events in pancreatic ductal adenocarcinoma. Cancer letters, 518, 196-206.

Mallory, M. J., McClory, S. P., Chatrikhi, R., Gazzara, M. R., Ontiveros, R. J., & Lynch, K. W. (2020). Reciprocal regulation of hnRNP C and CELF2 through translation and transcription tunes splicing activity in T cells. Nucleic acids research, 48(10), 5710-5719.

Wang, L. C., Chen, S. H., Shen, X. L., Li, D. C., Liu, H. Y., Ji, Y. L., ... & Lv, Q. L. (2020). M6A RNA methylation regulator HNRNPC contributes to tumorigenesis and predicts prognosis in glioblastoma multiforme. Frontiers in oncology, 10, 536875.

Huang, G. Z., Wu, Q. Q., Zheng, Z. N., Shao, T. R., Chen, Y. C., Zeng, W. S., & Lv, X. Z. (2020). M6A-related bioinformatics analysis reveals that HNRNPC facilitates progression of OSCC via EMT. Aging (Albany NY), 12(12), 11667.

Wu, Y., Zhao, W., Liu, Y., Tan, X., Li, X., Zou, Q., ... & Yang, X. (2018). Function of HNRNPC in breast cancer cells by controlling the dsRNA‐induced interferon response. The EMBO journal, 37(23), e99017.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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For research use only. Not intended for any clinical use.

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