Sign in or Register   Sign in or Register
  |  

Mouse Anti-HSD17B8 (AA 57-261) Recombinant Antibody (CBFYH-2017) (CBMAB-H3028-FY)

This product is mouse antibody that recognizes HSD17B8. The antibody CBFYH-2017 can be used for immunoassay techniques such as: WB, IHC.
See all HSD17B8 antibodies

Summary

Host Animal
Mouse
Specificity
Human, Dog
Clone
CBFYH-2017
Antibody Isotype
IgG1
Application
WB, IHC

Basic Information

Immunogen
Human recombinant protein fragment corresponding to amino acids 57-261 of human HSD17B8 produced in E.coli
Specificity
Human, Dog
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, PH 7.3, 1% BSA, 50% glycerol
Preservative
0.02% Sodium azide
Concentration
1 mg/mL
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
AA 57-261

Target

Full Name
hydroxysteroid (17-beta) dehydrogenase 8
Introduction
In mice, the Ke6 protein is a 17-beta-hydroxysteroid dehydrogenase that can regulate the concentration of biologically active estrogens and androgens. It is preferentially an oxidative enzyme and inactivates estradiol, testosterone, and dihydrotestosterone. However, the enzyme has some reductive activity and can synthesize estradiol from estrone. The protein encoded by this gene is similar to Ke6 and is a member of the short-chain dehydrogenase superfamily. An alternatively spliced transcript of this gene has been detected, but the full-length nature of this variant has not been determined.
Entrez Gene ID
Human7923
Dog607895
UniProt ID
HumanQ92506
DogQ5TJF5
Alternative Names
Hydroxysteroid 17-Beta Dehydrogenase 8; 3-Ketoacyl-[Acyl-Carrier-Protein] Reductase Alpha Subunit; Short Chain Dehydrogenase/Reductase Family 30C Member 1; 3-Oxoacyl-[Acyl-Carrier-Protein] Reductase; 17-Beta-Hydroxysteroid Dehydrogenase 8; Really Interesting New Gene 2 Protein; Testosterone 17-Beta-Dehydrogenase 8; KAR Alpha Subunit; 17-Beta-HSD 8; Protein Ke6; SDR30C1; FABGL; RING2; Ke-6; HKE6
Function
Required for the solubility and assembly of the heterotetramer 3-ketoacyl-[acyl carrier protein] (ACP) reductase functional complex (KAR or KAR1) that forms part of the mitochondrial fatty acid synthase (mtFAS). Alpha-subunit of the KAR complex that acts as a scaffold protein required for the stability of carbonyl reductase type-4 (CBR4, beta-subunit of the KAR complex) and for its 3-ketoacyl-ACP reductase activity, thereby participating in mitochondrial fatty acid biosynthesis. Catalyzes the NAD-dependent conversion of (3R)-3-hydroxyacyl-CoA into 3-ketoacyl-CoA (3-oxoacyl-CoA) with no chain length preference; this enzymatic activity is not needed for the KAR function (PubMed:19571038, PubMed:25203508, PubMed:30508570).

Prefers (3R)-3-hydroxyacyl-CoA over (3S)-3-hydroxyacyl-CoA and displays enzymatic activity only in the presence of NAD+ (PubMed:19571038).

Cooperates with enoyl-CoA hydratase 1 in mitochondria, together they constitute an alternative route to the auxiliary enzyme pathways for the breakdown of Z-PUFA (cis polyunsaturated fatty acid) enoyl-esters (Probable) (PubMed:30508570).

NAD-dependent 17-beta-hydroxysteroid dehydrogenase with highest activity towards estradiol (17beta-estradiol or E2). Has very low activity towards testosterone and dihydrotestosterone (17beta-hydroxy-5alpha-androstan-3-one). Primarily an oxidative enzyme, it can switch to a reductive mode determined in the appropriate physiologic milieu and catalyze the reduction of estrone (E1) to form biologically active 17beta-estradiol (PubMed:17978863).
Biological Process
Androgen metabolic process Source: Ensembl
Estrogen biosynthetic process Source: UniProtKB
Fatty acid biosynthetic process Source: UniProtKB
Protein heterotetramerization Source: UniProtKB
Cellular Location
Mitochondrion matrix
Ask a question We look forward to hearing from you.
0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Loading...
Have you used Mouse Anti-HSD17B8 (AA 57-261) Recombinant Antibody (CBFYH-2017)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon $30 eGift Card
Submit a review
Loading...
For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Documents

Contact us

  • Tel: (USA)
  • (UK)
  • Fax:
  • Email:

Submit A Review

Go to
Compare