Mouse Anti-HSPA1L Recombinant Antibody (CBT52) (V2LY-0625-LY3837)

Name: Mouse Anti-HSPA1L Recombinant Antibody (CBT52)
SKU: V2LY-0625-LY3837
Rating: 5 (1 reviews)

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Tested Data

Mouse Anti-HSPA1L Recombinant Antibody (CBT52) (V2LY-0625-LY3837) in IF

Mouse Anti-HSPA1L Recombinant Antibody (CBT52) (V2LY-0625-LY3837) in IHC

Mouse Anti-HSPA1L Recombinant Antibody (CBT52) (V2LY-0625-LY3837) in WB

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBT52

Application

WB, IHC-P, IF, ICC

Immunogen

Synthetic Peptide of HSP70

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG

Clonality

Monoclonal Antibody

Application Notes

Application	Note
IF	1:100-1:200
IHC	1:50-1:300
WB	1:1,000-1:2,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, Glycerol, BSA

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

heat shock protein family A (Hsp70) member 1 like

Entrez Gene ID

3305

UniProt ID

P34931

Function

Molecular chaperone implicated in a wide variety of cellular processes, including protection of the proteome from stress, folding and transport of newly synthesized polypeptides, activation of proteolysis of misfolded proteins and the formation and dissociation of protein complexes. Plays a pivotal role in the protein quality control system, ensuring the correct folding of proteins, the re-folding of misfolded proteins and controlling the targeting of proteins for subsequent degradation. This is achieved through cycles of ATP binding, ATP hydrolysis and ADP release, mediated by co-chaperones. The affinity for polypeptides is regulated by its nucleotide bound state. In the ATP-bound form, it has a low affinity for substrate proteins. However, upon hydrolysis of the ATP to ADP, it undergoes a conformational change that increases its affinity for substrate proteins. It goes through repeated cycles of ATP hydrolysis and nucleotide exchange, which permits cycles of substrate binding and release (PubMed:26865365).

Positive regulator of PRKN translocation to damaged mitochondria (PubMed:24270810).

Biological Process

Binding of sperm to zona pellucida Source: Ensembl
Cellular response to unfolded protein Source: GO_Central
Chaperone cofactor-dependent protein refolding Source: GO_Central
Positive regulation of protein targeting to mitochondrion Source: ParkinsonsUK-UCL
Protein refolding Source: UniProtKB
Response to unfolded protein Source: ProtInc
Vesicle-mediated transport Source: GO_Central

Cellular Location

Cytosol; Blood microparticle; Mitochondrial matrix; Nucleoplasm; Nucleus; Plasma membrane; Cell body; Cytoplasm; Zona pellucida receptor complex

Liu, X., Teng, Z., Wang, Z., Zhu, P., Song, Z., & Liu, F. (2022). Expressions of HSPA1L and HSPA9 are associated with poor sperm quality of low‐motility spermatozoa in fertile men. Andrologia, 54(2), e14321.

Muhammad, J. S., Saheb Sharif-Askari, N., Cui, Z. G., Hamad, M., & Halwani, R. (2021). SARS-CoV-2 infection-induced promoter hypomethylation as an epigenetic modulator of heat shock protein A1L (HSPA1L) gene. Frontiers in Genetics, 12, 622271.

Nakatsuka, A., Yamaguchi, S., Eguchi, J., Kakuta, S., Iwakura, Y., Sugiyama, H., & Wada, J. (2021). A Vaspin–HSPA1L complex protects proximal tubular cells from organelle stress in diabetic kidney disease. Communications Biology, 4(1), 373.

Lee, J. H., Yoon, Y. M., Song, K. H., Noh, H., & Lee, S. H. (2020). Melatonin suppresses senescence‐derived mitochondrial dysfunction in mesenchymal stem cells via the HSPA1L–mitophagy pathway. Aging cell, 19(3), e13111.

Wang, X., Xie, W., Yao, Y., Zhu, Y., Zhou, J., Cui, Y., ... & Liu, M. (2020). The heat shock protein family gene Hspa1l in male mice is dispensable for fertility. PeerJ, 8, e8702.

De Andrade, W. P., Braga, L. D. C., Goncales, N. G., Silva, L. M., & Da Silva Filho, A. L. (2020). HSPA1A, HSPA1L and TRAP1 heat shock genes may be associated with prognosis in ovarian epithelial cancer. Oncology Letters, 19(1), 359-367.

Choi, S. I., Lee, J. H., Kim, R. K., Jung, U., Kahm, Y. J., Cho, E. W., & Kim, I. G. (2020). HSPA1L enhances cancer stem cell-like properties by activating IGF1Rβ and regulating β-catenin transcription. International Journal of Molecular Sciences, 21(18), 6957.

Williams, P. A., Kobilnyk, H. E., McMillan, E. A., & Strochlic, T. I. (2019). MAPKAP kinase 2–mediated phosphorylation of HspA1L protects male germ cells from heat stress–induced apoptosis. Cell Stress and Chaperones, 24, 1127-1136.

Kohan, L., Tabiee, O., & Sepahi, N. (2019). HSPA1L and HSPA1B gene polymorphisms and haplotypes are associated with idiopathic male infertility in Iranian population. European Journal of Obstetrics & Gynecology and Reproductive Biology, 240, 57-61.

Huusko, J. M., Karjalainen, M. K., Graham, B. E., Zhang, G., Farrow, E. G., Miller, N. A., ... & Muglia, L. J. (2018). Whole exome sequencing reveals HSPA1L as a genetic risk factor for spontaneous preterm birth. PLoS genetics, 14(7), e1007394.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Antibody Pairs

HSPA1L Matched Antibody Pair (576) (CAT#: APMAB-576LY)

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Isotype control

For research use only. Not intended for any clinical use.

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