Rabbit Anti-HSPD1 Recombinant Antibody (
3D8) (V2LY-0725-LY1522)

Name: Rabbit Anti-HSPD1 Recombinant Antibody (3D8)
SKU: V2LY-0725-LY1522
Rating: 5 (1 reviews)

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Tested Data

Rabbit Anti-HSPD1 Recombinant Antibody (3D8) (V2LY-0725-LY1522) in WB

Western Blot
Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, 293 whole cell lysate, Jurkat whole cell lysate, MCF-7 whole cell lysate, K562 whole cell lysate, Mouse brain tissue
All lanes: HSPD1 antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 62, 18 kDa
Observed band size: 60 kDa

Rabbit Anti-HSPD1 Recombinant Antibody (3D8) (V2LY-0725-LY1522) in IHC

IHC image of V2LY-0725-LY1522 diluted at 1:100 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Rabbit Anti-HSPD1 Recombinant Antibody (3D8) (V2LY-0725-LY1522) in IHC

IHC image of V2LY-0725-LY1522 diluted at 1:100 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Rabbit Anti-HSPD1 Recombinant Antibody (3D8) (V2LY-0725-LY1522) in IP

Immunoprecipitating HSPD1 in HepG2 whole cell lysate
Lane 1: Rabbit control IgG instead of V2LY-0725-LY1522 in HepG2 whole cell lysate.
For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: V2LY-0725-LY1522(3µg)+ HepG2 whole cell lysate(500µg)
Lane 3: HepG2 whole cell lysate (10µg)

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

3D8

Application

ELISA, WB, IHC, IP

Immunogen

A synthesized peptide derived from human Hsp60.

Host Species

Rabbit

Specificity

Human, Mouse

Antibody Isotype

IgG

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:5,000
IHC	1:50-1:200
IP	1:200-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, glycerol

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

heat shock protein family D (Hsp60) member 1

Entrez Gene ID

3329

UniProt ID

P10809

Function

Chaperonin implicated in mitochondrial protein import and macromolecular assembly. Together with Hsp10, facilitates the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix (PubMed:1346131, PubMed:11422376).

The functional units of these chaperonins consist of heptameric rings of the large subunit Hsp60, which function as a back-to-back double ring. In a cyclic reaction, Hsp60 ring complexes bind one unfolded substrate protein per ring, followed by the binding of ATP and association with 2 heptameric rings of the co-chaperonin Hsp10. This leads to sequestration of the substrate protein in the inner cavity of Hsp60 where, for a certain period of time, it can fold undisturbed by other cell components. Synchronous hydrolysis of ATP in all Hsp60 subunits results in the dissociation of the chaperonin rings and the release of ADP and the folded substrate protein (Probable).

Biological Process

de novo' protein folding Source: BHF-UCL
Activation of cysteine-type endopeptidase activity involved in apoptotic process Source: BHF-UCL
Apoptotic mitochondrial changes Source: GO_Central
B cell activation Source: BHF-UCL
B cell proliferation Source: BHF-UCL
Biological process involved in interaction with symbiont Source: CAFA
Chaperone-mediated protein complex assembly Source: BHF-UCL
Isotype switching to IgG isotypes Source: BHF-UCL
Mitochondrial unfolded protein response Source: GO_Central
MyD88-dependent toll-like receptor signaling pathway Source: BHF-UCL
Negative regulation of apoptotic process Source: UniProtKB
Positive regulation of apoptotic process Source: BHF-UCL
Positive regulation of interferon-alpha production Source: BHF-UCL
Positive regulation of interferon-gamma production Source: BHF-UCL
Positive regulation of interleukin-10 production Source: BHF-UCL
Positive regulation of interleukin-12 production Source: BHF-UCL
Positive regulation of interleukin-6 production Source: BHF-UCL
Positive regulation of macrophage activation Source: BHF-UCL
Positive regulation of T cell activation Source: BHF-UCL
Positive regulation of T cell mediated immune response to tumor cell Source: BHF-UCL
Protein folding Source: GO_Central
Protein import into mitochondrial intermembrane space Source: GO_Central
Protein maturation Source: BHF-UCL
Protein refolding Source: UniProtKB
Protein stabilization Source: UniProtKB
Response to cold Source: AgBase
Response to unfolded protein Source: BHF-UCL
T cell activation Source: MGI

Cellular Location

Mitochondrion matrix

Involvement in disease

Spastic paraplegia 13, autosomal dominant (SPG13):
A form of spastic paraplegia, a neurodegenerative disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs. Rate of progression and the severity of symptoms are quite variable. Initial symptoms may include difficulty with balance, weakness and stiffness in the legs, muscle spasms, and dragging the toes when walking. In some forms of the disorder, bladder symptoms (such as incontinence) may appear, or the weakness and stiffness may spread to other parts of the body.
Leukodystrophy, hypomyelinating, 4 (HLD4):
A severe autosomal recessive hypomyelinating leukodystrophy. Clinically characterized by infantile-onset rotary nystagmus, progressive spastic paraplegia, neurologic regression, motor impairment, profound mental retardation. Death usually occurs within the first two decades of life.

Duan, Y., Yu, J., Chen, M., Lu, Q., Ning, F., Gan, X., ... & Lash, G. E. (2023). Knockdown of heat shock protein family D member 1 (HSPD1) promotes proliferation and migration of ovarian cancer cells via disrupting the stability of mitochondrial 3-oxoacyl-ACP synthase (OXSM). Journal of ovarian research, 16(1), 1-14.

Zheng, X., Chang, S., Liu, Y., Dai, X., & You, C. (2023). Human Mitochondrial Protein HSPD1 Binds to and Regulates the Repair of Deoxyinosine in DNA. Journal of Proteome Research, 22(4), 1339-1346.

Wu, T., Jia, L., Lei, S., Jiang, H., Liu, J., Li, N., ... & Lei, L. (2022). Host HSPD1 Translocation from Mitochondria to the Cytoplasm Induced by Streptococcus suis Serovar 2 Enolase Mediates Apoptosis and Loss of Blood–Brain Barrier Integrity. Cells, 11(13), 2071.

Song, X., Chen, B., Liang, Y., Li, Y., Zhang, H., Han, D., ... & Yang, Q. (2022). CircEIF3H-IGF2BP2-HuR scaffold complex promotes TNBC progression via stabilizing HSPD1/RBM8A/G3BP1 mRNA. Cell Death Discovery, 8(1), 261.

Parma, B., Ramesh, V., Gollavilli, P. N., Siddiqui, A., Pinna, L., Schwab, A., ... & Ceppi, P. (2021). Metabolic impairment of non-small cell lung cancers by mitochondrial HSPD1 targeting. Journal of Experimental & Clinical Cancer Research, 40(1), 1-20.

Klebl, D. P., Feasey, M. C., Hesketh, E. L., Ranson, N. A., Wurdak, H., Sobott, F., ... & Muench, S. P. (2021). Cryo-EM structure of human mitochondrial HSPD1. Iscience, 24(1).

Albadawy, R., Agwa, S. H., Khairy, E., Saad, M., El Touchy, N., Othman, M., & Matboli, M. (2021). Clinical significance of hspd1/mmp14/itgb1/mir-6881-5p/lnc-sparcl1-1: 2 rna panel in nafld/nash diagnosis: Egyptian pilot study. Biomedicines, 9(9), 1248.

Cömert, C., Brick, L., Ang, D., Palmfeldt, J., Meaney, B. F., Kozenko, M., ... & Bross, P. (2020). A recurrent de novo HSPD1 variant is associated with hypomyelinating leukodystrophy. Molecular Case Studies, 6(3), a004879.

Kang, B. H., Shu, C. W., Chao, J. K., Lee, C. H., Fu, T. Y., Liou, H. H., ... & Liu, P. F. (2019). HSPD1 repressed E-cadherin expression to promote cell invasion and migration for poor prognosis in oral squamous cell carcinoma. Scientific reports, 9(1), 8932.

Kim, S. K., Kim, K., Ryu, J. W., Ryu, T. Y., Lim, J. H., Oh, J. H., ... & Cho, H. S. (2019). The novel prognostic marker, EHMT2, is involved in cell proliferation via HSPD1 regulation in breast cancer. International Journal of Oncology, 54(1), 65-76.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Antibody Pairs

HSPD1 Matched Antibody Pair (578) (CAT#: APMAB-578LY)

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For research use only. Not intended for any clinical use.

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