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Mouse Anti-HTR5A Recombinant Antibody (10D3) (CBMAB-A4155-LY)

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Datasheet
SDS
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Basic Information

Host Animal
Mouse
Clone
10D3
Application
WB, ELISA
Immunogen
HTR5A (NP_076917, 223 a.a. ~ 282 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.
More Infomation

Target

Full Name
5-Hydroxytryptamine Receptor 5A
Introduction
The neurotransmitter serotonin (5-hydroxytryptamine, 5-HT) has been implicated in a wide range of psychiatric conditions and also has vasoconstrictive and vasodilatory effects. The gene described in this record is a member of 5-hydroxytryptamine (serotonin) receptor family and encodes a multi-pass membrane protein that functions as a receptor for 5-hydroxytryptamine and couples to G-proteins. This protein has been shown to function in part through the regulation of intracellular Ca2+ mobilization. [provided by RefSeq]
Entrez Gene ID
3361
UniProt ID
P47898
Alternative Names
5-HT5A; MGC138226
Function
This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. The activity of this receptor is mediated by G proteins.
Biological Process
Adenylate cyclase-activating G protein-coupled receptor signaling pathway Source: Ensembl
Adenylate cyclase-inhibiting serotonin receptor signaling pathway Source: GO_Central
Chemical synaptic transmission Source: GO_Central
G protein-coupled receptor signaling pathway Source: ProtInc
G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger Source: GO_Central
Hippocampus development Source: Ensembl
Response to estradiol Source: Ensembl
Cellular Location
Cell membrane
Topology
Extracellular: 1-40
Helical: 41-63
Cytoplasmic: 64-78
Helical: 79-99
Extracellular: 100-115
Helical: 116-137
Cytoplasmic: 138-158
Helical: 159-181
Extracellular: 182-198
Helical: 199-219
Cytoplasmic: 220-282
Helical: 283-303
Extracellular: 304-320
Helical: 321-341
Cytoplasmic: 342-357

Priyadarshini, S., Pradhan, B., Griebel, P., & Aich, P. (2018). Cortisol regulates immune and metabolic processes in murine adipocytes and macrophages through HTR2c and HTR5a serotonin receptors. European Journal of Cell Biology, 97(7), 483-492.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Mouse Anti-HTR5A Recombinant Antibody (3D1) (CAT#: CBMAB-A4156-LY)

Rabbit Anti-HTR5A Recombinant Antibody (EG1593) (CAT#: CBMAB-EN1906-LY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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