Rabbit Anti-HUS1 Recombinant Antibody (D4J9H) (CBMAB-CP1083-LY)

Name: Rabbit Anti-HUS1 Recombinant Antibody (D4J9H)
SKU: CBMAB-CP1083-LY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

D4J9H

Application

WB, IP

Immunogen

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln147 of human HUS1 protein.

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

100 µg/ml BSA, 50% glycerol

Preservative

0.02% sodium azide

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

HUS1 Checkpoint Clamp Component

Introduction

The protein encoded by this gene is a component of an evolutionarily conserved, genotoxin-activated checkpoint complex that is involved in the cell cycle arrest in response to DNA damage. This protein forms a heterotrimeric complex with checkpoint proteins RAD9 and RAD1. In response to DNA damage, the trimeric complex interacts with another protein complex consisting of checkpoint protein RAD17 and four small subunits of the replication factor C (RFC), which loads the combined complex onto the chromatin. The DNA damage induced chromatin binding has been shown to depend on the activation of the checkpoint kinase ATM, and is thought to be an early checkpoint signaling event. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2011]

Entrez Gene ID

3364

UniProt ID

O60921

Alternative Names

HUS1 Checkpoint Clamp Component; Hus1+-Like Protein; HHUS1; HUS1 (S. Pombe) Checkpoint Homolog; HUS1 Checkpoint Homolog (S. Pombe); HUS1 Checkpoint Homolog; Checkpoint Protein HUS1;

Function

Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates. The 9-1-1 complex is necessary for the recruitment of RHNO1 to sites of double-stranded breaks (DSB) occurring during the S phase.

Biological Process

Cellular response to DNA damage stimulus Source: ProtInc
Cellular response to ionizing radiation Source: UniProtKB
DNA damage checkpoint signaling Source: UniProtKB
DNA repair Source: ProtInc
Double-strand break repair via homologous recombination Source: GO_Central
Embryo development ending in birth or egg hatching Source: Ensembl
Meiotic DNA integrity checkpoint signaling Source: GO_Central
Mitotic DNA replication checkpoint signaling Source: GO_Central
Mitotic intra-S DNA damage checkpoint signaling Source: GO_Central
Nucleotide-excision repair Source: GO_Central
Protein phosphorylation Source: Ensembl
Regulation of protein phosphorylation Source: Ensembl
Response to UV Source: Ensembl
Telomere maintenance Source: GO_Central

Cellular Location

Cytosol; Nucleus. In discrete nuclear foci upon DNA damage. According to PubMed:11077446, localized also in the cytoplasm. DNA damage induces its nuclear translocation. Shuttles between the nucleus and the cytoplasm.

Mascarenhas dos Santos, A. C., Julian, A. T., & Pombert, J. F. (2022). The Rad9–Rad1–Hus1 DNA Repair clamp is found in microsporidia. Genome Biology and Evolution, 14(4), evac053.

Hwang, B. J., Gonzales, R., Corzine, S., Stenson, E., Pidugu, L., & Lu, A. L. (2022). DNA binding by the Rad9A subunit of the Rad9-Rad1-Hus1 complex. Plos one, 17(8), e0272645.

Hashimoto, H., Hara, K., & Hishiki, A. (2022). Structural basis for molecular interactions on the eukaryotic DNA sliding clamps PCNA and RAD9-RAD1-HUS1. The Journal of Biochemistry, 172(4), 189-196.

Ren, K., Yu, Y., Wang, X., Liu, H., & Zhao, J. (2021). MiR-340-3p-HUS1 axis suppresses proliferation and migration in lung adenocarcinoma cells. Life Sciences, 274, 119330.

Tan, J., Wang, X., Hwang, B. J., Gonzales, R., Konen, O., Lan, L., & Lu, A. L. (2020). An ordered assembly of MYH glycosylase, SIRT6 protein deacetylase, and Rad9-Rad1-Hus1 checkpoint clamp at oxidatively damaged telomeres. Aging (Albany NY), 12(18), 17761.

Zhou, Z. Q., Zhao, J. J., Chen, C. L., Liu, Y., Zeng, J. X., Wu, Z. R., ... & Xia, J. C. (2019). HUS1 checkpoint clamp component (HUS1) is a potential tumor suppressor in primary hepatocellular carcinoma. Molecular Carcinogenesis, 58(1), 76-87.

Damasceno, J. D., Obonaga, R., Silva, G. L., Reis-Cunha, J. L., Duncan, S. M., Bartholomeu, D. C., ... & Tosi, L. R. (2018). Conditional genome engineering reveals canonical and divergent roles for the Hus1 component of the 9–1–1 complex in the maintenance of the plastic genome of Leishmania. Nucleic Acids Research, 46(22), 11835-11846.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Antibody Pairs

HUS1 Matched Antibody Pair (579) (CAT#: APMAB-579LY)

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HUS1 Matched Antibody Pair (579) (CAT#: APMAB-579LY)

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Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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