Sign in or Register   Sign in or Register
  |  

Rabbit Anti-HUS1 Recombinant Antibody (D4J9H) (CBMAB-CP1083-LY)

The product is antibody recognizes HUS1. The antibody D4J9H immunoassay techniques such as: WB,IP.
See all HUS1 antibodies

Summary

Host Animal
Rabbit
Specificity
Human
Clone
D4J9H
Antibody Isotype
IgG
Application
WB, IP

Basic Information

Immunogen
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln147 of human HUS1 protein.
Specificity
Human
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
100 µg/ml BSA, 50% glycerol
Preservative
0.02% sodium azide
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
HUS1 Checkpoint Clamp Component
Introduction
The protein encoded by this gene is a component of an evolutionarily conserved, genotoxin-activated checkpoint complex that is involved in the cell cycle arrest in response to DNA damage. This protein forms a heterotrimeric complex with checkpoint proteins RAD9 and RAD1. In response to DNA damage, the trimeric complex interacts with another protein complex consisting of checkpoint protein RAD17 and four small subunits of the replication factor C (RFC), which loads the combined complex onto the chromatin. The DNA damage induced chromatin binding has been shown to depend on the activation of the checkpoint kinase ATM, and is thought to be an early checkpoint signaling event. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2011]
Entrez Gene ID
3364
UniProt ID
O60921
Alternative Names
HUS1 Checkpoint Clamp Component; Hus1+-Like Protein; HHUS1; HUS1 (S. Pombe) Checkpoint Homolog; HUS1 Checkpoint Homolog (S. Pombe); HUS1 Checkpoint Homolog; Checkpoint Protein HUS1;
Function
Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates. The 9-1-1 complex is necessary for the recruitment of RHNO1 to sites of double-stranded breaks (DSB) occurring during the S phase.
Biological Process
Cellular response to DNA damage stimulus Source: ProtInc
Cellular response to ionizing radiation Source: UniProtKB
DNA damage checkpoint signaling Source: UniProtKB
DNA repair Source: ProtInc
Double-strand break repair via homologous recombination Source: GO_Central
Embryo development ending in birth or egg hatching Source: Ensembl
Meiotic DNA integrity checkpoint signaling Source: GO_Central
Mitotic DNA replication checkpoint signaling Source: GO_Central
Mitotic intra-S DNA damage checkpoint signaling Source: GO_Central
Nucleotide-excision repair Source: GO_Central
Protein phosphorylation Source: Ensembl
Regulation of protein phosphorylation Source: Ensembl
Response to UV Source: Ensembl
Telomere maintenance Source: GO_Central
Cellular Location
Cytosol; Nucleus. In discrete nuclear foci upon DNA damage. According to PubMed:11077446, localized also in the cytoplasm. DNA damage induces its nuclear translocation. Shuttles between the nucleus and the cytoplasm.

Mascarenhas dos Santos, A. C., Julian, A. T., & Pombert, J. F. (2022). The Rad9–Rad1–Hus1 DNA Repair clamp is found in microsporidia. Genome Biology and Evolution, 14(4), evac053.

Hwang, B. J., Gonzales, R., Corzine, S., Stenson, E., Pidugu, L., & Lu, A. L. (2022). DNA binding by the Rad9A subunit of the Rad9-Rad1-Hus1 complex. Plos one, 17(8), e0272645.

Hashimoto, H., Hara, K., & Hishiki, A. (2022). Structural basis for molecular interactions on the eukaryotic DNA sliding clamps PCNA and RAD9-RAD1-HUS1. The Journal of Biochemistry, 172(4), 189-196.

Ren, K., Yu, Y., Wang, X., Liu, H., & Zhao, J. (2021). MiR-340-3p-HUS1 axis suppresses proliferation and migration in lung adenocarcinoma cells. Life Sciences, 274, 119330.

Tan, J., Wang, X., Hwang, B. J., Gonzales, R., Konen, O., Lan, L., & Lu, A. L. (2020). An ordered assembly of MYH glycosylase, SIRT6 protein deacetylase, and Rad9-Rad1-Hus1 checkpoint clamp at oxidatively damaged telomeres. Aging (Albany NY), 12(18), 17761.

Zhou, Z. Q., Zhao, J. J., Chen, C. L., Liu, Y., Zeng, J. X., Wu, Z. R., ... & Xia, J. C. (2019). HUS1 checkpoint clamp component (HUS1) is a potential tumor suppressor in primary hepatocellular carcinoma. Molecular Carcinogenesis, 58(1), 76-87.

Damasceno, J. D., Obonaga, R., Silva, G. L., Reis-Cunha, J. L., Duncan, S. M., Bartholomeu, D. C., ... & Tosi, L. R. (2018). Conditional genome engineering reveals canonical and divergent roles for the Hus1 component of the 9–1–1 complex in the maintenance of the plastic genome of Leishmania. Nucleic Acids Research, 46(22), 11835-11846.

Ask a question We look forward to hearing from you.
0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Loading...
Have you used Rabbit Anti-HUS1 Recombinant Antibody (D4J9H)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon $30 eGift Card
Submit a review
Loading...
For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Documents

Contact us

  • Tel: (USA)
  • (UK)
  • Fax:
  • Email:

Submit A Review

Go to
Compare