Rabbit Anti-ID1 Recombinant Antibody (
2C7) (V2LY-0725-LY1119)

Name: Rabbit Anti-ID1 Recombinant Antibody (2C7)
SKU: V2LY-0725-LY1119
Rating: 5 (1 reviews)

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Tested Data

Rabbit Anti-ID1 Recombinant Antibody (2C7) (V2LY-0725-LY1119) in WB

Western Blot
Positive WB detected in: 293T whole cell lysate, Hela whole cell lysate, HepG2 whole cell lysate 293 whole cell lysate, A549 whole cell lysate, U-251 whole cell lysate, Mouse Brain whole cell lysate
All lanes: Id1 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 17, 16 kDa
Observed band size: 26 kDa

Rabbit Anti-ID1 Recombinant Antibody (2C7) (V2LY-0725-LY1119) in IF

Immunofluorescence staining of Hela Cells with V2LY-0725-LY1119 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

2C7

Application

ELISA, WB, IF

Immunogen

A synthesized peptide derived from human Id1.

Host Species

Rabbit

Specificity

Human, Mouse

Antibody Isotype

IgG

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:5,000
IF	1:20-1:200

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, glycerol

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

ID1

Entrez Gene ID

3397

UniProt ID

P41134

Function

Transcriptional regulator (lacking a basic DNA binding domain) which negatively regulates the basic helix-loop-helix (bHLH) transcription factors by forming heterodimers and inhibiting their DNA binding and transcriptional activity. Implicated in regulating a variety of cellular processes, including cellular growth, senescence, differentiation, apoptosis, angiogenesis, and neoplastic transformation. Inhibits skeletal muscle and cardiac myocyte differentiation. Regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-ARNTL/BMAL1 heterodimer (By similarity).

Biological Process

Angiogenesis Source: BHF-UCL
Blood vessel endothelial cell migration Source: BHF-UCL
Blood vessel morphogenesis Source: BHF-UCL
Brain development Source: Ensembl
Cell-abiotic substrate adhesion Source: Ensembl
Cell differentiation Source: GO_Central
Cellular response to dopamine Source: Ensembl
Cellular response to epidermal growth factor stimulus Source: Ensembl
Cellular response to nerve growth factor stimulus Source: Ensembl
Cellular response to peptide Source: Ensembl
Circadian regulation of gene expression Source: GO_Central
Negative regulation of apoptotic process Source: Ensembl
Negative regulation of cold-induced thermogenesis Source: YuBioLab
Negative regulation of dendrite morphogenesis Source: Ensembl
Negative regulation of DNA binding Source: Ensembl
Negative regulation of DNA-binding transcription factor activity Source: ARUK-UCL
Negative regulation of endothelial cell differentiation Source: Ensembl
Negative regulation of protein binding Source: Ensembl
Negative regulation of transcription, DNA-templated Source: GDB
Negative regulation of transcription by RNA polymerase II Source: ARUK-UCL
Negative regulation of transcription by transcription factor localization Source: BHF-UCL
Neuron differentiation Source: Ensembl
Positive regulation of actin filament bundle assembly Source: Ensembl
Positive regulation of epithelial cell proliferation Source: Ensembl
Positive regulation of gene expression Source: Ensembl
Regulation of vasculature development Source: Ensembl
Transforming growth factor beta receptor signaling pathway Source: BHF-UCL

Cellular Location

Cytoplasm; Nucleus

Lin, Z., Liu, Y., Xu, T., Su, T., Yang, Y., Liang, R., ... & Xie, L. (2023). STAT3-Mediated Promoter-Enhancer Interaction Up-Regulates Inhibitor of DNA Binding 1 (ID1) to Promote Colon Cancer Progression. International Journal of Molecular Sciences, 24(12), 10041.

Wei, J., Shi, Y., Zou, C., Zhang, H., Peng, H., Wang, S., ... & Wang, D. (2022). Cellular Id1 inhibits hepatitis B virus transcription by interacting with the novel covalently closed circular DNA-binding protein E2F4. International Journal of Biological Sciences, 18(1), 65.

Zuniga, O., Byrum, S., & Wolfe, A. R. (2022). Discovery of the inhibitor of DNA binding 1 as a novel marker for radioresistance in pancreatic cancer using genome-wide RNA-seq. Cancer Drug Resistance, 5(4), 926.

Zhao, Z., Bo, Z., Gong, W., & Guo, Y. (2020). Inhibitor of differentiation 1 (Id1) in cancer and cancer therapy. International journal of medical sciences, 17(8), 995.

Leng, X., Ji, X., Hou, Y., Settlage, R., & Jiang, H. (2019). Roles of the proteasome and inhibitor of DNA binding 1 protein in myoblast differentiation. The FASEB Journal, 33(6), 7403-7416.

Sun, Y., Lai, X., Yu, Y., Li, J., Cao, L., Lin, W., ... & Ye, Y. (2019). Inhibitor of DNA binding 1 (Id1) mediates stemness of colorectal cancer cells through the Id1-c-Myc-PLAC8 axis via the Wnt/β-catenin and Shh signaling pathways. Cancer management and research, 6855-6869.

Ohara, R. A., Edhayan, G., Rasmussen, S. M., Isozaki, T., Remmer, H. A., Lanigan, T. M., ... & Ruth, J. H. (2019). Citrullinated inhibitor of DNA binding 1 is a novel autoantigen in rheumatoid arthritis. Arthritis & Rheumatology, 71(8), 1241-1251.

Zhou, X. L., Zeng, D. E., Ye, Y. H., Sun, S. M., Lu, X. F., Liang, W. Q., ... & Lin, H. Y. (2018). Prognostic values of the inhibitor of DNA‑binding family members in breast cancer. Oncology Reports, 40(4), 1897-1906.

Han, J., Seo, H., Choi, Y., Lee, C., Kim, M. I., Jeon, Y., ... & Ka, H. (2018). Expression and regulation of inhibitor of DNA binding proteins ID1, ID2, ID3, and ID4 at the maternal-conceptus interface in pigs. Theriogenology, 108, 46-55.

Ke, J., Wu, R., Chen, Y., & Abba, M. L. (2018). Inhibitor of DNA binding proteins: implications in human cancer progression and metastasis. American Journal of Translational Research, 10(12), 3887.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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