Mouse Anti-IER2 Recombinant Antibody (3E2) (CBMAB-A4234-LY)

The product is antibody recognizes IER2. The antibody 3E2 immunoassay techniques such as: WB, ELISA.
See all IER2 antibodies

Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

3E2

Antibody Isotype

IgG2a, κ

Application

WB, ELISA

Basic Information

Immunogen

IER2 (AAH03625, 1 a.a. ~ 223 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name

immediate early response 2

Entrez Gene ID

9592

UniProt ID

Q9BTL4

Alternative Names

ETR101; MGC111472; MGC15265

Function

DNA-binding protein that seems to act as a transcription factor (PubMed:19584537).

Involved in the regulation of neuronal differentiation, acts upon JNK-signaling pathway activation and plays a role in neurite outgrowth in hippocampal cells (By similarity).

May mediate with FIBP FGF-signaling in the establishment of laterality in the embryo (By similarity).

Promotes cell motility, seems to stimulate tumor metastasis (PubMed:22120713).

Biological Process

Cell motility Source: UniProtKB
Neuron differentiation Source: UniProtKB
Positive regulation of transcription by RNA polymerase II Source: NTNU_SB
Response to fibroblast growth factor Source: UniProtKB

Cellular Location

Cytoplasm; Nucleus. Cytoplasmic during quiescence, translocates to the nucleus upon stimulation.

References

Doi, K., Takeuchi, H., & Sakurai, H. (2023). PP2A‐B55 and its adapter proteins IER2 and IER5 regulate the activity of RB family proteins and the expression of cell cycle‐related genes. The FEBS Journal, 290(3), 745-762.

Kyjacova, L., Saup, R., Rönsch, K., Wallbaum, S., Dukowic-Schulze, S., Foss, A., ... & Sleeman, J. P. (2021). IER2-induced senescence drives melanoma invasion through osteopontin. Oncogene, 40(47), 6494-6512.

Xu, X., Zhou, W., Chen, Y., Wu, K., Wang, H., Zhang, J., ... & Shen, W. (2020). Immediate early response protein 2 promotes the migration and invasion of hepatocellular carcinoma cells via regulating the activity of Rho GTPases. Neoplasma, 67(3), 614-622.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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Documents

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SDS
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