Rabbit Anti-IFIT5 Recombinant Antibody (EG1609) (CBMAB-EN1923-LY)

Name: Rabbit Anti-IFIT5 Recombinant Antibody (EG1609)
SKU: CBMAB-EN1923-LY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

EG1609

Application

WB: 1:500~1:1000 ELISA: 1:40000

Immunogen

The antibody was produced against synthesized peptide derived from internal of human IFIT5.

Specificity

Human

Antibody Isotype

IgG

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Interferon Induced Protein With Tetratricopeptide Repeats 5

Introduction

IFIT5 (Interferon Induced Protein With Tetratricopeptide Repeats 5) is a Protein Coding gene. Gene Ontology (GO) annotations related to this gene include tRNA binding. An important paralog of this gene is IFIT1B.

Entrez Gene ID

24138

UniProt ID

Q13325

Alternative Names

Interferon Induced Protein With Tetratricopeptide Repeats 5

Function

Interferon-induced RNA-binding protein involved in the human innate immune response. Has a broad and adaptable RNA structure recognition important for RNA recognition specificity in antiviral defense. Binds precursor and processed tRNAs as well as poly-U-tailed tRNA fragments (PubMed:25092312, PubMed:23317505, PubMed:23774268).

Specifically binds single-stranded RNA bearing a 5'-triphosphate group (PPP-RNA), thereby acting as a sensor of viral single-stranded RNAs. Single-stranded PPP-RNAs, which lack 2'-O-methylation of the 5' cap and bear a 5'-triphosphate group instead, are specific from viruses, providing a molecular signature to distinguish between self and non-self mRNAs by the host during viral infection. Directly binds PPP-RNA in a non-sequence-specific manner (PubMed:23334420).

Also recognizes and selectively binds AT-rich dsDNA (PubMed:23774268).

Additionally, as a mediator in innate immunity, regulates positively IKK-NFKB signaling by sinergizing the recruitment of IKK to MAP3K7 (PubMed:26334375).

Biological Process

Defense response to virus Source: UniProtKB
Innate immune response Source: UniProtKB-KW
Negative regulation of viral genome replication Source: UniProtKB
Positive regulation of I-kappaB kinase/NF-kappaB signaling Source: UniProtKB

Cellular Location

Ruffle membrane. Colocalized with DDX58/RIG-I at cell surface ruffles. Localizes to actin-rich protrusions from the apical cell surface.

Budniak, U. A., Karolak, N. K., Kulik, M., Młynarczyk, K., Górna, M. W., & Dominiak, P. M. (2022). The Role of Electrostatic Interactions in IFIT5-RNA Complexes Predicted by the UBDB+ EPMM Method. The Journal of Physical Chemistry B, 126(45), 9152-9167.

Wu, Y., Song, X., Cui, D., & Zhang, T. (2022). IFIT3 and IFIT5 play potential roles in innate immune response of porcine pulmonary microvascular endothelial cells to highly pathogenic porcine reproductive and respiratory syndrome virus. Viruses, 14(9), 1919.

Wang, S., Wan, L., Ren, H., Xie, Z., Xie, L., Huang, J., ... & Zhang, M. (2022). Screening of interferon-stimulated genes against avian reovirus infection and mechanistic exploration of the antiviral activity of IFIT5. Frontiers in Microbiology, 13, 998505.

Li, J. J., Yin, Y., Yang, H. L., Yang, C. W., Yu, C. L., Wang, Y., ... & Liu, Y. P. (2020). mRNA expression and functional analysis of chicken IFIT5 after infected with Newcastle disease virus. Infection, Genetics and Evolution, 86, 104585.

Miedziak, B., Dobieżyńska, A., Darżynkiewicz, Z. M., Bartkowska, J., Miszkiewicz, J., Kowalska, J., ... & Grzela, R. (2020). Kinetic analysis of IFIT1 and IFIT5 interactions with different native and engineered RNAs and its consequences for designing mRNA-based therapeutics. RNA, 26(1), 58-68.

Lo, U. G., Bao, J., Cen, J., Yeh, H. C., Luo, J., Tan, W., & Hsieh, J. T. (2019). Interferon-induced IFIT5 promotes epithelial-to-mesenchymal transition leading to renal cancer invasion. American journal of clinical and experimental urology, 7(1), 31.

Lo, U. G., Pong, R. C., Yang, D., Gandee, L., Hernandez, E., Dang, A., ... & Hsieh, J. T. (2019). IFNγ-Induced IFIT5 promotes epithelial-to-mesenchymal transition in prostate cancer via miRNA processing. Cancer research, 79(6), 1098-1112.

Huang, J., Lo, U. G., Wu, S., Wang, B., Pong, R. C., Lai, C. H., ... & Wu, K. (2019). The roles and mechanism of IFIT5 in bladder cancer epithelial–mesenchymal transition and progression. Cell death & disease, 10(6), 437.

Chico, V., Salvador-Mira, M. E., Nombela, I., Puente-Marin, S., Ciordia, S., Mena, M. C., ... & Ortega-Villaizan, M. D. M. (2019). Ifit5 participates in the antiviral mechanisms of rainbow trout red blood cells. Frontiers in immunology, 10, 613.

Rohaim, M. A., Santhakumar, D., Naggar, R. F. E., Iqbal, M., Hussein, H. A., & Munir, M. (2018). Chickens expressing IFIT5 ameliorate clinical outcome and pathology of highly pathogenic avian influenza and velogenic newcastle disease viruses. Frontiers in immunology, 9, 2025.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

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