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Mouse Anti-IFITM2 Recombinant Antibody (CF213) (CBMAB-FT256LY)


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Summary

Host Animal
Mouse
Specificity
Human
Clone
CF213
Antibody Isotype
IgG1
Application
ELISA, WB, FC

Basic Information

Immunogen
interferon induced transmembrane protein 2
Specificity
Human
Antibody Isotype
IgG1
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
50% glycerol
Preservative
0.02% sodium azide
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Interferon Induced Transmembrane Protein 2
Introduction
IFITM2 (Interferon Induced Transmembrane Protein 2) is a Protein Coding gene. Diseases associated with IFITM2 include Influenza and Dengue Virus. Among its related pathways are Cytokine Signaling in Immune system and Innate Immune System. An important paralog of this gene is IFITM3.
Entrez Gene ID
10581
UniProt ID
Q01629
Alternative Names
DSPA2c, IFITM2
Function
IFN-induced antiviral protein which inhibits the entry of viruses to the host cell cytoplasm, permitting endocytosis, but preventing subsequent viral fusion and release of viral contents into the cytosol (PubMed:33563656, PubMed:26354436).

Active against multiple viruses, including influenza A virus, SARS coronaviruses (SARS-CoV and SARS-CoV-2), Marburg virus (MARV), Ebola virus (EBOV), Dengue virus (DNV), West Nile virus (WNV), human immunodeficiency virus type 1 (HIV-1), hepatitis C virus (HCV) and vesicular stomatitis virus (VSV) (PubMed:33563656, PubMed:26354436, PubMed:33270927, PubMed:33239446).

Can inhibit: influenza virus hemagglutinin protein-mediated viral entry, MARV and EBOV GP1,2-mediated viral entry, SARS-CoV and SARS-CoV-2 S protein-mediated viral entry and VSV G protein-mediated viral entry (PubMed:33563656).

Induces cell cycle arrest and mediates apoptosis by caspase activation and in p53-independent manner. In hepatocytes, IFITM proteins act in a coordinated manner to restrict HCV infection by targeting the endocytosed HCV virion for lysosomal degradation (PubMed:26354436).

IFITM2 and IFITM3 display anti-HCV activity that may complement the anti-HCV activity of IFITM1 by inhibiting the late stages of HCV entry, possibly in a coordinated manner by trapping the virion in the endosomal pathway and targeting it for degradation at the lysosome (PubMed:26354436).
Biological Process
Cellular response to interferon-beta Source: UniProtKB
Defense response to virus Source: GO_Central
Immune response Source: ProtInc
Negative regulation of viral entry into host cell Source: UniProtKB
Negative regulation of viral genome replication Source: UniProtKB
Response to interferon-alpha Source: UniProtKB
Response to interferon-beta Source: UniProtKB
Response to interferon-gamma Source: UniProtKB
Response to virus Source: UniProtKB
Type I interferon signaling pathway Source: GO_Central
Cellular Location
Late endosome membrane; Lysosome membrane; Cell membrane
Topology
Cytoplasmic: 1-56
Helical: 57-77
Cytoplasmic: 78-106
Helical: 107-127
Extracellular: 128-132
PTM
Palmitoylation on membrane-proximal cysteines controls clustering in membrane compartments and antiviral activity.
Phosphorylation at Tyr-19 is required for endosomal and lysosomal location.
More Infomation

Basile, A., Zannella, C., De Marco, M., Sanna, G., Franci, G., Galdiero, M., ... & Marzullo, L. (2023). Spike-mediated viral membrane fusion is inhibited by a specific anti-IFITM2 monoclonal antibody. Antiviral Research, 211, 105546.

Chen, L., Li, X., Deng, Y., Bi, Y., Yan, Z., Yang, Y., ... & Feng, R. (2023). IFITM2 Presents Antiviral Response through Enhancing Type I IFN Signaling Pathway. Viruses, 15(4), 866.

Nchioua, R., Schundner, A., Kmiec, D., Prelli Bozzo, C., Zech, F., Koepke, L., ... & Kirchhoff, F. (2022). SARS-CoV-2 variants of concern hijack IFITM2 for efficient replication in human lung cells. Journal of Virology, 96(11), e00594-22.

Wang, H., Wang, L., Luo, X., Guan, J., Zhang, X., Zhang, L., & Xiang, Y. (2022). Molecular characterization, expression and anti-tumor function analysis of yak IFITM2 gene. International Journal of Biological Macromolecules, 209, 405-412.

Basile, A., Zannella, C., De Marco, M., Franci, G., Galdiero, M., Sanna, G., ... & Marzullo, L. (2022). A specific anti-IFITM2 antibody bars the way to SARS-CoV-2 entry into host cells. bioRxiv, 2022-08.

Winstone, H., Lista, M. J., Reid, A. C., Bouton, C., Pickering, S., Galao, R. P., ... & Neil, S. J. (2021). The polybasic cleavage site in SARS-CoV-2 spike modulates viral sensitivity to type I interferon and IFITM2. Journal of virology, 95(9), 10-1128.

Yang, N., Liu, Z., Pang, S., Wu, J., Liang, J., & Sun, L. (2021). Predicative value of IFITM2 in renal clear cell carcinoma: IFITM2 is associated with lymphatic metastasis and poor clinical outcome. Biochemical and biophysical research communications, 534, 157-164.

Xie, J., Bi, Y., Xu, S., Han, Y., Idris, A., Zhang, H., ... & Feng, R. (2020). Host antiviral protein IFITM2 restricts pseudorabies virus replication. Virus research, 287, 198105.

Alam, U., & Kennedy, D. (2019). G3BP1 and G3BP2 regulate translation of interferon-stimulated genes: IFITM1, IFITM2 and IFITM3 in the cancer cell line MCF7. Molecular and Cellular Biochemistry, 459(1-2), 189-204.

Wang, H., Chen, L., Luo, J., & He, H. (2019). NP and NS1 proteins of H5N1 virus significantly upregulated IFITM1, IFITM2, and IFITM3 in A549 cells. African Health Sciences, 19(1), 1402-1410.

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For research use only. Not intended for any clinical use.

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