Rat Anti-IL10 Recombinant Antibody (AG99) (V2LY-0524-LY142)

Rat

Mouse

AG99

IgG1, κ

CyTOF, Neutralization, WB

Purified Recombinant Mouse IL-10

Rat

Mouse

IgG1, κ

Monoclonal Antibody

< 1.0 EU/mg as determined by the LAL method

Liquid

PBS

None

5 mg/ml

>95% by SDS Page

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Interleukin 10

Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3 (PubMed:16982608).

In turn, STAT3 translocates to the nucleus where it drives expression of anti-inflammatory mediators (PubMed:18025162).

Targets antigen-presenting cells (APCs) such as macrophages and monocytes and inhibits their release of pro-inflammatory cytokines including granulocyte-macrophage colony-stimulating factor /GM-CSF, granulocyte colony-stimulating factor/G-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8 and TNF-alpha (PubMed:1940799, PubMed:7512027, PubMed:11564774).

Interferes also with antigen presentation by reducing the expression of MHC-class II and co-stimulatory molecules, thereby inhibiting their ability to induce T cell activation (PubMed:8144879).

In addition, controls the inflammatory response of macrophages by reprogramming essential metabolic pathways including mTOR signaling (By similarity).

Aging Source: Ensembl
B cell differentiation Source: UniProtKB
B cell proliferation Source: UniProtKB
Branching involved in labyrinthine layer morphogenesis Source: Ensembl
Cellular response to estradiol stimulus Source: Ensembl
Cellular response to hepatocyte growth factor stimulus Source: Ensembl
Cellular response to lipopolysaccharide Source: ARUK-UCL
Cytoplasmic sequestering of NF-kappaB Source: UniProtKB
Defense response to bacterium Source: Ensembl
Defense response to protozoan Source: Ensembl
Endothelial cell apoptotic process Source: BHF-UCL
Hemopoiesis Source: UniProtKB
Leukocyte chemotaxis Source: ProtInc
Liver regeneration Source: Ensembl
Negative regulation of apoptotic process Source: UniProtKB
Negative regulation of autophagy Source: UniProtKB
Negative regulation of B cell proliferation Source: MGI
Negative regulation of cell population proliferation Source: BHF-UCL
Negative regulation of chemokine (C-C motif) ligand 5 production Source: AgBase
Negative regulation of chronic inflammatory response to antigenic stimulus Source: Ensembl
Negative regulation of cytokine activity Source: CACAO
Negative regulation of cytokine production Source: UniProtKB
Negative regulation of cytokine production involved in immune response Source: BHF-UCL
Negative regulation of heterotypic cell-cell adhesion Source: BHF-UCL
Negative regulation of hydrogen peroxide-induced neuron death Source: ARUK-UCL
Negative regulation of inflammatory response Source: BHF-UCL
Negative regulation of interferon-alpha production Source: UniProtKB
Negative regulation of interferon-gamma production Source: Ensembl
Negative regulation of interleukin-12 production Source: AgBase
Negative regulation of interleukin-18 production Source: AgBase
Negative regulation of interleukin-1 production Source: AgBase
Negative regulation of interleukin-6 production Source: BHF-UCL
Negative regulation of interleukin-8 production Source: AgBase
Negative regulation of membrane protein ectodomain proteolysis Source: BHF-UCL
Negative regulation of MHC class II biosynthetic process Source: UniProtKB
Negative regulation of mitotic cell cycle Source: BHF-UCL
Negative regulation of myeloid dendritic cell activation Source: Ensembl
Negative regulation of neuron apoptotic process Source: Ensembl
Negative regulation of nitric oxide biosynthetic process Source: Ensembl
Negative regulation of sensory perception of pain Source: Ensembl
Negative regulation of T cell proliferation Source: UniProtKB
Negative regulation of tumor necrosis factor production Source: AgBase
Negative regulation of vascular associated smooth muscle cell proliferation Source: BHF-UCL
Positive regulation of B cell apoptotic process Source: MGI
Positive regulation of cell cycle Source: Ensembl
Positive regulation of cytokine production Source: BHF-UCL
Positive regulation of DNA-binding transcription factor activity Source: BHF-UCL
Positive regulation of endothelial cell proliferation Source: Ensembl
Positive regulation of heterotypic cell-cell adhesion Source: BHF-UCL
Positive regulation of immunoglobulin production Source: ARUK-UCL
Positive regulation of macrophage activation Source: Ensembl
Positive regulation of MHC class II biosynthetic process Source: Ensembl
Positive regulation of plasma cell differentiation Source: ARUK-UCL
Positive regulation of pri-miRNA transcription by RNA polymerase II Source: ARUK-UCL
Positive regulation of receptor signaling pathway via JAK-STAT Source: UniProtKB
Positive regulation of signaling receptor activity Source: BHF-UCL
Positive regulation of sprouting angiogenesis Source: Ensembl
Positive regulation of transcription, DNA-templated Source: UniProtKB
Positive regulation of vascular associated smooth muscle cell proliferation Source: BHF-UCL
Regulation of gene expression Source: MGI
Regulation of isotype switching Source: UniProtKB
Regulation of response to wounding Source: BHF-UCL
Regulation of synapse organization Source: Ensembl
Response to activity Source: Ensembl
Response to carbon monoxide Source: Ensembl
Response to glucocorticoid Source: BHF-UCL
Response to inactivity Source: Ensembl
Response to insulin Source: Ensembl
Response to molecule of bacterial origin Source: UniProtKB
Response to xenobiotic stimulus Source: Ensembl
Type 2 immune response Source: UniProtKB

Secreted