Mouse Anti-IL10RA Recombinant Antibody (CBT2075) (V2LY-0625-LY1764)

Name: Mouse Anti-IL10RA Recombinant Antibody (CBT2075)
SKU: V2LY-0625-LY1764
Rating: 5 (1 reviews)

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Tested Data

Mouse Anti-IL10RA Recombinant Antibody (CBT2075) (V2LY-0625-LY1764) in ELISA

Mouse Anti-IL10RA Recombinant Antibody (CBT2075) (V2LY-0625-LY1764) in WB

Mouse Anti-IL10RA Recombinant Antibody (CBT2075) (V2LY-0625-LY1764) in IF

Mouse Anti-IL10RA Recombinant Antibody (CBT2075) (V2LY-0625-LY1764) in FC

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBT2075

Application

ICC, FC

Immunogen

Purified recombinant fragment of human CD210 (AA: extra 22-235)) expressed in E. Coli.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG1

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:2,000
ICC	1:25-1:200
FC	1:200-1:400
ELISA	1:10,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Interleukin 10 Receptor Subunit Alpha

Entrez Gene ID

3587

UniProt ID

Q13651

Function

Cell surface receptor for the cytokine IL10 that participates in IL10-mediated anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Upon binding to IL10, induces a conformational change in IL10RB, allowing IL10RB to bind IL10 as well (PubMed:16982608).

In turn, the heterotetrameric assembly complex, composed of two subunits of IL10RA and IL10RB, activates the kinases JAK1 and TYK2 that are constitutively associated with IL10RA and IL10RB respectively (PubMed:12133952).

These kinases then phosphorylate specific tyrosine residues in the intracellular domain in IL10RA leading to the recruitment and subsequent phosphorylation of STAT3. Once phosphorylated, STAT3 homodimerizes, translocates to the nucleus and activates the expression of anti-inflammatory genes. In addition, IL10RA-mediated activation of STAT3 inhibits starvation-induced autophagy (PubMed:26962683).

Biological Process

Cytokine-mediated signaling pathway Source: GO_Central
Negative regulation of autophagy Source: UniProtKB
Positive regulation of receptor signaling pathway via JAK-STAT Source: UniProtKB
Regulation of synapse organization Source: Ensembl
Response to lipopolysaccharide Source: Ensembl
Ubiquitin-dependent endocytosis Source: UniProtKB

Cellular Location

Cell membrane; Cytoplasm

Involvement in disease

Inflammatory bowel disease 28, autosomal recessive (IBD28):
A chronic, relapsing inflammation of the gastrointestinal tract with a complex etiology. It is subdivided into Crohn disease and ulcerative colitis phenotypes. Crohn disease may affect any part of the gastrointestinal tract from the mouth to the anus, but most frequently it involves the terminal ileum and colon. Bowel inflammation is transmural and discontinuous; it may contain granulomas or be associated with intestinal or perianal fistulas. In contrast, in ulcerative colitis, the inflammation is continuous and limited to rectal and colonic mucosal layers; fistulas and granulomas are not observed. Both diseases include extraintestinal inflammation of the skin, eyes, or joints.

Topology

Extracellular: 22-235
Helical: 236-256
Cytoplasmic: 257-578

PTM

Phosphorylated. Phosphorylation of the cytoplasmic tail induced STAT3 activation.
Ubiquitinated by BTRC; ubiquitination leads to endocytosis and subsequent degradation of IL10RA.

Grk, M., Miskovic, R., Jeremic, I., Basaric, M., Dusanovic Pjevic, M., Jekic, B., ... & Banko, A. (2023). Association of IL10RA, IL10RB, and IL22RA Polymorphisms/Haplotypes with Susceptibility to and Clinical Manifestations of SLE. International Journal of Molecular Sciences, 24(14), 11292.

Mario-Vásquez, J. E., Naranjo-González, C. A., Montiel, J., Zuluaga, L. M., Vásquez, A. M., Tobón-Castaño, A., ... & Segura, C. (2021). Association of variants in IL1B, TLR9, TREM1, IL10RA, and CD3G and Native American ancestry on malaria susceptibility in Colombian populations. Infection, Genetics and Evolution, 87, 104675.

Tang, Z., Zhang, P., Ji, M., Yin, C., Zhao, R., Huang, Z., & Huang, Y. (2021). Characterization of novel and large fragment deletions in exon 1 of the IL10RA gene in Chinese children with very early onset inflammatory bowel diseases. BMC gastroenterology, 21(1), 1-8.

Cheng, S., Li, Z., Zhang, W., Sun, Z., Fan, Z., Luo, J., & Liu, H. (2021). Identification of IL10RA by weighted correlation network analysis and in vitro validation of its association with prognosis of metastatic melanoma. Frontiers in Cell and Developmental Biology, 8, 630790.

Prokoph, N., Probst, N. A., Lee, L. C., Monahan, J. M., Matthews, J. D., Liang, H. C., ... & Turner, S. D. (2020). IL10RA modulates crizotinib sensitivity in NPM1-ALK+ anaplastic large cell lymphoma. Blood, The Journal of the American Society of Hematology, 136(14), 1657-1669.

Xue, A. J., Miao, S. J., Sun, H., Qiu, X. X., Wang, S. N., Wang, L., ... & Huang, Y. (2020). Intestinal dysbiosis in pediatric Crohn's disease patients with IL10RA mutations. World journal of gastroenterology, 26(22), 3098.

Al-Abbasi, F. A., Mohammed, K., Sadath, S., Banaganapalli, B., Nasser, K., & Shaik, N. A. (2018). Computational protein phenotype characterization of IL10RA mutations causative to early onset inflammatory bowel disease (IBD). Frontiers in Genetics, 9, 146.

Peng, K., Qian, X., Huang, Z., Lu, J., Wang, Y., Zhou, Y., ... & Huang, Y. (2018). Umbilical cord blood transplantation corrects very early-onset inflammatory bowel disease in Chinese patients with IL10RA-associated immune deficiency. Inflammatory Bowel Diseases, 24(7), 1416-1427.

Zadka, Ł., Kulus, M. J., Kurnol, K., Piotrowska, A., Glatzel-Plucińska, N., Jurek, T., ... & Dzięgiel, P. (2018). The expression of IL10RA in colorectal cancer and its correlation with the proliferation index and the clinical stage of the disease. Cytokine, 110, 116-125.

Jung, E. S., Petersen, B. S., Mayr, G., Cheon, J. H., Kang, Y., Lee, S. J., ... & Koh, H. (2018). Compound heterozygous mutations in IL10RA combined with a complement factor properdin mutation in infantile-onset inflammatory bowel disease. European Journal of Gastroenterology & Hepatology, 30(12), 1491-1496.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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For research use only. Not intended for any clinical use.

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